Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,698,405	G→A	A49V (GCC→GTC)	pgpC ←	phosphatidylglycerophosphatase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,698,405	0	G	A	100.0%	161.9 / NA	46	A49V (GCC→GTC)	pgpC	phosphatidylglycerophosphatase C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/24);  total (22/24)

ACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGGCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCT  >  NC_000913/2698270‑2698537                                                                                                                                        |                                                                                                                                     aCATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACtat                                                                                                                                  >  1:818514/1‑140 (MQ=255)  cATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACtata                                                                                                                                 <  2:818514/140‑1 (MQ=255)  cATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACtata                                                                                                                                 <  2:152211/140‑1 (MQ=255)     tGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATc                                                                                                                              <  1:525008/140‑1 (MQ=255)                   cgcACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAg                                                                                                                >  2:281272/1‑140 (MQ=255)                             cGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAAc                                                                                                      >  1:361956/1‑140 (MQ=255)                                     aaCGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAg                                                                                              <  2:330064/140‑1 (MQ=255)                                     aaCGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCACACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAg                                                                                              <  1:335356/140‑1 (MQ=255)                                            gTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTgg                                                                                       <  2:361956/140‑1 (MQ=255)                                            gTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTgg                                                                                       <  1:909664/140‑1 (MQ=255)                                              aaaCGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCg                                                                                     <  1:281272/140‑1 (MQ=255)                                              aaaCGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCg                                                                                     <  2:797887/140‑1 (MQ=255)                                              aaaCGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCg                                                                                     <  1:832324/140‑1 (MQ=255)                                              aaaCGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCg                                                                                     <  1:154815/140‑1 (MQ=255)                                              aaaCGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAACAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCg                                                                                     <  2:672482/140‑1 (MQ=255)                                                 cGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACg                                                                                  >  2:636616/1‑140 (MQ=255)                                                   tGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCg                                                                                     >  2:571952/1‑135 (MQ=255)                                                   tGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCg                                                                                     <  1:788611/135‑1 (MQ=255)                                                   tGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCg                                                                                     <  1:571952/135‑1 (MQ=255)                                                   tGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCg                                                                                     >  2:788611/1‑135 (MQ=255)                                                   tGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGAc                                                                                   >  2:405342/1‑137 (MQ=255)                                                   tGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGAc                                                                                   <  1:405342/137‑1 (MQ=255)                                                    gCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACgtag                                                                               >  2:707710/1‑140 (MQ=255)                                                     cTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACgtagt                                                                              >  2:77975/1‑140 (MQ=255)                                                     cTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACgtagt                                                                              >  2:713205/1‑140 (MQ=255)                                                       tCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCg                                                                                     >  1:416753/1‑131 (MQ=255)                                                       tCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCg                                                                                     <  2:416753/131‑1 (MQ=255)                                                             tgACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAgcg                                                                      <  1:636616/140‑1 (MQ=255)                                                               aCCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCa                                                                    >  1:303007/1‑140 (MQ=255)                                                                       tGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTg                                                            <  1:747044/140‑1 (MQ=255)                                                                       tGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTg                                                            <  1:713205/140‑1 (MQ=255)                                                                         cACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGcc                                                          >  1:509737/1‑140 (MQ=255)                                                                                   gAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATcc                                                <  1:34869/140‑1 (MQ=255)                                                                                          aCTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGAt                                         >  1:584348/1‑140 (MQ=255)                                                                                              aTCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCaa                                     >  2:415715/1‑140 (MQ=255)                                                                                              aTCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCaa                                     >  2:504031/1‑140 (MQ=255)                                                                                                    cAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCctgctg                                           >  1:957589/1‑128 (MQ=255)                                                                                                    cAGCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCctgctg                                           <  2:957589/128‑1 (MQ=255)                                                                                                      gCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCa                                      <  2:776161/131‑1 (MQ=255)                                                                                                      gCGTGCCGCACGACCTTTTATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCa                                      >  1:776161/1‑131 (MQ=255)                                                                                                                      tttATCAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAacac             <  2:370188/140‑1 (MQ=255)                                                                                                                           cAATAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAAAGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGTTGCCATGTTCCCTCTAAGTCAAAAAACACCacac        >  2:561075/1‑140 (MQ=255)                                                                                                                             aTAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACGCCACacga      <  1:561075/140‑1 (MQ=255)                                                                                                                             aTAACGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACacga      >  2:743299/1‑140 (MQ=255)                                                                                                                                 cGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCt  >  2:522827/1‑140 (MQ=255)                                                                                                                                 cGCAATGACTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCt  >  2:119139/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     ACATTGTCGCGAAACCAGCGCACGAAATCGGCCTGCAACGTCTGTAAACGTGCTTCGCTGTGACCAAAAGTGCACCCCCACAGAAGCAGACTCATCGGCCAGCGTGCCGCACGACCTTTTATCAATAACGCAATGGCTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCT  >  NC_000913/2698270‑2698537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑