Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,606,330	(G)8→9	noncoding (72/87 nt)	leuQ ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,606,322	1	.	G	89.1%	193.5 / 16.4	64	noncoding (80/87 nt)	leuQ	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (3/4);  new base G (31/26);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01

TTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑G‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTG  >  NC_000913/4606181‑4606451                                                                                                                                               |                                                                                                                                    tttttGATATCGCCTCGTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                                                                                                                                      <  2:588362/140‑1 (MQ=255)  ttttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                                                                                                                                      <  1:89476/140‑1 (MQ=255)   tttGATATCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑g                                                                                                                                    <  2:1420971/140‑1 (MQ=255)       atatCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                                                                                                                                      <  1:1172736/135‑1 (MQ=255)       atatCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa                                                                                                                                      >  2:1172736/1‑135 (MQ=255)        tatCGCCTCGTTTGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGGGA‑g‑ggggg                                                                                                                              <  1:174946/140‑1 (MQ=255)           cGCCTCGTTTGGTGCGA‑GGGGGGGNCNTGNACCCCCACGTCCGTAAGAACACTNACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTNCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑g‑gggggggg                                                                                                                           <  2:205096/140‑2 (MQ=255)                  tttGGTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGCGCGAGCAATAAAAAAGTGGTTTTTCGACAGA‑G‑GGGGGGGCACTTGaa                                                                                                                    >  2:256084/1‑140 (MQ=17)                      gTGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAAcccc                                                                                                                <  1:1629200/140‑1 (MQ=255)                       tGCGA‑GGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑G‑GGGGGGG‑ACTTGAACCCCCa                                                                                                              <  2:672559/140‑1 (MQ=255)                          cgagggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCAc                                                                                                             <  1:1151286/137‑1 (MQ=255)                          agggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCAc                                                                                                             <  1:1043362/138‑1 (MQ=255)                            agggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCa                                                                                                              >  1:1501218/2‑137 (MQ=255)                            agggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCa                                                                                                              <  2:1501218/136‑1 (MQ=255)                            agggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGt                                                                                                           >  1:1390180/2‑140 (MQ=255)                            agggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGt                                                                                                           >  1:393418/2‑140 (MQ=255)                             gggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTc                                                                                                          <  2:130405/140‑1 (MQ=255)                             gggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑G‑GGGGGGG‑ACtt                                                                                                                       <  2:642375/126‑1 (MQ=255)                             gggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑G‑GGGGGGG‑ACtt                                                                                                                       >  1:642375/1‑126 (MQ=255)                              ggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGAGGTTTTTGGTGCGAGG‑GGGGGGG‑TCTTGAACCCCTACGTcc                                                                                                         >  2:1562030/1‑140 (MQ=21)                                  ggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCg                                                                                                        >  1:1288211/1‑137 (MQ=255)                                  ggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCg                                                                                                        <  2:1288211/137‑1 (MQ=255)                                  ggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTaa                                                                                                     >  1:282023/1‑140 (MQ=255)                                    aCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAgg                                                                                                   <  1:439124/140‑1 (MQ=255)                                     ttttaaCCGCCCCGTATGTAAGGACACTAACCCCTGAAGCTGGCGTGTCTACCAAGTCCGCCACCTACGCCCAGTGCGAGAAAGAACAAAGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGa                                                                                                  <  2:1447722/136‑1 (MQ=21)                                       tGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGaca                                                                                                <  2:393418/140‑1 (MQ=255)                                          aCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTa                                                                                             <  2:282023/140‑1 (MQ=255)                                           cccccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                            <  2:1584416/140‑1 (MQ=255)                                           cccccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑GCGGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                            <  2:329667/140‑1 (MQ=255)                                                   tCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGaa                                                                                    >  1:1584416/1‑140 (MQ=255)                                                            acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                            <  2:516834/123‑1 (MQ=255)                                                            acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                            >  1:516834/1‑123 (MQ=255)                                                            acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGcgcg                                                                           <  2:1262822/140‑1 (MQ=255)                                                             cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGt                                                                          <  2:1252833/140‑1 (MQ=255)                                                                tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCt                                                                                       <  2:1585107/124‑1 (MQ=255)                                                                tAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCt                                                                                       >  1:1585107/1‑124 (MQ=255)                                                                 aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAc                                                                      >  1:708279/1‑140 (MQ=255)                                                                  acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAcc                                                                     >  2:1512444/1‑140 (MQ=255)                                                                     ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                                                  >  1:1252833/1‑140 (MQ=255)                                                                        gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                               >  1:1285157/1‑140 (MQ=255)                                                                        gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                               >  1:286606/1‑140 (MQ=255)                                                                              aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                         >  1:898779/1‑140 (MQ=255)                                                                              aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                         >  2:990850/1‑140 (MQ=255)                                                                              aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAA‑CCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                                                        >  1:1002773/1‑140 (MQ=255)                                                                                cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                                                       >  2:177487/1‑140 (MQ=255)                                                                                cgcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                                                       >  1:1218536/1‑140 (MQ=255)                                                                                       aCCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATAc                                                <  1:214515/140‑1 (MQ=255)                                                                                             tCCGCCACCCTCGCCCAGTTCGATTAATATCATCGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCaa                                          <  2:997937/140‑1 (MQ=18)                                                                                               cGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAAtt                                        >  2:310443/1‑140 (MQ=255)                                                                                                gCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTc                                       >  1:1441507/1‑140 (MQ=255)                                                                                                 ccACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTAGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa                                                   >  1:323240/1‑127 (MQ=255)                                                                                                 ccACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTAGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCa                                                   <  2:323240/127‑1 (MQ=255)                                                                                                  cACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCtt                                     >  2:447236/1‑140 (MQ=255)                                                                                                  cACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCtt                                     <  2:1285157/140‑1 (MQ=255)                                                                                                    ccTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaa                                   >  2:1452737/1‑140 (MQ=255)                                                                                                       tCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaaa                                >  2:1240825/1‑140 (MQ=255)                                                                                                              gTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGc                         >  2:252631/1‑140 (MQ=255)                                                                                                                gCGAGCAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTa                       >  1:32622/1‑140 (MQ=255)                                                                                                                     cAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg                  <  1:1512444/140‑1 (MQ=255)                                                                                                                     cAATATCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg                  <  2:708279/140‑1 (MQ=255)                                                                                                                        tatCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg               <  1:1452737/140‑1 (MQ=255)                                                                                                                         atCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAAt                            <  1:1410300/126‑1 (MQ=255)                                                                                                                         atCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAAt                            >  2:1410300/1‑126 (MQ=255)                                                                                                                         atCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACgg                 <  1:911692/137‑1 (MQ=255)                                                                                                                         atCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACgg                 >  2:911692/1‑137 (MQ=255)                                                                                                                         atCAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAgg              >  1:698341/1‑140 (MQ=255)                                                                                                                         atCAACGTGGTTTTTGGTGCGA‑G‑GGGGGGG‑GCTTGGACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCCCCTTCGCATACCATCAATTCTTAAAAAGAAATGCTACCACGGAGgc             >  2:1086600/1‑140 (MQ=255)                                                                                                                           cAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg            <  1:990850/140‑1 (MQ=255)                                                                                                                           cAACGTGGTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg            <  1:1562030/140‑1 (MQ=255)                                                                                                                                tGGTTTTTGGTGCGA‑G‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg                  >  1:787968/1‑128 (MQ=255)                                                                                                                                tGGTTTTTGGTGCGA‑G‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg                  <  2:787968/128‑1 (MQ=255)                                                                                                                                  gTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTa     >  1:182021/1‑140 (MQ=255)                                                                                                                                  gTTTTTGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTa     >  2:357062/1‑140 (MQ=255)                                                                                                                                     tttGGTGCGAGG‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTg  <  2:1441507/140‑1 (MQ=255)                                                                                                                                               |                                                                                                                                    TTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑G‑GGGGGGG‑ACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTG  >  NC_000913/4606181‑4606451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑