Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,097,531	(A)8→7	intergenic (+702/+34)	ycdU → / ← serX	uncharacterized protein YcdU/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,524	0	A	.	100.0%	30.2 / NA	13	intergenic (+695/+41)	ycdU/serX	uncharacterized protein YcdU/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (4/9);  total (4/9)

CACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTG  >  NC_000913/1097445‑1097658                                                                                |                                                                                                                                       cACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCg                                                                           <  1:142998/140‑1 (MQ=14)  aCGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGa                                                                          <  1:849889/140‑1 (MQ=17)   cGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGaa                                                                         <  2:312238/140‑1 (MQ=18)                  gCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGa                                                                          >  1:33494/1‑124 (MQ=17)                  gCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGa                                                                          <  2:33494/124‑1 (MQ=17)                    tGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTCGGACCCCCCCTACGGTGCCg                                                        >  1:302435/1‑140 (MQ=11)                      tCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCTGATACGTTGCCGta                                                      <  1:1644512/140‑1 (MQ=255)                          cAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATaca                                                  <  2:443277/140‑1 (MQ=255)                                         ggTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACG‑AAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTg                                   <  1:198006/140‑1 (MQ=255)                                                          cccccGGTGTGTGCAATATACG‑AAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCgg                  <  1:656133/140‑1 (MQ=255)                                                                  gtgtgCAATATACG‑AAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCa          <  1:1563258/140‑1 (MQ=255)                                                                     tgCAATATACG‑AAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGAAACCCCCCCACCTTGCCGTATACACACCTTTCAGGCGGGGTCCTTCCGCCCCCCCGACAcccccccc       >  2:656133/1‑134 (MQ=9)                                                                          tataCG‑AAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGGGTTGAAACCCCCATAACTTGCCCTTTAAACACTTTCCCGGCGGGGTCCTTCCGCCACCCGGACACCCCCCCaaaatt  >  2:934326/1‑137 (MQ=2)                                                                                |                                                                                                                                       CACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTG  >  NC_000913/1097445‑1097658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑