Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,309,913	T→C	D419G (GAC→GGC)	pnp ←	polynucleotide phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,309,913	0	T	C	100.0%	202.2 / NA	56	D419G (GAC→GGC)	pnp	polynucleotide phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/27);  total (29/27)

TACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGTCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCTACGGAGTACGGAGGGAAGTTGTAGTGGAACAGGAAGGTAT  >  NC_000913/3309779‑3310048                                                                                                                                       |                                                                                                                                        tACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCata                                                                                                                                    >  1:657002/1‑140 (MQ=255)  aCCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCatat                                                                                                                                   >  2:692903/1‑140 (MQ=255)     cGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCg                                                                                                                                <  1:380547/140‑1 (MQ=255)      gCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCgg                                                                                                                               <  1:516568/140‑1 (MQ=255)           ggCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGa                                                                                                                          >  1:460779/1‑140 (MQ=255)                       cGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCAcgc                                                                                                              <  2:874492/140‑1 (MQ=255)                         gCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCAcgccg                                                                                                            <  1:4401/140‑1 (MQ=255)                           acacCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCcgcg                                                                                                          <  2:149671/140‑1 (MQ=255)                           acacCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCcgcg                                                                                                          <  2:114032/140‑1 (MQ=255)                             acCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCt                                                                                                        >  2:11502/1‑140 (MQ=255)                             acCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCt                                                                                                        >  2:109278/1‑140 (MQ=255)                              ccTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCtt                                                                                                       <  1:113601/140‑1 (MQ=255)                              ccTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCtt                                                                                                       <  2:591400/140‑1 (MQ=255)                              ccTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCtt                                                                                                       <  1:702874/140‑1 (MQ=255)                                tGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCg                                                                                                     <  2:775764/140‑1 (MQ=255)                                    tCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAg                                                                                                 >  2:942419/1‑140 (MQ=255)                                       aTCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAgacg                                                                                              >  2:672953/1‑140 (MQ=255)                                        tCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAgacga                                                                                             >  2:515479/1‑140 (MQ=255)                                         cAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAaacgac                                                                                            >  1:624623/1‑140 (MQ=255)                                              ccAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACAGGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGa                                                                                       >  2:64862/1‑140 (MQ=255)                                                       cgccgcACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCa                                                                              <  2:657002/140‑1 (MQ=255)                                                       cgccgcACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCa                                                                              <  1:672953/140‑1 (MQ=255)                                                       cgccgcACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCa                                                                              >  1:858258/1‑140 (MQ=255)                                                            cacaCGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCacgacg                                                                         <  1:692903/140‑1 (MQ=255)                                                                 ggAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCg                                                                    <  2:218718/140‑1 (MQ=255)                                                                  gAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCgg                                                                   >  2:6660/1‑140 (MQ=255)                                                                  gAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAAACGACCGTGACCAATTTCACGACGCTTCgg                                                                   <  2:624623/140‑1 (MQ=255)                                                                             agagGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCa                                                        >  1:764267/1‑140 (MQ=255)                                                                               agGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATg                                                      <  1:942419/140‑1 (MQ=255)                                                                                ggAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGc                                                     >  2:753812/1‑140 (MQ=255)                                                                                           aTTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCt                                          <  1:109278/140‑1 (MQ=255)                                                                                               aGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCtacgg                                      >  1:43058/1‑140 (MQ=255)                                                                                                gTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCtacgga                                     <  1:811312/140‑1 (MQ=255)                                                                                                   aTTTCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCtacggagta                                  <  2:49103/140‑1 (MQ=255)                                                                                                    tttCAGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCtacggagtac                                 >  2:178887/1‑140 (MQ=255)                                                                                                        aGACACAACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCtacggagtacggag                             <  1:515479/140‑1 (MQ=255)                                                                                                           cacaACACGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCTACGGAGTACGGAGGGa                          <  1:6660/140‑1 (MQ=255)                                                                                                             caacaCGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCTACGGAGTACGGAGGGAAg                        >  1:131377/1‑140 (MQ=255)                                                                                                              aacaCGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCTACGGAGTACGGAGGGAAGt                       >  2:190875/1‑140 (MQ=255)                                                                                                              aacaCGTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCTACGGAGTACGGAGGGAAGt                       >  2:324187/1‑140 (MQ=255)                                                                                                                   gTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCTACGGAGTACGGAGGGAAGTTGTAg                  <  2:43058/140‑1 (MQ=255)                                                                                                                   gTACGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCTACGGAGTACGGAGGGAAGTTGTAg                  <  2:843098/140‑1 (MQ=255)                                                                                                                     aCGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCTACGGAGTACGGAGGGAAGTTGTAg                  >  1:797008/1‑138 (MQ=255)                                                                                                                     aCGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCTACGGAGTACGGAGGGAAGTTGTAg                  <  2:797008/138‑1 (MQ=255)                                                                                                                     aCGGTGTACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCTACGGAGTACGGAGGGAAGTTGTAGTg                >  2:844270/1‑140 (MQ=255)                                                                                                                        gtgtACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGTTTTCGCCTACGGAGTACGGAGGGAAGTTGTAGTGGaa             <  2:858258/140‑1 (MQ=255)                                                                                                                        gtgtACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCTACGGAGTACGGAGGGAAGTTGTAGTGGaa             >  1:194231/1‑140 (MQ=255)                                                                                                                         tgtACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCTACGGAGTACGGAGGGAAGTTGTAGTGGAAc            <  1:844270/140‑1 (MQ=255)                                                                                                                          gtACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCTACGGAGTACGGAGGGAAGTTGTAGt                 <  2:295742/134‑1 (MQ=255)                                                                                                                          gtACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCTACGGAGTACGGAGGGAAGTTGTAGt                 >  1:295742/1‑134 (MQ=255)                                                                                                                          gtACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCTACGGAGTACGGAGGGAAGTTGTAGTGGAACa           >  2:464231/1‑140 (MQ=255)                                                                                                                           tACGGGAATTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCTACGGAGTACGGAGGGAAGTTGTAGTGGAACAg          >  1:568159/1‑140 (MQ=255)                                                                                                                                  aTTTGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCTACGGAGTACGGAGGGAAGTTGTAGTGGAACAGGAAGGTa   >  2:580624/1‑140 (MQ=255)                                                                                                                                   tttGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCTACGGAGTACGGAGGGAAGTTGTAGTGGAACAGGAAGGTAt  >  1:315716/1‑140 (MQ=255)                                                                                                                                   tttGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCTACGGAGTACGGAGGGAAGTTGTAGTGGAACAGGAAGGTAt  >  1:387495/1‑140 (MQ=255)                                                                                                                                   tttGCCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCTACGGAGTACGGAGGGAAGTTGTAGTGGAACAGGAAGGTAt  >  2:956648/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        TACCCGCAACGGCAGCTTTGATCGGCACACCTGCGTCCATCAGCGCCAGAGACGCGCCGCACACGGAAGCCATAGAAGAGGAACCGTTGGATTCAGTGATTTCAGACACAACACGTACGGTGTACGGGAATTTGTCCATATCCGGCATGACTGCCAGCACGCCGCGCTTCGCCAGACGACCGTGACCAATTTCACGACGCTTCGGAGAACCGACCATGCCGGTTTCGCCTACGGAGTACGGAGGGAAGTTGTAGTGGAACAGGAAGGTAT  >  NC_000913/3309779‑3310048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑