Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,960,168	T→C	Y754C (TAC→TGC)	recC ←	exodeoxyribonuclease V subunit RecC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,960,168	0	T	C	100.0%	214.2 / NA	59	Y754C (TAC→TGC)	recC	exodeoxyribonuclease V subunit RecC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/33);  total (26/33)

GTCGTTCGCCTGGCTGGTAGTTTTGTGGATCAAACGGCATCCGGGTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGTAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGATATAGCTGATATAGAGTTTTTGCTGCGCGGAAATTAACGCTTCCAGGAACAGATAGCGGTCGTCATCGCG  >  NC_000913/2960031‑2960304                                                                                                                                          |                                                                                                                                         gTCGTTCGCCTGGCTGGTAGTTTTGTGGATCAAACGGCATCCGGGTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAg                                                                                                                                        >  1:438809/1‑140 (MQ=255)  tCGTTCGCCTGGCTGGTAGTTTTGTGGATCAAACGGCATCCGGGTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGt                                                                                                                                       >  2:30809/1‑140 (MQ=255)  tCGTTCGCCTGGCTGGTAGTTTTGTGGATCAAACGGCATCCGGGTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGt                                                                                                                                       >  2:788935/1‑140 (MQ=255)  tCGTTCGCCTGGCTGGTAGTTTTGTGGATCAAACGGCATCCGGGTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGt                                                                                                                                       <  1:481450/140‑1 (MQ=255)     ttCGCCTGGCTGGTAGTTTTGTGGATCAAACGGCATCCGGGTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGa                                                                                                                                    >  1:588172/1‑140 (MQ=255)      tCGCCTGGCTGGTAGTTTTGTGGATCAAACGGCATCCGGGTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGAt                                                                                                                                   >  1:347924/1‑140 (MQ=255)        gCCTGGCTGGTAGTTTTGTGGATCAAACGGCATCCGGGTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCa                                                                                                                                 <  2:633479/140‑1 (MQ=255)            ggctggTAGTTTTGTGGATCAAACGGCATCCGGGTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTc                                                                                                                             >  1:731011/1‑140 (MQ=255)            ggctggTAGTTTTGTGGATCAAACGGCATCCGGGTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTc                                                                                                                             >  2:81466/1‑140 (MQ=255)             gctggTAGTTTTGTGGATCAAACGGCATCCGGGTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTcc                                                                                                                            <  2:588172/140‑1 (MQ=255)             gctggTAGTTTTGTGGATCAAACGGCATCCGGGGATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTcc                                                                                                                            >  2:962424/1‑140 (MQ=255)                ggTAGTTTTGTGGATCAAACGGCATCCGGGTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGc                                                                                                                         <  2:761187/140‑1 (MQ=255)                  tAGTTTTGTGGATCAAACGGCATCCGGGTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCAc                                                                                                                       <  1:118075/140‑1 (MQ=255)                      tttGTGGATCAAACGGCATCCGGGTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGt                                                                                                                   >  2:763822/1‑140 (MQ=255)                           ggATCAAACGGCATCCGGGTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGa                                                                                                              <  2:438809/140‑1 (MQ=255)                            gATCAAACGGCATCCGGGTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGAc                                                                                                             <  1:579530/140‑1 (MQ=255)                                aaaCGGCATCCGGGTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGa                                                                                                         <  2:731011/140‑1 (MQ=255)                                 aaCGGCATCCGGGTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGaa                                                                                                        >  1:734146/1‑140 (MQ=255)                                      cATCCGGGTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCt                                                                                                   <  1:30809/140‑1 (MQ=255)                                      cATCCGGGTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCt                                                                                                   <  1:269563/140‑1 (MQ=255)                                      cATCCGGGTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCt                                                                                                   <  2:347924/140‑1 (MQ=255)                                       aTCCGGGTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTc                                                                                                  >  1:388722/1‑140 (MQ=255)                                           gggTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTg                                                                                              <  2:1053/140‑1 (MQ=255)                                                   aGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTg                                                                                              <  2:950024/132‑1 (MQ=255)                                                   aGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTg                                                                                              >  1:950024/1‑132 (MQ=255)                                                   aGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTg                                                                                      <  1:962424/140‑1 (MQ=255)                                                      cAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAAt                                                                                   >  2:503994/1‑140 (MQ=255)                                                         gTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGa                                                                                <  1:81466/140‑1 (MQ=255)                                                         gTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGa                                                                                <  2:388722/140‑1 (MQ=255)                                                           aaGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAAc                                                                              <  2:759230/140‑1 (MQ=255)                                                            aGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACg                                                                             <  1:734908/140‑1 (MQ=255)                                                              aTGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGAc                                                                           <  1:151209/140‑1 (MQ=255)                                                               tGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGAcc                                                                          >  1:5314/1‑140 (MQ=255)                                                               tGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGAcc                                                                          >  1:631506/1‑140 (MQ=255)                                                                 cgcTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGa                                                                        >  1:622204/1‑140 (MQ=255)                                                                 cgcTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAAT‑GAACGACCGat                                                                       >  2:236474/1‑140 (MQ=255)                                                                  gcTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGat                                                                       <  2:734146/140‑1 (MQ=255)                                                                  gcTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGat                                                                       <  1:763822/140‑1 (MQ=255)                                                                      ttACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGATATAg                                                                   <  2:5314/140‑1 (MQ=255)                                                                       tACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGATATAGc                                                                  <  2:622204/140‑1 (MQ=255)                                                                             tGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGat                                                                       <  2:453505/129‑1 (MQ=255)                                                                             tGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGat                                                                       >  1:453505/1‑129 (MQ=255)                                                                              gCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGatat                                                                     <  1:636958/130‑1 (MQ=255)                                                                              gCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGatat                                                                     >  2:636958/1‑130 (MQ=255)                                                                                 tCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGATATAGCTGatat                                                            >  1:435954/1‑136 (MQ=255)                                                                                 tCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGATATAGCTGatat                                                            <  2:435954/136‑1 (MQ=255)                                                                                                  gAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGATATAGCTGATATAGAGTTTTTGCTGCGCGGaaa                                       <  1:788935/140‑1 (MQ=255)                                                                                                   aGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGATATAGCTGATATAGAGTTTTTGCTGCGCGGAAAt                                      >  1:397180/1‑140 (MQ=255)                                                                                                   aGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGATATAGCTGATATAGAGTTTTTGCTGCGCGGAAAt                                      >  2:718446/1‑140 (MQ=255)                                                                                                          cATCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGATATAGCTGATATAGAGTTTTTGCTGCGCGGAAATTAACGCt                               >  2:559799/1‑140 (MQ=255)                                                                                                            tCGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGATATAGCTGATATAGAGTTTTTGCTGCGCGGAAATTAACGCTTc                             <  1:120596/140‑1 (MQ=255)                                                                                                             cGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGATATAGCTGATATAGAGTTTTTGCTGCGCGGAAATTAACGCTTcc                            <  2:397180/140‑1 (MQ=255)                                                                                                             cGCCCGGTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAAT‑GAACGACCGATATAGCTGATATAGAGTTTTTGCTGCGCGGAAATTAACGCTTCCa                           <  1:236474/140‑1 (MQ=255)                                                                                                                   gTAGATAATGACTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGATATAGCTGATATAGAGTTTTTGCTGCGCGGAAATTAACGCTTCCAGGAAc                      <  1:559799/140‑1 (MQ=255)                                                                                                                              cTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGATATAGCTGATATAGAGTTTTTGCTGCGCGGAAATTAACGCTTCCa                           <  2:428005/124‑1 (MQ=255)                                                                                                                              cTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGATATAGCTGATATAGAGTTTTTGCTGCGCGGAAATTAACGCTTCCa                           >  1:428005/1‑124 (MQ=255)                                                                                                                              cTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGATATAGCTGATATAGAGTTTTTGCTGCGCGGAAATTAACGCTTCCAGGAACAGATAGCGgtc           >  2:833596/1‑140 (MQ=255)                                                                                                                              cTTTGCCCGATGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGATATAGCTGATATAGAGTTTTTGCTGCGCGGAAATTAACGCTTCCAGGAACAGATAGCGgtc           >  2:274433/1‑140 (MQ=255)                                                                                                                                       aTGCAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGATATAGCTGATATAGAGTTTTTGCTGCGCGGAAATTAACGCTTCCAGGAACAGATAGCGGTCGTCATcgcg  <  1:833596/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GTCGTTCGCCTGGCTGGTAGTTTTGTGGATCAAACGGCATCCGGGTATGGAGGCAAGTAAGATGCGCTTTTACCCTTGCCTCGCTTTCATCACAGTTGAGCGCTTCATCGCCCGGTAGATAATGACTTTGCCCGATGTAGTCGATCAGTTCCTGCACCAGTACCGACGGGAAACGCTCACTGTTATCCTGAATGGAACGACCGATATAGCTGATATAGAGTTTTTGCTGCGCGGAAATTAACGCTTCCAGGAACAGATAGCGGTCGTCATCGCG  >  NC_000913/2960031‑2960304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑