Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,123,295	G→A	R171W (CGG→TGG)	glcB ←	malate synthase G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,123,295	0	G	A	100.0%	348.7 / NA	100	R171W (CGG→TGG)	glcB	malate synthase G
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (49/51);  total (49/51)

ACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTC  >  NC_000913/3123160‑3123422                                                                                                                                        |                                                                                                                                aCTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAACCGCCACCCCATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAAcc                                                                                                                             <  2:982205/140‑1 (MQ=255) aCTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAAcc                                                                                                                             >  2:135249/1‑140 (MQ=255)  cTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAAccc                                                                                                                            <  1:44779/140‑1 (MQ=255)  cTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAAccc                                                                                                                            <  1:1585738/140‑1 (MQ=255)   tgtgCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCa                                                                                                                           >  1:552538/1‑140 (MQ=255)     tgCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAgg                                                                                                                         >  1:931472/1‑140 (MQ=255)      gCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGc                                                                                                                        >  1:1480010/1‑140 (MQ=255)          gAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAgg                                                                                                                         >  1:468701/1‑135 (MQ=255)          gAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAgg                                                                                                                         <  2:468701/135‑1 (MQ=255)               cGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAAccc                                                                                                                            >  2:663622/1‑127 (MQ=255)               cGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAAccc                                                                                                                            <  1:663622/127‑1 (MQ=255)               cGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTg                                                                                                               >  2:1527488/1‑140 (MQ=255)               cGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTg                                                                                                               >  1:107576/1‑140 (MQ=255)                 tAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCt                                                                                                             >  1:1176541/1‑140 (MQ=255)                    cGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCAc                                                                                                          >  2:1592859/1‑140 (MQ=255)                       ggTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACcgc                                                                                                       >  2:1345175/1‑140 (MQ=255)                         tttCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCa                                                                                                           <  1:400548/134‑1 (MQ=255)                         tttCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCa                                                                                                           >  2:400548/1‑134 (MQ=255)                         tttCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGt                                                                                                     <  2:90767/140‑1 (MQ=255)                           tCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTg                                                                                                   >  2:1241447/1‑140 (MQ=255)                             tttACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCg                                                                                                 <  2:552538/140‑1 (MQ=255)                              ttACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCgg                                                                                                <  2:1176541/140‑1 (MQ=255)                                     ttttCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAg                                                                                         >  1:1077467/1‑140 (MQ=255)                                      tttCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCg                                                                                                 >  2:5238/1‑131 (MQ=255)                                      tttCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCg                                                                                                 <  1:5238/131‑1 (MQ=255)                                       ttCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGcc                                                                                       >  1:998835/1‑140 (MQ=255)                                         cAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGc                                                                                     <  2:998835/140‑1 (MQ=255)                                         cAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGc                                                                                     <  1:1345175/140‑1 (MQ=255)                                          aaCTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCt                                                                                    <  1:1592859/140‑1 (MQ=255)                                           aCTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTg                                                                                   >  2:200399/1‑140 (MQ=255)                                            cTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGa                                                                                  >  1:648740/1‑140 (MQ=255)                                               gATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCa                                                                               >  2:1455696/1‑140 (MQ=255)                                               gATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCa                                                                               >  1:908653/1‑140 (MQ=255)                                                 tGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATc                                                                             >  1:1628799/1‑140 (MQ=255)                                                    gTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGcc                                                                          <  2:1077467/140‑1 (MQ=255)                                                    gTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGcc                                                                          <  1:1609959/140‑1 (MQ=255)                                                    gTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGcc                                                                          <  1:870306/140‑1 (MQ=255)                                                           tttATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCt                                                                   >  2:386256/1‑140 (MQ=255)                                                             tATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCt                                                                       >  1:982648/1‑134 (MQ=255)                                                             tATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCt                                                                       <  2:982648/134‑1 (MQ=255)                                                              aTCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCg                                                                >  2:752537/1‑140 (MQ=255)                                                                       cTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGTGGGATGATGt                                                       <  1:493150/140‑1 (MQ=255)                                                                         tAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCg                                                     <  2:519453/140‑1 (MQ=255)                                                                         tAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCg                                                     <  2:1454915/140‑1 (MQ=255)                                                                         tAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCg                                                     <  2:931472/140‑1 (MQ=255)                                                                         tAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCg                                                     <  1:1455696/140‑1 (MQ=255)                                                                            aCGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTg                                                  <  1:1241447/140‑1 (MQ=255)                                                                              gCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCGCCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGcc                                                <  2:1628799/140‑1 (MQ=255)                                                                                caccacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGt                                              >  1:1638304/1‑140 (MQ=255)                                                                                caccacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGt                                              >  1:1083709/1‑140 (MQ=255)                                                                                 accacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCg                                                     <  1:1201128/132‑1 (MQ=255)                                                                                 accacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCg                                                     <  1:737857/132‑1 (MQ=255)                                                                                 accacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCg                                                     >  1:753019/1‑132 (MQ=255)                                                                                 accacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCg                                                     >  2:1201128/1‑132 (MQ=255)                                                                                 accacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCg                                                     >  2:737857/1‑132 (MQ=255)                                                                                 accacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCg                                                     <  2:753019/132‑1 (MQ=255)                                                                                 accacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGc                                                    <  1:1559966/133‑1 (MQ=255)                                                                                 accacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGc                                                    >  2:1559966/1‑133 (MQ=255)                                                                                 accacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTg                                                  <  2:670959/135‑1 (MQ=255)                                                                                 accacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTg                                                  >  1:670959/1‑135 (MQ=255)                                                                                 accacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGta                                             >  1:1130083/1‑140 (MQ=255)                                                                                 accacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGta                                             >  2:1585767/1‑140 (MQ=255)                                                                                 accacATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGta                                             >  2:922322/1‑140 (MQ=255)                                                                                     caTCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAAc                                         <  1:135249/140‑1 (MQ=255)                                                                                     caTCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAAc                                         <  2:1346333/140‑1 (MQ=255)                                                                                     caTCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAAc                                         <  2:1083709/140‑1 (MQ=255)                                                                                     caTCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAAc                                         <  1:200399/140‑1 (MQ=255)                                                                                       tCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCg                                                     >  2:430060/1‑126 (MQ=255)                                                                                       tCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCg                                                     <  1:430060/126‑1 (MQ=255)                                                                                       tCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGc                                       <  2:1480010/140‑1 (MQ=255)                                                                                       tCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGc                                       <  1:1585767/140‑1 (MQ=255)                                                                                       tCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGc                                       <  2:107576/140‑1 (MQ=255)                                                                                       tCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGc                                       <  1:752537/140‑1 (MQ=255)                                                                                       tCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGc                                       <  2:311593/140‑1 (MQ=255)                                                                                        ccTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCa                                      <  1:329471/140‑1 (MQ=255)                                                                                         cTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCAt                                     <  2:908653/140‑1 (MQ=255)                                                                                           gATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCg                                   <  2:559723/140‑1 (MQ=255)                                                                                           gATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCg                                   <  1:386256/140‑1 (MQ=255)                                                                                            aTAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGc                                       >  2:32137/1‑135 (MQ=255)                                                                                            aTAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGc                                       <  1:32137/135‑1 (MQ=255)                                                                                            aTAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGt                                  >  2:1597039/1‑140 (MQ=255)                                                                                            aTAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGt                                  >  2:1516550/1‑140 (MQ=255)                                                                                                cTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTa                                 <  1:119217/137‑1 (MQ=255)                                                                                                cTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTa                                 >  2:119217/1‑137 (MQ=255)                                                                                                cTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAg                              >  2:172690/1‑140 (MQ=255)                                                                                                 tGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGt                             >  2:322040/1‑140 (MQ=255)                                                                                                 tGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGt                             >  1:1090686/1‑140 (MQ=255)                                                                                                 tGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGt                             <  1:1597039/140‑1 (MQ=255)                                                                                                 tGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGt                             <  1:1516550/140‑1 (MQ=255)                                                                                                  gCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTg                            >  2:1158905/1‑140 (MQ=255)                                                                                                   ccGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAAc                                         >  2:263944/1‑126 (MQ=255)                                                                                                   ccGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAAc                                         <  1:263944/126‑1 (MQ=255)                                                                                                   ccGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAAc                                         >  2:138114/1‑126 (MQ=255)                                                                                                   ccGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAAc                                         <  1:138114/126‑1 (MQ=255)                                                                                                   ccGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGa                           >  2:868789/1‑140 (MQ=255)                                                                                                   ccGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATAATGTCGCTGCCGTATAACGCATCGTACAGTGa                           <  2:1356046/140‑1 (MQ=255)                                                                                                              cGGTAGAGATTCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCagcgag                >  2:656306/1‑140 (MQ=255)                                                                                                                        tCATCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTcgccgc      >  2:632086/1‑140 (MQ=255)                                                                                                                           tCGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGtt   <  1:1527488/140‑1 (MQ=255)                                                                                                                            cGAGGAAACGCCAAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTc  >  2:511707/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                ACTGTGCTGGAGTACGTAACGTGGTTTCTTTACCATTTTTCAACTGGATGCGTAATTGTTTATCAACCACCTTAAACGCCACCACATCCTGATAGCTGCCGTTTTCCAGCGGTAGAGATTCATCGAGGAAACGCCGAACCCAGGCGATAACCTGCTCACCGCGTTGCGGATCGTAGCCGCTGACCATCGCCCCTTCCTGCGGGATGATGTCGCTGCCGTATAACGCATCGTACAGTGAGCCCCAGCGAGCGTTCGCCGCGTTC  >  NC_000913/3123160‑3123422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑