Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,030,784	C→T	A50T (GCT→ACT)	ygfT ←	fused putative oxidoreductase, Fe‑S subunit and nucleotide‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,030,784	0	C	T	100.0%	323.6 / NA	92	A50T (GCT→ACT)	ygfT	fused putative oxidoreductase, Fe‑S subunit and nucleotide‑binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (51/41);  total (51/41)

TTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATA  >  NC_000913/3030649‑3030920                                                                                                                                        |                                                                                                                                         ttGCGCTTTTTTTACACCAAATACTTTTTTGCTCGCCCAGTTGTCCGCTATGGGACGGAAAAGTCAGAGAATTACCCGGCCAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTgg                                                                                                                                      <  2:359590/140‑1 (MQ=255) tggcgcATTTTTTACAACTAATATTTTTTTCTGCGTCCATTTGACCGCTGCGGCGCAGAAACGTCAGAGCATTAACCGACCAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTgg                                                                                                                                      <  2:763105/138‑1 (MQ=255)         ttttaACAACAAATACATTTTTCCTCGTCCAGTTGTCCGCTATCGGACTGGAAAGTCAGAGCATTACCCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTccc                                                                                                                              <  2:1028528/139‑1 (MQ=255)          ttttACAACCAATACTTTTTTGCTCGTCCATTTGTCCGCTATCGGACTGGAAAGTCAGAGCATTACCCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCt                                                                                                                             <  2:770711/140‑1 (MQ=255)              aCAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACaa                                                                                                                         >  1:1028528/1‑140 (MQ=255)                   cAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTgg                                                                                                                    >  1:1393172/1‑140 (MQ=255)                    aaTACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGa                                                                                                                   >  2:724470/1‑140 (MQ=255)                     aTACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGAt                                                                                                                  >  2:12108/1‑140 (MQ=255)                      tACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGCCAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGt                                                                                                                      <  1:1541679/135‑1 (MQ=255)                      tACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGt                                                                                                                      >  2:1541679/1‑135 (MQ=255)                      tACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATa                                                                                                                 >  2:597823/1‑140 (MQ=255)                              tGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCg                                                                                                         >  1:81160/1‑140 (MQ=255)                                cTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTGCCTACAACGTGGATACGCGGTCGaa                                                                                                       >  2:63259/1‑140 (MQ=255)                                cTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGaa                                                                                                       >  2:844829/1‑140 (MQ=255)                                 tCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGaaa                                                                                                      <  2:1468295/140‑1 (MQ=255)                                   gTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTACCCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGt                                                                                                    <  2:1393172/140‑1 (MQ=255)                                   gTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGt                                                                                                    >  1:1609238/1‑140 (MQ=255)                                     ccAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCa                                                                                                  >  1:1483472/1‑140 (MQ=255)                                              cGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTc                                                                                         >  2:1020122/1‑140 (MQ=255)                                              cGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTc                                                                                         >  2:887701/1‑140 (MQ=255)                                              cGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTc                                                                                         >  1:1204570/1‑140 (MQ=255)                                                      gACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCa                                                                                 >  2:1016009/1‑140 (MQ=255)                                                      gACTGGAAAGCCAGAGCTTAAACCGCACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCa                                                                                 <  2:1204570/140‑1 (MQ=255)                                                      gACGGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGTTACGCGGTCGAAAGTCACTGTGACTCAGCGGCCa                                                                                 <  1:33718/140‑1 (MQ=255)                                                        cTGGAAAGTCAGAGCATTACCCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGt                                                                               <  2:1001413/140‑1 (MQ=255)                                                        cTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGt                                                                               <  1:1020122/140‑1 (MQ=255)                                                         tGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGtt                                                                              >  1:833012/1‑140 (MQ=255)                                                           gAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGtttt                                                                            >  1:1256241/1‑140 (MQ=255)                                                                tCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGc                                                                                   <  1:1021870/128‑1 (MQ=255)                                                                tCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGc                                                                                   >  2:1021870/1‑128 (MQ=255)                                                                tCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGa                                                                       <  2:81160/140‑1 (MQ=255)                                                                tCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCAAGTTTTCTTGa                                                                       <  2:1609238/140‑1 (MQ=255)                                                                 cAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGa                                                                       >  1:426840/1‑139 (MQ=255)                                                                 cAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGa                                                                       <  2:426840/139‑1 (MQ=255)                                                                    agCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGa                                                                       <  1:330311/136‑1 (MQ=255)                                                                    agCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGa                                                                       >  2:330311/1‑136 (MQ=255)                                                                    agCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATtgt                                                                   >  2:1186253/1‑140 (MQ=255)                                                                     gCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATtgtg                                                                  >  1:1464455/1‑140 (MQ=255)                                                                     gCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATtgtg                                                                  >  2:734653/1‑140 (MQ=255)                                                                         tAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGcc                                                              >  2:659244/1‑140 (MQ=255)                                                                              ggACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGc                                                         >  2:41188/1‑140 (MQ=255)                                                                               gataaGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGca                                                        <  1:956753/137‑1 (MQ=255)                                                                               gACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGca                                                        <  1:1186253/140‑1 (MQ=255)                                                                               gACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGAGGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGTCTCAGCGGCCAGTTTTCTTGATTGTGTGcttgcgca                                                        <  2:1049640/140‑8 (MQ=255)                                                                                aCAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGcac                                                       >  1:714402/1‑140 (MQ=255)                                                                                aCAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGcac                                                       >  2:1262029/1‑140 (MQ=255)                                                                                aCAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCATACAACGTGGATACGCGGTCGAAAATCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGcac                                                       >  2:152932/1‑140 (MQ=255)                                                                                 cAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGcaca                                                      <  1:659244/140‑1 (MQ=255)                                                                                  aaGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAg                                                     <  1:1302127/140‑1 (MQ=255)                                                                                    gCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGc                                                   >  1:153084/1‑140 (MQ=255)                                                                                    gCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGc                                                   >  2:298798/1‑140 (MQ=255)                                                                                    gCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGc                                                   <  2:714402/140‑1 (MQ=255)                                                                                    gCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGc                                                   >  2:1487981/1‑140 (MQ=255)                                                                                     ccGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCa                                                  >  2:1506744/1‑140 (MQ=255)                                                                                          aCGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTc                                             >  2:1341317/1‑140 (MQ=255)                                                                                               aGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAg                                        <  2:153084/140‑1 (MQ=255)                                                                                                ggggCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGc                                       >  1:6454/1‑140 (MQ=255)                                                                                                ggggCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGc                                       >  2:435575/1‑140 (MQ=255)                                                                                                   gCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATg                                    <  1:734653/140‑1 (MQ=255)                                                                                                   gCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATg                                    <  1:666677/140‑1 (MQ=255)                                                                                                   gCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATg                                    <  1:1341317/140‑1 (MQ=255)                                                                                                   gCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATg                                    <  1:1016009/140‑1 (MQ=255)                                                                                                    cATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGa                                   <  1:278914/140‑1 (MQ=255)                                                                                                    cATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGa                                   <  1:12108/140‑1 (MQ=255)                                                                                                     aTTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGAc                                  >  2:738146/1‑140 (MQ=255)                                                                                                     aTTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGAc                                  <  1:597823/140‑1 (MQ=255)                                                                                                      ttgttgCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACa                                 >  1:338519/1‑140 (MQ=255)                                                                                                      ttgttgCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACa                                 >  1:669632/1‑140 (MQ=255)                                                                                                          tgCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCt                             <  1:361032/140‑1 (MQ=255)                                                                                                           gCAGTGATGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCta                            >  1:1440377/1‑140 (MQ=255)                                                                                                                 aTGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCa                                      <  1:786947/124‑1 (MQ=255)                                                                                                                 aTGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCa                                      >  2:786947/1‑124 (MQ=255)                                                                                                                 aTGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATg                                    >  2:1361667/1‑126 (MQ=255)                                                                                                                 aTGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATg                                    <  1:1361667/126‑1 (MQ=255)                                                                                                                 aTGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACAtt                      >  1:510718/1‑140 (MQ=255)                                                                                                                 aTGGCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACAtt                      >  2:1087042/1‑140 (MQ=255)                                                                                                                   ggCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTcc                    <  2:125232/140‑1 (MQ=255)                                                                                                                   ggCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTcc                    >  2:925422/1‑140 (MQ=255)                                                                                                                   ggCAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTcc                    >  2:736298/1‑140 (MQ=255)                                                                                                                     cAGGCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGc                  <  1:887701/140‑1 (MQ=255)                                                                                                                     cAGGCCACCGGATTCGCAGTCTGGCCTTTCCATACAACGTGGATACGCGGTCGAAAATCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGc                  <  1:152932/140‑1 (MQ=255)                                                                                                                       ggCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAg                >  1:629521/1‑140 (MQ=255)                                                                                                                        gCCACCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGc               >  2:961829/1‑140 (MQ=255)                                                                                                                           aCCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTc            >  2:79101/1‑140 (MQ=255)                                                                                                                           aCCGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTc            >  1:358267/1‑140 (MQ=255)                                                                                                                             cGGATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTcag          >  1:1084471/1‑140 (MQ=255)                                                                                                                              ggATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTcagc         <  2:1440377/140‑1 (MQ=255)                                                                                                                              ggATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTcagc         <  2:833012/140‑1 (MQ=255)                                                                                                                              ggATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTcagc         <  1:1262029/140‑1 (MQ=255)                                                                                                                               gATTCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTcagca        <  2:1464455/140‑1 (MQ=255)                                                                                                                                  tCGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAgcagca     >  1:1019594/1‑140 (MQ=255)                                                                                                                                   cGCAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCaa    <  2:1019594/140‑1 (MQ=255)                                                                                                                                     cAGTCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATa  <  2:338519/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         TTGCGCATCTTTTACAACCAATACATTTTTGCTCGTCCAGTTGTACGCTATCGGACTGGAAAGTCAGAGCATTAACCGGACAAGCCGTAACGCAAGGGGCATTGTTGCAGTGATGGCAGGCCACCGGATTCGCAGCCTGGCCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATA  >  NC_000913/3030649‑3030920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑