Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,895,366	T→C	Q138Q (CAA→CAG)	ygcQ ←	putative flavoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,895,366	0	T	C	100.0%	213.0 / NA	61	Q138Q (CAA→CAG)	ygcQ	putative flavoprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/26);  total (35/26)

TCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGTTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTCCCCAGTGGGATTTGCGCACGCTTACGGTTG  >  NC_000913/2895229‑2895502                                                                                                                                          |                                                                                                                                         tCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGttgctg                                                                                                                                        >  1:1168371/1‑140 (MQ=255) tCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGttgctg                                                                                                                                        >  1:1501207/1‑140 (MQ=255) tCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGttgctg                                                                                                                                        >  1:1481603/1‑140 (MQ=255)   cTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCa                                                                                                                                      <  1:1660172/140‑1 (MQ=255)   cTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCa                                                                                                                                      <  1:1498422/140‑1 (MQ=255)   cTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCa                                                                                                                                      <  2:937317/140‑1 (MQ=255)   cTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCa                                                                                                                                      <  2:625096/140‑1 (MQ=255)                tctcCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCaa                                                                                                                         >  2:459351/1‑140 (MQ=255)                 ctcCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAAc                                                                                                                        >  1:1086247/1‑140 (MQ=255)                 ctcCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAAc                                                                                                                        >  1:1395785/1‑140 (MQ=255)                  tcCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCa                                                                                                                                 >  1:1619431/1‑130 (MQ=255)                  tcCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCa                                                                                                                                 <  2:1619431/130‑1 (MQ=255)                           ccccACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGtttt                                                                                                              >  2:889838/1‑140 (MQ=255)                           ccccACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGtttt                                                                                                              >  1:1246486/1‑140 (MQ=255)                           ccccACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGtttt                                                                                                              >  1:445512/1‑140 (MQ=255)                              caccaccaGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCg                                                                                                           <  2:360406/140‑1 (MQ=255)                              caccaccaGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCg                                                                                                           <  2:1246486/140‑1 (MQ=255)                                ccaccaGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCgct                                                                                                         >  2:381336/1‑140 (MQ=255)                                ccaccaGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGCAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCgct                                                                                                         >  2:271197/1‑140 (MQ=255)                                 caccaGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCgctg                                                                                                        <  2:1481603/140‑1 (MQ=255)                                     aGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTcc                                                                                                    >  1:860955/1‑140 (MQ=255)                                           cGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTg                                                                                              >  2:1399747/1‑140 (MQ=255)                                            gTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGa                                                                                             <  2:1086247/140‑1 (MQ=255)                                            gTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCCAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGa                                                                                             <  1:253011/140‑1 (MQ=255)                                            ctCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGa                                                                                             <  1:286309/139‑1 (MQ=255)                                             tCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGAc                                                                                            >  1:619342/1‑140 (MQ=255)                                                   cTCAGCTAATGGGGCGCGGGTAACATTTTTCAGAGCCTCTGTGCTAACCAGCCAGGCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCa                                                                                      <  2:1348993/140‑1 (MQ=255)                                                           aTGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAgg                                                                              >  1:203769/1‑140 (MQ=255)                                                           aTGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAgg                                                                              <  1:560949/140‑1 (MQ=255)                                                                tCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGgaactggcct                                                                         >  1:977826/1‑133 (MQ=255)                                                                tCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAg                                                                         >  2:1157343/1‑140 (MQ=255)                                                                tCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAg                                                                         >  1:635351/1‑140 (MQ=255)                                                                   cgGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCgg                                                                      >  1:1510685/1‑140 (MQ=255)                                                                           aTTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGttttt                                                              <  2:1536253/140‑1 (MQ=255)                                                                               ttCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGt                                                          >  2:965609/1‑140 (MQ=255)                                                                                  aGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTg                                                       <  1:271197/140‑1 (MQ=255)                                                                                       ctctGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCg                                                  <  1:1157343/140‑1 (MQ=255)                                                                                         ctGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTc                                                >  1:1476221/1‑140 (MQ=255)                                                                                                 aCCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAg                                        >  2:1281585/1‑140 (MQ=255)                                                                                                 aCCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCG‑TTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAgc                                       >  1:1249953/1‑140 (MQ=255)                                                                                                   cagccagTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAgcg                                      >  1:215355/1‑140 (MQ=255)                                                                                                      ccagTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCAt                                   >  2:917784/1‑140 (MQ=255)                                                                                                         gTCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTc                                >  1:209985/1‑140 (MQ=255)                                                                                                          tCCGGAAGCGCACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTcc                               >  2:1644563/1‑140 (MQ=255)                                                                                                                    cACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCAt                                   >  1:1444981/1‑126 (MQ=255)                                                                                                                    cACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCAt                                   <  2:282586/126‑1 (MQ=255)                                                                                                                    cACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCAt                                   <  2:1444981/126‑1 (MQ=255)                                                                                                                    cACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCAt                                   >  1:282586/1‑126 (MQ=255)                                                                                                                    cACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTCCCCAGTGGGAt                     >  1:942431/1‑140 (MQ=255)                                                                                                                    cACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTCCCCAGTGGGAt                     >  2:881151/1‑140 (MQ=255)                                                                                                                    cACCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTCCCCAGTGGGAt                     >  1:520974/1‑140 (MQ=255)                                                                                                                     aCCCGGGACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTCCCCAGTGGGAtt                    <  2:520974/140‑1 (MQ=255)                                                                                                                         gggACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTCCCCAGTGGGATTTgcg                <  1:2865/140‑1 (MQ=255)                                                                                                                         gggACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTCCCCAGTGGGATTTgcg                <  1:889838/140‑1 (MQ=255)                                                                                                                         gggACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTCCCCAGTGGGATTTgcg                <  2:635351/140‑1 (MQ=255)                                                                                                                         gggACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTCCCCAGTGGGATTTgcg                <  2:619342/140‑1 (MQ=255)                                                                                                                          ggACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTCCCCAGTGGGATTTgcgc               >  1:923852/1‑140 (MQ=255)                                                                                                                           gACAATGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTCCCCAGTGGGATTTGCGCa              >  1:1232407/1‑140 (MQ=255)                                                                                                                               aTGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTCCCCAGTGGGATTTGCGCACGCt          <  1:550330/140‑1 (MQ=255)                                                                                                                               aTGTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTCCCCAGTGGGATTTGCGCACGCt          >  1:518585/1‑140 (MQ=255)                                                                                                                                 gTTCAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTCCCCAGTGGGATTTGCGCACGCTTa        >  1:514300/1‑140 (MQ=255)                                                                                                                                    cAGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTCCCCAGTGGGATTTGCGCACGCTTACgg     <  2:445512/140‑1 (MQ=255)                                                                                                                                     aGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTCCCCAGTGGGATTTGCGCACGCTTACgg     >  2:1449825/1‑139 (MQ=255)                                                                                                                                     aGTTGCTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGCGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTCCCCAGTGGGATTTGCGCACGCTTACgg     <  1:1449825/139‑1 (MQ=255)                                                                                                                                      gttgctGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTCCCCAGTGGGATTTgcg                >  1:1447646/1‑127 (MQ=255)                                                                                                                                      gttgctGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTCCCCAGTGGGATTTgcg                <  2:1447646/127‑1 (MQ=255)                                                                                                                                      gttgctGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTCCCCAGTGGGATTTGCGCACGCTTACGGt    <  2:857766/139‑1 (MQ=255)                                                                                                                                      gttgctGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTCCCCAGTGGGATTTGCGCACGCTTACGGt    >  1:857766/1‑139 (MQ=255)                                                                                                                                       ttgctgCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTCCCCAGTGGGATTTGCGCACGCTTACGGTTg  <  1:1261524/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         TCCTGGTTATCTGCCTCTCCGCCCTGCCCCACCACCAGCACCCGTCTGGCCTCAGCTAATGGGTCGCGGGTAACATTTTTCAGATCCTCTGTGCTAACCAGCCAGTCCGGAAGCGCACCCGGGACAATGTTCAGTTGTTGCATTCCAGAGGGCAACGTGGCGTTTTTCGCTGCTCCCGCCTGACGCGCCAGGGAAAGGCATAGCGGGCGTTTTTCTGTTTGCAGCGTCGCCGTTAGCGCATTTCCCCAGTGGGATTTGCGCACGCTTACGGTTG  >  NC_000913/2895229‑2895502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑