Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,052,840 G→A P227S (CCA→TCA)  ubiH ← 2‑octaprenyl‑6‑methoxyphenol hydroxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,052,8400GA98.0% 171.5 / ‑5.0 50P227S (CCA→TCA) ubiH2‑octaprenyl‑6‑methoxyphenol hydroxylase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (24/25);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTT  >  NC_000913/3052703‑3052971
                                                                                                                                         |                                                                                                                                   
gTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCTAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAAt                                                                                                                                   >  2:604052/1‑140 (MQ=255)
       aaCGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACacca                                                                                                                            <  1:507691/140‑1 (MQ=255)
         cGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACaccaga                                                                                                                          <  1:811432/140‑1 (MQ=255)
         cGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACaccaga                                                                                                                          <  1:960968/140‑1 (MQ=255)
            cAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACaccagacca                                                                                                                       <  1:746983/140‑1 (MQ=255)
             aGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACaccagaccag                                                                                                                      >  2:155375/1‑140 (MQ=255)
               cGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACacca                                                                                                                            >  2:430957/1‑132 (MQ=255)
               cGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACacca                                                                                                                            <  1:430957/132‑1 (MQ=255)
                     aGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACCCCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGc                                                                                                              <  1:405794/140‑1 (MQ=255)
                      gctcTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCAAAAGGCCGGCTGGGGTTCACGGCAAAGCTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGCCAGCAGACCAGCGAACAGcg                                                                                                             <  2:599276/137‑1 (MQ=255)
                           gCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGcgtccgt                                                                                                        >  1:273883/1‑140 (MQ=255)
                            cGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAATCTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGcgtccgtc                                                                                                       >  1:271277/1‑140 (MQ=255)
                            cGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGcgtccgtc                                                                                                       >  2:910005/1‑140 (MQ=255)
                            cGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGcgtccgtc                                                                                                       >  1:686221/1‑140 (MQ=255)
                            cGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGcg                                                                                                             >  1:501743/1‑134 (MQ=255)
                            cGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGcg                                                                                                             <  2:501743/134‑1 (MQ=255)
                              tttACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAg                                                                                                     >  2:385776/1‑140 (MQ=255)
                                tACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGAc                                                                                                   <  1:475174/140‑1 (MQ=255)
                                tACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGAc                                                                                                   <  2:227597/140‑1 (MQ=255)
                                      cGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGc                                                                                             >  2:816193/1‑140 (MQ=255)
                                        tGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCAGGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCaa                                                                                           >  2:431641/1‑140 (MQ=255)
                                           gTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACAt                                                                                        >  1:146805/1‑140 (MQ=255)
                                            tAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATc                                                                                       <  1:752390/140‑1 (MQ=255)
                                                  tCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGc                                                                                 <  1:621709/140‑1 (MQ=255)
                                                     cAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAACCTTCTCGTCACTCCACGACAACCCCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCggg                                                                              <  2:686221/140‑1 (MQ=255)
                                                     cAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCggg                                                                              <  2:355102/140‑1 (MQ=255)
                                                       aGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGcc                                                                            <  2:645909/140‑1 (MQ=255)
                                                       aGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGcc                                                                            <  2:273883/140‑1 (MQ=255)
                                                         tCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCAGGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCAt                                                                          <  1:431641/140‑1 (MQ=255)
                                                            ccagccaAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGtt                                                                       >  2:448801/1‑140 (MQ=255)
                                                                   aaGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTaaa                                                                <  1:155375/140‑1 (MQ=255)
                                                                       ccGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTa                                                                  >  2:98676/1‑134 (MQ=255)
                                                                       ccGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTa                                                                  <  1:98676/134‑1 (MQ=255)
                                                                          aCTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCa                                                         >  1:420780/1‑140 (MQ=255)
                                                                                gTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAgcg                                                   <  2:146805/140‑1 (MQ=255)
                                                                                gTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAgcg                                                   <  1:385776/140‑1 (MQ=255)
                                                                                  tCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAgcgcg                                                 <  1:816193/140‑1 (MQ=255)
                                                                                   cACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAgcgcgc                                                <  2:722189/140‑1 (MQ=255)
                                                                                                   gTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACgg                                <  1:448801/140‑1 (MQ=255)
                                                                                                       cTCCACGACAACACCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGt                            >  2:288092/1‑140 (MQ=255)
                                                                                                               caacaCCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGt                    >  1:886914/1‑140 (MQ=255)
                                                                                                                  cacCTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTgg                 >  1:146746/1‑140 (MQ=255)
                                                                                                                    ccTCTTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCa               >  1:358936/1‑140 (MQ=255)
                                                                                                                     ctctTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCaa              >  2:393974/1‑140 (MQ=255)
                                                                                                                       ctTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATc            >  1:371366/1‑140 (MQ=255)
                                                                                                                       ctTCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCATCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATc            >  1:468458/1‑140 (MQ=255)
                                                                                                                         tCGCGCCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCAc          >  1:709183/1‑140 (MQ=255)
                                                                                                                            cgcCGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGc       <  2:358936/140‑1 (MQ=255)
                                                                                                                               cGTTCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAg    <  2:271277/140‑1 (MQ=255)
                                                                                                                                 ttCCAGTGAATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAgtt  >  1:78268/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                   
GTGGGTTAACGCCAGCGGATAAGCACTGCGTTTACCAGCGTGGGTAATTTTCCCAAGTCGCCAGCCAAAGGCCGACTGGAGTTCACGGCAAAACTTCTCGTCACTCCACGACAACACCTCTTCGCGCCGTTCCAGTGGATGACACCAGACCAGCGAACAGCGTCCGTCAGACATCGGCAACATCGCCAGCGGGCCATGTTGCGTAAAGCGTTCAAAAGCGCGCCCTTCATGCGCAACGGAAGTAGCAACGTTGGCAATCACGGCCAGTT  >  NC_000913/3052703‑3052971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑