Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,574,588	T→C	E139G (GAG→GGG)	eutQ ←	putative ethanolamine utilization protein EutQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,574,588	0	T	C	100.0%	160.1 / NA	45	E139G (GAG→GGG)	eutQ	putative ethanolamine utilization protein EutQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/22);  total (23/22)

ACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCTCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAGCTCGGCTGCATTGCGCCCTGCTCCAGCGACTGTTTTTCCTTCATCACTTTTTCCAT  >  NC_000913/2574462‑2574724                                                                                                                               |                                                                                                                                         aCCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGaa                                                                                                                             <  2:6890/140‑1 (MQ=255)     tGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGa                                                                                                                         >  2:926893/1‑140 (MQ=255)     tGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGa                                                                                                                         >  1:63360/1‑140 (MQ=255)     tGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGa                                                                                                                         >  1:395100/1‑140 (MQ=255)     tGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGa                                                                                                                         >  2:739449/1‑140 (MQ=255)     tGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGa                                                                                                                         >  1:544430/1‑140 (MQ=255)                 cgtAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACg                                                                                                             >  2:555628/1‑140 (MQ=255)                            gTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCatc                                                                                                  >  1:824300/1‑140 (MQ=255)                               cACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCAAAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCatcaat                                                                                               >  2:904893/1‑140 (MQ=255)                                aCGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCatcaatc                                                                                              >  2:49741/1‑140 (MQ=255)                                  gggAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCAc                                                                                            >  2:154102/1‑140 (MQ=255)                                         aTGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATg                                                                                     >  2:150134/1‑140 (MQ=255)                                           gCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGcc                                                                                                     <  1:375649/122‑1 (MQ=255)                                           gCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGcc                                                                                                     >  2:375649/1‑122 (MQ=255)                                             gTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATgccgc                                                                                 <  1:49741/140‑1 (MQ=255)                                               tctcCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCt                                                                               <  2:544430/140‑1 (MQ=255)                                               tctcCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCt                                                                               <  2:395100/140‑1 (MQ=255)                                                ctcCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCtt                                                                              >  1:409024/1‑140 (MQ=255)                                                  cccACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTg                                                                            >  1:464047/1‑140 (MQ=255)                                                      cTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCgg                                                                        >  1:786856/1‑140 (MQ=255)                                                         cATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATgccgc                                                                                 <  1:909013/128‑1 (MQ=255)                                                         cATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATgccgc                                                                                 >  2:909013/1‑128 (MQ=255)                                                          aTAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCAc                                                                    <  1:926893/140‑1 (MQ=255)                                                           tAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCAcc                                                                   <  1:739449/140‑1 (MQ=255)                                                                   gcggcCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGaa                                                           >  1:685342/1‑140 (MQ=255)                                                                    cggcCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAg                                                          <  1:23869/140‑1 (MQ=255)                                                                         aTGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAGCTCgg                                                     <  2:685342/140‑1 (MQ=255)                                                                         aTGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAGCTCgg                                                     <  1:154102/140‑1 (MQ=255)                                                                              gctgcCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAGCTCGGCTGCa                                                <  2:786856/140‑1 (MQ=255)                                                                               ctgcCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAGCTCGGCTGCAt                                               >  2:938392/1‑140 (MQ=255)                                                                                     tcgtcTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAGCTCGGCTGCATTGCGcc                                         >  2:397861/1‑140 (MQ=255)                                                                                     tcgtcTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAGCTCGGCTGCATTGCGcc                                         <  2:415332/140‑1 (MQ=255)                                                                                                cacaAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAGCTCGGCTGCATTGCGCCCTGCTCCAGCg                              <  2:398096/140‑1 (MQ=255)                                                                                                 acaAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAGCTCGGCTGCATTGCGCCCTGCTCCAGCGa                             <  1:397861/140‑1 (MQ=255)                                                                                                 acaAGATCAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAGCTCGGCTGCATTGCGCCCTGCTCCAGCGa                             <  1:938392/140‑1 (MQ=255)                                                                                                        cAGTCAAGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAGCTCGGCTGCATTGCGCCCTGCTCCAGCGACTGtttt                      <  1:150134/140‑1 (MQ=255)                                                                                                         aGTCAAGCCCACGCAATGTGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAGCTCGGCTGCATTGCGCCCTGCTCCAGCGACTGttttt                     >  1:582190/1‑140 (MQ=255)                                                                                                             aaGCCCACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAGCTCGGCTGCATTGCGCCCTGCTCCAGCGACTGTTTTTCCtt                 >  1:811892/1‑140 (MQ=255)                                                                                                                cccACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAGCTCGGCTGCATTGCGCCCTGCTCCAGCGACTGTTTTTCCTtcat              <  2:63360/140‑1 (MQ=255)                                                                                                                 ccACGCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAGCTCGGCTGCATTGCGCCCTGCTCCAGCGACTGTTTTTCCTtcatc             <  1:439965/140‑1 (MQ=255)                                                                                                                     gCAATGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAGCTCGGCTGCATTGCGCCCTGCTCCAGCGACTGTTTTTCCTTCATCACtt         <  1:555628/140‑1 (MQ=255)                                                                                                                         tGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAGCTCGGCTGCATTGCGCCCTGCTCCAGCGACTGTTTTTcc                   >  1:825209/1‑126 (MQ=255)                                                                                                                         tGCGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAGCTCGGCTGCATTGCGCCCTGCTCCAGCGACTGTTTTTcc                   <  2:825209/126‑1 (MQ=255)                                                                                                                           cGGCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAGCTCGGCTGCATTGCGCCCTGCTCCAGCGACTGTTTTTCCTTCATCACTTTTTCCa   >  1:839243/1‑140 (MQ=255)                                                                                                                            ggCCCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAGCTCGGCTGCATTGCGCCCTGCTCCAGCGACTGTTTTTCCTTCATCACTTTTTCCAt  <  2:824300/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                         ACCATGTCGATTTCGTCGTAGTTCAGGGTCCACGGGAAGAATGCGTTCTCCCACTGCATAAAACCGGCGGCCATGCTGCTGCCGTCGTCTCCGGTCACAAGATCAGTCAAGCCCACGCAATGCGGCTCTGCGCCGTCGAAACGACCAAACTTGACGCTACTGCCATCAATCACTTTGATGCCGCCTTTGCCGGTCACCGATTTGAAGCTCGGCTGCATTGCGCCCTGCTCCAGCGACTGTTTTTCCTTCATCACTTTTTCCAT  >  NC_000913/2574462‑2574724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑