Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,365,433	T→C	E63G (GAA→GGA)	ais ←	putative lipopolysaccharide core heptose(II)‑phosphate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,365,433	0	T	C	100.0%	176.8 / NA	49	E63G (GAA→GGA)	ais	putative lipopolysaccharide core heptose(II)‑phosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/22);  total (27/22)

TATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTTCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCTACTCCAGGCGGCATGAGTACCCAGTCCGGCAATTGCAGCGAG  >  NC_000913/2365299‑2365560                                                                                                                                       |                                                                                                                                tATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGTTTGGTTGAACGGTCGCAACGTCCAGCa                                                                                                                            >  2:819623/1‑140 (MQ=255)  aTTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCAt                                                                                                                           >  1:518383/1‑140 (MQ=255)           aTAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGTATGACGAAACa                                                                                                                  >  2:95563/1‑140 (MQ=255)           aTAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACa                                                                                                                  >  1:282859/1‑140 (MQ=255)           aTAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACa                                                                                                                  >  1:776416/1‑140 (MQ=255)           aTAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACa                                                                                                                  >  2:799393/1‑140 (MQ=255)              aaGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACaaaa                                                                                                               <  2:282859/140‑1 (MQ=255)                      aaTCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAAcc                                                                                                       <  2:181327/140‑1 (MQ=255)                            ggATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGtgc                                                                                                 >  1:631111/1‑140 (MQ=255)                              atatCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGtgctg                                                                                               <  1:375265/140‑1 (MQ=255)                                  cAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCtgt                                                                                           >  2:954779/1‑140 (MQ=255)                                     cACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGcc                                                                                        >  1:34819/1‑140 (MQ=255)                                      aCTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCa                                                                                       >  2:347971/1‑140 (MQ=255)                                       cTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGtgctg                                                                                               <  2:97016/131‑1 (MQ=255)                                       cTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGtgctg                                                                                               >  1:97016/1‑131 (MQ=255)                                        tAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGt                                                                                     <  1:799393/140‑1 (MQ=255)                                             gCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGc                                                                                <  2:34819/140‑1 (MQ=255)                                             gCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGc                                                                                <  1:954779/140‑1 (MQ=255)                                               gTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGtgctg                                                                                               <  1:107767/123‑1 (MQ=255)                                               gTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGtgctg                                                                                               >  2:107767/1‑123 (MQ=255)                                               gTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCa                                                                              >  2:886553/1‑140 (MQ=255)                                               gTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCa                                                                              <  1:347971/140‑1 (MQ=255)                                                      aGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGtttt                                                                       >  2:33285/1‑140 (MQ=255)                                                                       ggTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCa                                                      <  1:926781/140‑1 (MQ=255)                                                                          aCCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAAc                                                   <  1:36994/140‑1 (MQ=255)                                                                             tttAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCAt                                                >  2:366179/1‑140 (MQ=255)                                                                                 aCCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCt                                            >  1:80793/1‑140 (MQ=255)                                                                                 aCCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCt                                            >  1:906167/1‑140 (MQ=255)                                                                                      aaTACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCTACTcc                                       >  1:560292/1‑140 (MQ=255)                                                                                          ccTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCTACTCCAggc                                   <  1:886553/140‑1 (MQ=255)                                                                                            tGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGTATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCTACTCCAggcgg                                 <  1:95563/140‑1 (MQ=255)                                                                                            tGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCTACTCCAggcgg                                 <  1:490538/140‑1 (MQ=255)                                                                                             gTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCTACTCCAggcggc                                >  2:42717/1‑140 (MQ=255)                                                                                               tttATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCTACTCCAGGCGGCAt                              >  2:597010/1‑140 (MQ=255)                                                                                               tttATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCAATGCTACTCCAGGCGGCAt                              >  2:913010/1‑140 (MQ=255)                                                                                                ttATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCTACTCCAGGCGGCATg                             <  2:80793/140‑1 (MQ=255)                                                                                                       aCAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCTACTCCAGGCGGCATGAGTAccc                      >  2:237530/1‑140 (MQ=255)                                                                                                        cAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCTACTCCAggcgg                                 <  2:617776/128‑1 (MQ=255)                                                                                                        cAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCTACTCCAggcgg                                 >  1:617776/1‑128 (MQ=255)                                                                                                        cAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCTACTCCAGGCGGCATGAGTAcc                       <  2:208571/138‑1 (MQ=255)                                                                                                        cAAGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCTACTCCAGGCGGCATGAGTAcc                       >  1:208571/1‑138 (MQ=255)                                                                                                          aGCATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCTACTCCAGGCGGCATGAGTACCCAGt                   <  1:667513/140‑1 (MQ=255)                                                                                                            cATTGATTGGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCTACTCCAGGCGGCATGAGTACCCAGTcc                 <  1:42717/140‑1 (MQ=255)                                                                                                                gattgGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCTACTCCAGGCGGCATGAGTACCCAGTCCGGCa             <  2:560292/140‑1 (MQ=255)                                                                                                                   tgGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCTACTCCAGGCGGCATGAGTa                         >  2:189185/1‑125 (MQ=255)                                                                                                                   tgGTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCTACTCCAGGCGGCATGAGTa                         <  1:189185/125‑1 (MQ=255)                                                                                                                     gTTGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCTACTCCAGGCGGCATGAGTACCCAGTCCGGCAATTGc        >  2:714422/1‑140 (MQ=255)                                                                                                                      ttGAACGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCTACTCCAGGCGGCATGAGTACCCAGTCCGGCAATTGCa       >  1:131783/1‑140 (MQ=255)                                                                                                                           cGGTCGCAACGTCCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCTACTCCAGGCGGCATGAGTACCCAGTCCGGCAATTGCagcgag  <  1:714422/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                TATTACTGGAATAAAGATCGAAATCAGGGATATCAGCACTAAAAGCGTTGCCCAGTTCACGGGCATCCTGGGTACCTTTAACCGTAATACCTGTTTTATCTGACAAGCATTGATTGGTTGAACGGTCGCAACGTTCAGCATGACGAAACAAAACGACAACCGGGTGCTGCTGTGCCAGTCTGGCCAGTGTTTTATTGTCGATACGTGGCAAACCATTGCTACTCCAGGCGGCATGAGTACCCAGTCCGGCAATTGCAGCGAG  >  NC_000913/2365299‑2365560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑