Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,462,832	T→C	intergenic (+186/‑180)	fadL → / → yfdF	long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,462,832	0	T	C	100.0%	142.0 / NA	40	intergenic (+186/‑180)	fadL/yfdF	long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/20);  total (20/20)

CCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAT  >  NC_000913/2462698‑2462965                                                                                                                                       |                                                                                                                                      cccACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCt                                                                                                                                  >  1:69546/1‑140 (MQ=255)    aCTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGt                                                                                                                               <  1:494436/140‑1 (MQ=255)       tCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCt                                                                                                                            >  2:16581/1‑140 (MQ=255)       tCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCt                                                                                                                            >  1:850878/1‑140 (MQ=255)            gAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCa                                                                                                                       <  1:809137/140‑1 (MQ=255)                  agtGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGt                                                                                                                 >  2:14701/1‑140 (MQ=255)                    tgtCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGttt                                                                                                               >  1:77124/1‑140 (MQ=255)                        cTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACtt                                                                                                           >  1:813829/1‑140 (MQ=255)                        cTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACtt                                                                                                           >  2:772192/1‑140 (MQ=255)                          cATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACtttt                                                                                                         >  2:355033/1‑140 (MQ=255)                             tGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTg                                                                                                      <  1:355033/140‑1 (MQ=255)                              gCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGc                                                                                                     <  1:698056/140‑1 (MQ=255)                                  aCCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCAttt                                                                                                 >  1:595907/1‑140 (MQ=255)                                  aCCTCCTGAGTTTT‑CAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTc                                                                                                >  2:754157/1‑140 (MQ=255)                                        tGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGa                                                                                           <  1:928080/140‑1 (MQ=255)                                         gAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAt                                                                                          >  2:703924/1‑140 (MQ=255)                                               tgtaaaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTccc                                                                                    <  2:595907/137‑1 (MQ=255)                                               tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTccc                                                                                    <  1:14701/140‑1 (MQ=255)                                                   aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTccccccc                                                                                <  2:850878/140‑1 (MQ=255)                                                   aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTccccccc                                                                                <  1:16581/140‑1 (MQ=255)                                                    aCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccc                                                                               <  1:703924/140‑1 (MQ=255)                                                    aCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTcccccccc                                                                               <  1:491159/140‑1 (MQ=255)                                                              ggCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATtg                                                                     <  1:481416/140‑1 (MQ=255)                                                                cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTTCTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATtgtg                                                                   <  2:625014/140‑1 (MQ=255)                                                                  gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGCCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTgcg                                                                 <  2:676481/140‑1 (MQ=255)                                                                             cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTc                                                      <  2:252864/140‑1 (MQ=255)                                                                                    aTAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTaa                                               >  1:219017/1‑140 (MQ=255)                                                                                            tATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGt                                       >  2:938634/1‑140 (MQ=255)                                                                                             aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCaaa                                                   <  1:82324/127‑1 (MQ=255)                                                                                             aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCaaa                                                   >  2:82324/1‑127 (MQ=255)                                                                                             aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                      <  2:384490/140‑1 (MQ=255)                                                                                                    gACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaata                               >  1:63606/1‑140 (MQ=255)                                                                                                         aCCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                           >  2:282151/1‑123 (MQ=255)                                                                                                         aCCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                           <  1:282151/123‑1 (MQ=255)                                                                                                         aCCTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGa                          >  1:148018/1‑140 (MQ=255)                                                                                                          ccTGAAGGGAAAGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                         >  1:414215/1‑140 (MQ=255)                                                                                                                   aaaGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTg                >  2:522943/1‑140 (MQ=255)                                                                                                                   aaaGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTg                >  2:377697/1‑140 (MQ=255)                                                                                                                    aaGGCATATTTTCAGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa               <  2:414215/140‑1 (MQ=255)                                                                                                                                 aGGCGTCCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt  <  2:148018/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      CCCACTTCACCGAAAGTAGTGTCCTCATTGCTTACCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGATACTCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAT  >  NC_000913/2462698‑2462965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑