Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,180,089	C→T	Q116* (CAA→TAA)	ygiB →	DUF1190 domain‑containing protein YgiB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,180,089	0	C	T	100.0%	198.4 / NA	56	Q116* (CAA→TAA)	ygiB	DUF1190 domain‑containing protein YgiB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/26);  total (30/26)

TGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGT  >  NC_000913/3179953‑3180217                                                                                                                                         |                                                                                                                                 tGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAAt                                                                                                                               >  2:203594/1‑140 (MQ=255) tGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAAt                                                                                                                               >  1:353603/1‑140 (MQ=255)      tGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGc                                                                                                                          >  1:169694/1‑140 (MQ=255)        aaaGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCgg                                                                                                                        >  2:398198/1‑140 (MQ=255)        aaaGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCgg                                                                                                                        >  1:943606/1‑140 (MQ=255)        aaaGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCgg                                                                                                                        <  1:734182/140‑1 (MQ=255)         aagaagCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCggg                                                                                                                       <  1:237650/140‑1 (MQ=255)             agCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATc                                                                                                                              >  2:254123/1‑129 (MQ=255)             agCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATc                                                                                                                              <  1:254123/129‑1 (MQ=255)                    cGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGAt                                                                                                            >  2:197400/1‑140 (MQ=255)                     gTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATg                                                                                                           <  2:353603/140‑1 (MQ=255)                             ccGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGAt                                                                                                   >  2:49038/1‑140 (MQ=255)                               gAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATgg                                                                                                 >  1:508616/1‑140 (MQ=255)                                aaaTACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGc                                                                                                          >  2:736635/1‑130 (MQ=255)                                aaaTACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGc                                                                                                          <  1:736635/130‑1 (MQ=255)                                aaaTACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGc                                                                                                <  2:943606/140‑1 (MQ=255)                                     cGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGtt                                                                                           >  2:69627/1‑140 (MQ=255)                                       ccACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGtt                                                                                           >  1:690545/1‑138 (MQ=255)                                       ccACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGtt                                                                                           <  2:690545/138‑1 (MQ=255)                                          cccGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACatg                                                                                      >  1:63560/1‑140 (MQ=255)                                            cGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACatgat                                                                                    <  1:203594/140‑1 (MQ=255)                                            cGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACatgat                                                                                    <  1:197400/140‑1 (MQ=255)                                                aaGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGACCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATgggg                                                                                >  1:839089/1‑140 (MQ=255)                                                 aGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTTCATGATGGGGc                                                                               <  2:348990/140‑1 (MQ=255)                                                     tgtgTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACatgatg                                                                                   >  1:400175/1‑132 (MQ=255)                                                     tgtgTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACatgatg                                                                                   <  2:400175/132‑1 (MQ=255)                                                       tgtTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGa                                                                         >  2:153277/1‑140 (MQ=255)                                                           gCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATggg                                                                     >  2:897258/1‑140 (MQ=255)                                                           gCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATggg                                                                     >  2:232394/1‑140 (MQ=255)                                                                 tttGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGggcggcgg                                                               >  1:862519/1‑140 (MQ=255)                                                                      tGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCgg                                                          <  2:169694/140‑1 (MQ=255)                                                                         aGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGAt                                                       >  1:585969/1‑140 (MQ=255)                                                                               gTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATTGGCGGCGGCGCGGGATTTGcac                                                 >  1:95264/1‑140 (MQ=255)                                                                                tGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGggcgg                                                                  <  2:612544/122‑1 (MQ=255)                                                                                tGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGggcgg                                                                  >  1:612544/1‑122 (MQ=255)                                                                                tGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGcaca                                                >  2:28652/1‑140 (MQ=255)                                                                                  ccAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCAcagc                                              <  1:69627/140‑1 (MQ=255)                                                                                     gcagGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGGGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACagcagc                                           >  2:159591/1‑140 (MQ=255)                                                                                                cccAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTctcc                                >  1:108239/1‑140 (MQ=255)                                                                                                          catggcaCCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAAcc                      >  2:236814/1‑140 (MQ=255)                                                                                                          catggcaCCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAAcc                      >  1:619859/1‑140 (MQ=255)                                                                                                               caCCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACccagcc                 >  2:217477/1‑140 (MQ=255)                                                                                                               caCCAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACccagcc                 >  1:848077/1‑140 (MQ=255)                                                                                                                  cAGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGt              <  1:606014/140‑1 (MQ=255)                                                                                                                   aGAAAACCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTc             >  2:589628/1‑140 (MQ=255)                                                                                                                       aaCCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGc         <  1:28652/140‑1 (MQ=255)                                                                                                                        aCCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCggat        <  2:862519/140‑3 (MQ=255)                                                                                                                        aCCAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCt        <  2:508616/140‑1 (MQ=255)                                                                                                                         ccAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCtt       <  1:897258/140‑1 (MQ=255)                                                                                                                         ccAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCtt       <  1:217477/140‑1 (MQ=255)                                                                                                                         ccAGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCtt       <  1:232394/140‑1 (MQ=255)                                                                                                                           aGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTAc     <  2:585969/140‑1 (MQ=255)                                                                                                                           aGGCGCAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTAc     <  2:619859/140‑1 (MQ=255)                                                                                                                              cgcAGGCCCAGTAATCCAGCGGGGGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGt  <  1:159591/140‑1 (MQ=255)                                                                                                                              cgcAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGt  <  1:127811/140‑1 (MQ=255)                                                                                                                              cgcAGGCCCAGTAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGt  <  1:398198/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                 TGCGCTGAAAGAAGCCGAACGTACTGCGCCGAAATACGCCACCCGTGAAGACTGTGTTGCTGAATTTGGTGAAGGTCAGTGCCAGCAGGCACCAGCCCAGGCTGGCATGGCACCAGAAAACCAGGCGCAGGCCCAGCAATCCAGCGGGAGTTTCTGGATGCCGCTGATGGCCGGTTACATGATGGGGCGTCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGT  >  NC_000913/3179953‑3180217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑