Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,502,049 A→G F813L (TTT→CTT)  fryA ← putative PTS multiphosphoryl transfer protein FryA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,502,0490AG100.0% 71.2 / NA 21F813L (TTT→CTT) fryAputative PTS multiphosphoryl transfer protein FryA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/10);  total (11/10)

CGACTATTTTTGTCGCGTTTTCCGGCGTCAGTTTGGTCTGACGCCGGGAGAGTACAGCGCCCGTTTTCAGGGCTAACGTCAGAAGGTTAATTCTGTTTCCAGCAGCGTCAGGATACTTTGTGCATCTTGCGCGGCAAACAACGACTGGCGGAAGTTTTTATTCACCAGTTTACGCGCCAGCTGCGAGAAGACTTTCACATGATTCATCCCTTCGTTAGCACCCAGCGTCAGCATGATCACCAGTTCGACTTCGCCCATTTCTGACTGC  >  NC_000913/2501912‑2502179
                                                                                                                                         |                                                                                                                                  
cGACTATTTTTGTCGCGTTTTCCGGCGTCAGTTTGGTCTGACGCCGGGAGAGTACAGCGCCCGTTTTCAGGGCTAACGTCAGAAGGTTAATTCAGTTTCCAGCAGCGTCAGGATACTTTGTGCATCTTGCGCGGCAagca                                                                                                                                  >  1:695657/1‑140 (MQ=255)
                              gTTTGGTCTGACGCCGGGAGAGTACAGCGCCCGTTTTCAGGGCTAACGTCAGAAGGTTAATTCTGTTTCCAGCAGCGTCAGGATACTTTGTGCATCTTGCGCGGCAAGCAACGACTGGCGGAAGTTTTTATTCACCAGtt                                                                                                    <  2:610337/140‑1 (MQ=255)
                                       gACGCCGGGAGAGTACAGCGCCCGTTTTCAGGGCTAACGTCAGAAGGTTAATTCTGTTTCCAGCAGCGTCAGGATACTTTGTGCATCTTGCGCGGCAAGCAACGACTGGCGGAAGTTTTTATTCACCAGTTTACGCGCCa                                                                                           >  1:869256/1‑140 (MQ=255)
                                                         cgcCCGTTTTCAGGGCTAACGTCAGAAGGTTAATTCTGTTTCCAGCAGCGTCAGGATACTTTGTGCATCTTGCGCGGCAAGCAACGACTGGCGGAAGTTTTTATTCACCAGTTTACGCGCCAGCTGCGAGAAGACTTTca                                                                         >  2:962206/1‑140 (MQ=255)
                                                               ttttCAGGGCTAACGTCAGAAGGTTAATTCTGTTTCCAGCAGCGTCAGGACACTTTGTGCATCTTGCGCGGCAAGCAACGACTGGCGGAAGTTTTTATTCACCAGTTTACGCGCCAGCTGCGAGAAGACTTTCACATGAt                                                                   <  2:869256/140‑1 (MQ=255)
                                                                    aGGGCTAACGTCAGAAGGTTAATTCTGTTTCCAGCAGCGTCAGGATACTTTGTGCATCTTGCGCGGCAAGCAACGACTGGCGGAAGTTTTTATTCACCAGTTTACGCGCCAGCTGCGAGAAGACTTTCACATGATTCATc                                                              <  1:129908/140‑1 (MQ=255)
                                                                           aCGTCAGAAGGTTAATTCTGTTTCCAGCAGCGTCAGGATACTTTGTGCATCTTGCGCGGCAAGCAACGACTGGCGGAAGTTTTTATTCACCAGTTTACGCGCCAGCTGCGAGAAGACTTTCACATGATTCATCCCTTCGt                                                       >  2:93748/1‑140 (MQ=255)
                                                                                       tAATTCTGTTTCCAGCAGCGTCAGGATACTTTGTGCATCTTGCGCGGCAAGCAACGACTGGCGGAAGTTTTTATTCACCAGTTTACGCGCCAGCTGCGAGAAGACTTTCACATGATTCATCCCTTCGTTAGCACCCAGCg                                           >  2:426622/1‑140 (MQ=255)
                                                                                          ttCTGTTTCCAGCAGCGTCAGGATACTTTGTGCATCTTGCGCGGCAAGCAACGACTGGCGGAAGTTTTTATTCACCAGTTTACGCGCCAGCTGCGAGAAGACTTTCACATGATTCATCCCTTCGTTAGCACCCAGCGTCa                                        <  1:426622/140‑1 (MQ=255)
                                                                                               tttCCAGCAGCGTCAGGATACTTTGTGCATCTTGCGCGGCAAGCAACGACTGGCGGAAGTTTTTATTCACCAGTTTACGCGCCAGCTGCGAGAAGACTTTCACATGATTCATCCCTTCGTTAGCACCCAGCGTCAGCAt                                    <  2:25178/139‑1 (MQ=255)
                                                                                               tttCCAGCAGCGTCAGGATACTTTGTGCATCTTGCGCGGCAAGCAACGACTGGCGGAAGTTTTTATTCACCAGTTTACGCGCCAGCTGCGAGAAGACTTTCACATGATTCATCCCTTCGTTAGCACCCAGCGTCAGCAt                                    >  1:25178/1‑139 (MQ=255)
                                                                                                         cGTCAGGATACTTTGTGCATCTTGCGCGGCAAGCAACGACTGGCGGAAGTTTTTATTCACCAGTTTACGCGCCAGCTGCGAGAAGACTTTCACATGATTCATCCCTTCGTTAGCACCCAGCGTCAGCATGATCACCAGtt                         >  1:387869/1‑140 (MQ=255)
                                                                                                           tCAGGATACTTTGTGCATCTTGCGCGGCAAGCAACGACTGGCGGAAGTTTTTATTCACCAGTTTACGCGCCAGCTGCGAGAAGACTTTCACATGATTCATCCCTTCGTTAGCACCCAGCGTCAGCATGATCACCAGTTCg                       >  1:609499/1‑140 (MQ=255)
                                                                                                                aTACTTTGTGCATCTTGCGCGGGAAGCAACGACTGGCGGAAGTTTTTATTCACCAGTTTACGCGCCAGCTGCGAGAAGACTTTCACATGATTCATCCCTTCGTTAGCACCCAGCGTCAGCATGATCACCAGTTCGACTTc                  <  2:794827/140‑1 (MQ=255)
                                                                                                                      tgtgCATCTTGCGCGGCAAGCAACGACTGGCGGAAGTTTTTATTCACCAGTTTACGCGCCAGCTGCGAGAAGACTTTCACATGATTCATCCCTTCGTTAGCACCCAGCGTCAGCATGATCAc                              <  2:144120/122‑1 (MQ=255)
                                                                                                                      tgtgCATCTTGCGCGGCAAGCAACGACTGGCGGAAGTTTTTATTCACCAGTTTACGCGCCAGCTGCGAGAAGACTTTCACATGATTCATCCCTTCGTTAGCACCCAGCGTCAGCATGATCAc                              >  1:144120/1‑122 (MQ=255)
                                                                                                                        tgCATCTTGCGCGGCAAGCAACGACTGGCGGAAGTTTTTATTCACGAGTTTACGCGCCAGCTGCGAGAAGACTTTCACATGATTCATCCCTTCGTTAGCACCCAGCGTCAGCATGATCACCAGTTCGACTTCGCCCAttt          >  2:906548/1‑140 (MQ=255)
                                                                                                                          cATCTTGCGCGGCAAGCAACGACTGGCGGAAGTTTTTATTCACCAGTTTACGCGCCAGCTGCGAGAAGACTTTCACATGATTCATCCCTTCGTTAGCACCCAGCGTCAGCATGATCACCAGTTCGACTTCGCCCATTTCt        <  2:62736/140‑1 (MQ=255)
                                                                                                                              ttGCGCGGCAAGCAACGACTGGCGGAAGTTTTTATTCACCAGTTTACGCGCCAGCTGCGAGAAGACTTTCACATGATTCATCCCTTCGTTAGCACCCAGCGTCAGCATGATCACCAGTTCGACTTCGCCCATTTCTGACt    >  2:735583/1‑140 (MQ=255)
                                                                                                                                gcgcGGCAAGCAACGACTGGCGGAAGTTTTTATTCACCAGTTTACGCGCCAGCTGCGAGAAGACTTTCACATGATTCATCCCTTCGTTAGCACCCAGCGTCAGCATGATCACCAGTTCGACTTCGCCCATTTCTGACTgc  <  1:735583/140‑1 (MQ=255)
                                                                                                                                  gcgGCAAGCAACGACTGGCGGAAGTTTTTATTCACCAGTTTACGCGCCAGCTGCGAGAAGACTTTCACATGATTCATCCCTTCGTTAGCACCCAGCGTCAGCATGATCACCAGTTCGACTTCGccc              <  1:690980/126‑1 (MQ=255)
                                                                                                                                  gcgGCAAGCAACGACTGGCGGAAGTTTTTATTCACCAGTTTACGCGCCAGCTGCGAGAAGACTTTCACATGATTCATCCCTTCGTTAGCACCCAGCGTCAGCATGATCACCAGTTCGACTTCGccc              >  2:690980/1‑126 (MQ=255)
                                                                                                                                         |                                                                                                                                  
CGACTATTTTTGTCGCGTTTTCCGGCGTCAGTTTGGTCTGACGCCGGGAGAGTACAGCGCCCGTTTTCAGGGCTAACGTCAGAAGGTTAATTCTGTTTCCAGCAGCGTCAGGATACTTTGTGCATCTTGCGCGGCAAACAACGACTGGCGGAAGTTTTTATTCACCAGTTTACGCGCCAGCTGCGAGAAGACTTTCACATGATTCATCCCTTCGTTAGCACCCAGCGTCAGCATGATCACCAGTTCGACTTCGCCCATTTCTGACTGC  >  NC_000913/2501912‑2502179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑