Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	427,568	A→G	K94K (AAA→AAG)	yajC →	Sec translocon accessory complex subunit YajC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	427,568	0	A	G	100.0%	273.6 / NA	76	K94K (AAA→AAG)	yajC	Sec translocon accessory complex subunit YajC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/37);  total (39/37)

ACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGTCAT  >  NC_000913/427432‑427702                                                                                                                                         |                                                                                                                                       acaAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGt                                                                                                                                     <  2:1542408/140‑1 (MQ=255)   aaaaaGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGa                                                                                                                                   <  2:733808/140‑1 (MQ=255)     aaaGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACt                                                                                                                                 >  1:1096936/1‑140 (MQ=255)     aaaGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACt                                                                                                                                 >  1:369632/1‑140 (MQ=255)        gCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCg                                                                                                                              >  1:1237917/1‑140 (MQ=255)             tGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCt                                                                                                                         >  2:1018484/1‑140 (MQ=255)                 cTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCg                                                                                                                     <  1:499079/140‑1 (MQ=255)                    cATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTcc                                                                                                                  <  1:1018484/140‑1 (MQ=255)                      ttGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTg                                                                                                                >  2:799861/1‑140 (MQ=255)                            aaGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGaaa                                                                                                          <  2:883107/140‑1 (MQ=255)                            aaGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGaaa                                                                                                          <  1:407627/140‑1 (MQ=255)                            aaGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGaaa                                                                                                          <  2:1338231/140‑1 (MQ=255)                              ggTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAgg                                                                                                        <  1:682537/140‑1 (MQ=255)                              ggTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAgg                                                                                                        >  1:746906/1‑140 (MQ=255)                              ggTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAgg                                                                                                        <  1:806178/140‑1 (MQ=255)                              ggTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAgg                                                                                                        <  2:1096936/140‑1 (MQ=255)                              ggTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAgg                                                                                                        >  1:1112889/1‑140 (MQ=255)                                     aaGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATg                                                                                                 <  1:458508/140‑1 (MQ=255)                                        ttCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAg                                                                                              >  1:200032/1‑140 (MQ=255)                                         tCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAgg                                                                                             >  1:1643606/1‑140 (MQ=255)                                         tCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAgg                                                                                             >  2:141867/1‑140 (MQ=255)                                         tCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCAAGAAgg                                                                                             >  2:1535414/1‑140 (MQ=255)                                             aCGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCt                                                                                         <  2:1112889/140‑1 (MQ=255)                                             aCGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCt                                                                                         >  1:351472/1‑140 (MQ=255)                                             aCGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCt                                                                                         >  2:36682/1‑140 (MQ=255)                                                  cGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAAt                                                                                    >  1:427082/1‑140 (MQ=255)                                                    gTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTa                                                                                  <  1:36682/140‑1 (MQ=255)                                                       gCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGa                                                                                                <  2:1432453/123‑1 (MQ=255)                                                       gCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTaaaa                                                                               >  1:1293302/1‑140 (MQ=255)                                                       gCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCCGCCGTCCTGCCGAAAGGCACCATGa                                                                                                >  1:1432453/1‑123 (MQ=255)                                                                 tCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTa                                                                     >  2:1159019/1‑140 (MQ=255)                                                                  cgcgTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTaa                                                                    >  1:974384/1‑140 (MQ=255)                                                                      tAACCAAAGTAGCGGAAAACGACTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGa                                                                >  1:485582/1‑140 (MQ=255)                                                                       aaCCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGaa                                                               <  2:746906/140‑1 (MQ=255)                                                                                 gCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGtt                                                     >  1:1382401/1‑140 (MQ=255)                                                                                 gCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGtt                                                     >  1:1533765/1‑140 (MQ=255)                                                                                  cGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTa                                                    <  1:799861/140‑1 (MQ=255)                                                                                  cGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTa                                                    <  2:200032/140‑1 (MQ=255)                                                                                    gAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTaaa                                                  >  1:1176373/1‑140 (MQ=255)                                                                                          ggCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTa                                            <  1:1159019/140‑1 (MQ=255)                                                                                          ggCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTa                                            <  2:378992/140‑1 (MQ=255)                                                                                          ggCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTa                                            <  1:18960/140‑1 (MQ=255)                                                                                            cTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATc                                          <  2:958509/140‑1 (MQ=255)                                                                                            cTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATc                                          <  2:351472/140‑1 (MQ=255)                                                                                              aCATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCt                                        <  2:974384/140‑1 (MQ=255)                                                                                              aCATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCt                                        <  2:1293302/140‑1 (MQ=255)                                                                                               cATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCtt                                       >  2:413169/1‑140 (MQ=255)                                                                                                 ttGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTtg                                     <  2:1317194/140‑1 (MQ=255)                                                                                                        cgcgCTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCAAGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGt                              <  1:1535414/140‑1 (MQ=255)                                                                                                         gcgcTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGaa                                <  2:722571/137‑1 (MQ=255)                                                                                                         gcgcTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGaa                                >  1:722571/1‑137 (MQ=255)                                                                                                          cgcTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATc                                          <  1:368359/126‑1 (MQ=255)                                                                                                          cgcTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATc                                          >  2:368359/1‑126 (MQ=255)                                                                                                          cgcTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTAc                            >  1:601797/1‑140 (MQ=255)                                                                                                           gcTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACg                           <  2:315011/140‑1 (MQ=255)                                                                                                           gcTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACg                           <  2:485582/140‑1 (MQ=255)                                                                                                            cTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGt                          >  2:1431487/1‑140 (MQ=255)                                                                                                            cTGAATGACACCACTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGt                          >  2:362191/1‑140 (MQ=255)                                                                                                                       cacTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATg                      >  2:568458/1‑133 (MQ=255)                                                                                                                       cacTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATg                      <  1:568458/133‑1 (MQ=255)                                                                                                                         cTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGt                              >  1:1365566/1‑123 (MQ=255)                                                                                                                         cTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGt                              <  2:1365566/123‑1 (MQ=255)                                                                                                                         cTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGt                          <  2:95041/127‑1 (MQ=255)                                                                                                                         cTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGt                          >  1:95041/1‑127 (MQ=255)                                                                                                                         cTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCt                    <  2:1375470/133‑1 (MQ=255)                                                                                                                         cTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCt                    >  1:1375470/1‑133 (MQ=255)                                                                                                                         cTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCgtg             >  1:1224394/1‑140 (MQ=255)                                                                                                                         cTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCgtg             >  2:725039/1‑140 (MQ=255)                                                                                                                         cTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCgtg             >  1:629027/1‑140 (MQ=255)                                                                                                                         cTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCgtg             >  2:641604/1‑140 (MQ=255)                                                                                                                         cTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCgtg             <  2:427082/140‑1 (MQ=255)                                                                                                                         cTGAAGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCgct             <  2:1237917/140‑3 (MQ=255)                                                                                                                            aaGTAGTTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCgtggtg          <  1:1431487/140‑1 (MQ=255)                                                                                                                                 gtTATTAAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGt     >  1:1652121/1‑140 (MQ=255)                                                                                                                                    attaAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCgtg             >  2:47727/1‑129 (MQ=255)                                                                                                                                    attaAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCgtg             <  1:47727/129‑1 (MQ=255)                                                                                                                                    attaAGCGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGTCAt  >  2:357/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       ACAAAAAGCTGATGGACTCCATTGCCAAAGGTGATGAAGTTCTGACGAACGGTGGCCTGGTTGGTCGCGTAACCAAAGTAGCGGAAAACGGCTACATTGCTATCGCGCTGAATGACACCACTGAAGTAGTTATTAAACGTGACTTCGTAGCTGCCGTCCTGCCGAAAGGCACCATGAAGGCGCTGTAATTAAAATTTTTCCCTAAGGGAATTGCCGTGTTAAACCGTTATCCTTTGTGGAAGTACGTCATGCTGATCGTGGTGATTGTCAT  >  NC_000913/427432‑427702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑