Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	395,690	C→T	G200S (GGC→AGC)	ampH ←	peptidoglycan DD‑carboxypeptidase/peptidoglycan DD‑endopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	395,690	0	C	T	100.0%	293.7 / NA	81	G200S (GGC→AGC)	ampH	peptidoglycan DD‑carboxypeptidase/peptidoglycan DD‑endopeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (48/33);  total (48/33)

ATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACC  >  NC_000913/395559‑395826                                                                                                                                    |                                                                                                                                         aTTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCAt                                                                                                                                  <  1:28620/140‑1 (MQ=255)  ttGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCAtt                                                                                                                                 <  1:475322/140‑1 (MQ=255)     cACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGc                                                                                                                              >  1:46158/1‑140 (MQ=255)      aCGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGcc                                                                                                                             >  1:839906/1‑140 (MQ=255)       cGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCa                                                                                                                            <  2:99305/140‑1 (MQ=255)       cGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCa                                                                                                                            >  1:910067/1‑140 (MQ=255)         gACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCa                                                                                                                                   <  2:903478/131‑1 (MQ=255)         gACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCa                                                                                                                                   >  1:903478/1‑131 (MQ=255)          aCTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTg                                                                                                                         <  2:46158/140‑1 (MQ=255)          aCTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTg                                                                                                                         <  1:605488/140‑1 (MQ=255)              gCACCGCGCTCAGCAACCATTAAACGGCGGCACTGCTCCGGGGAGGGGGCGTAGGCGGCGGCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATc                                                                                                                     >  2:523887/1‑140 (MQ=255)                    cgcTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTTGCCAGTGCATCTGCcag                                                                                                               >  2:860697/1‑140 (MQ=255)                    cgcTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGc                                                                                                                              >  1:635333/1‑125 (MQ=255)                    cgcTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGc                                                                                                                              <  2:635333/125‑1 (MQ=255)                    cgcTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCcag                                                                                                               >  2:494263/1‑140 (MQ=255)                    cgcTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCcag                                                                                                               >  2:199990/1‑140 (MQ=255)                    cgcTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCcag                                                                                                               >  2:811272/1‑140 (MQ=255)                      cTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCcagca                                                                                                             >  1:883351/1‑140 (MQ=255)                               cATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACg                                                                                                    <  2:118178/140‑1 (MQ=255)                                aTTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGc                                                                                                   <  2:274307/140‑1 (MQ=255)                                aTTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGc                                                                                                   <  2:883351/140‑1 (MQ=255)                                 ttAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCa                                                                                                  >  2:762538/1‑140 (MQ=255)                                        gcggcACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTag                                                                                           >  1:694293/1‑140 (MQ=255)                                         cggcACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGc                                                                                                   >  2:354092/1‑131 (MQ=255)                                         cggcACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGc                                                                                                   <  1:354092/131‑1 (MQ=255)                                         cggcACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTaga                                                                                          >  2:163258/1‑140 (MQ=255)                                         cggcACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTaga                                                                                          >  2:160860/1‑140 (MQ=255)                                         cggcACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTaga                                                                                          >  1:446071/1‑140 (MQ=255)                                              cTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTAcg                                                                                     <  1:494263/140‑1 (MQ=255)                                                           ggggTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGcc                                                                        >  2:846075/1‑140 (MQ=255)                                                                gtAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGgcg                                                                   <  1:199990/140‑1 (MQ=255)                                                                gtAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGgcg                                                                   <  1:211344/140‑1 (MQ=255)                                                                 tAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTAcgccg                                                                                  >  1:846831/1‑124 (MQ=255)                                                                 tAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTAcgccg                                                                                  <  2:846831/124‑1 (MQ=255)                                                                 tAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGgcgc                                                                  <  2:446071/140‑1 (MQ=255)                                                                      ggtgTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCtt                                                             >  2:270785/1‑140 (MQ=255)                                                                       gtgtCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCttt                                                            >  2:539902/1‑140 (MQ=255)                                                                       gtgtCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCttt                                                            >  2:272396/1‑140 (MQ=255)                                                                        tgtCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTc                                                           >  1:544341/1‑140 (MQ=255)                                                                        tgtCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTc                                                           >  2:199461/1‑140 (MQ=255)                                                                        tgtCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTc                                                           >  1:59624/1‑140 (MQ=255)                                                                         gtCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCa                                                          >  1:523409/1‑140 (MQ=255)                                                                         gtCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCa                                                          >  1:407760/1‑140 (MQ=255)                                                                           cTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGc                                                        >  2:295236/1‑140 (MQ=255)                                                                            tttCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCt                                                       >  1:345522/1‑140 (MQ=255)                                                                            tttCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCt                                                       <  1:846075/140‑1 (MQ=255)                                                                             ttCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCtt                                                      <  2:694293/140‑1 (MQ=255)                                                                              tCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTc                                                     >  1:163377/1‑140 (MQ=255)                                                                               cATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCg                                                    <  1:539902/140‑1 (MQ=255)                                                                                    ccAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTa                                               >  2:525601/1‑140 (MQ=255)                                                                                      aaTGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCg                                                 <  2:214889/136‑1 (MQ=255)                                                                                      aaTGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCg                                                 >  1:214889/1‑136 (MQ=255)                                                                                      aaTGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGa                                             <  1:811272/140‑1 (MQ=255)                                                                                      aaTGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGa                                             <  2:839906/140‑1 (MQ=255)                                                                                        tGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGaaa                                           <  2:835481/140‑1 (MQ=255)                                                                                           aCGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGt                                        <  2:59624/140‑1 (MQ=255)                                                                                            cGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTa                                       >  1:914612/1‑140 (MQ=255)                                                                                             gggTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGGCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTAt                                      >  2:675787/1‑140 (MQ=255)                                                                                                     tGCTCTTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCTGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCg                              >  1:223668/1‑140 (MQ=255)                                                                                                       ctctTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGtt                            >  1:267649/1‑140 (MQ=255)                                                                                                         ctTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTcc                                  >  1:505359/1‑132 (MQ=255)                                                                                                         ctTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTcc                                  <  2:505359/132‑1 (MQ=255)                                                                                                         ctTCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGc                          >  1:322881/1‑140 (MQ=255)                                                                                                          ttCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCt                         >  2:485835/1‑140 (MQ=255)                                                                                                           tCAAACAACTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCTGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTc                        <  2:223668/140‑1 (MQ=255)                                                                                                               acaacTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCGGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTcgcgc                    >  2:770222/1‑140 (MQ=255)                                                                                                               acaacTGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTcgcgc                    >  1:584742/1‑140 (MQ=255)                                                                                                                    tGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTgg               >  2:237518/1‑140 (MQ=255)                                                                                                                    tGGGTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTgg               >  2:888950/1‑140 (MQ=255)                                                                                                                     gggTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGc              <  2:584742/140‑1 (MQ=255)                                                                                                                     gggTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGc              <  1:888950/140‑1 (MQ=255)                                                                                                                      ggcataAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTc                        >  1:920944/4‑129 (MQ=255)                                                                                                                      ggTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTc                        <  2:920944/129‑1 (MQ=255)                                                                                                                      ggTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGcc             >  1:341207/1‑140 (MQ=255)                                                                                                                      ggTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGc              <  2:105584/139‑1 (MQ=255)                                                                                                                      ggTATAAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGc              >  1:105584/1‑139 (MQ=255)                                                                                                                          taAGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGAc         <  2:523409/140‑1 (MQ=255)                                                                                                                           aaGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACa        >  2:923126/1‑140 (MQ=255)                                                                                                                            aGGCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACaa       <  1:675787/140‑1 (MQ=255)                                                                                                                             ggCTTGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACaaa      >  2:524352/1‑140 (MQ=255)                                                                                                                                ttGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAacac   >  1:735132/1‑140 (MQ=255)                                                                                                                                 tGCTGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACAcc  <  1:295236/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         ATTGCACGGACTGGCACCGCGCTCAGCAACCATTAAACGGCGGCACTGATCCGGTGAGGGGGTGTAGGTGGTGTCTTTCATCCCCAATGGACGGGTAATCTGCTCTTCAAACAACTGGGTATAAGGCTTGCCGGACGCATTCGCCAGTGCATCTGCCAGCAGGTCGAACGCAAGGTTAGAGTACGCCGCCTGACTGCCTGGCGCGGCTTTCAGCTTCGCCGTAGAAAGGTATTTCCAGCGTTGCTCGCGCGTTGGCCAGACAAACACC  >  NC_000913/395559‑395826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑