Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,292,429	G→A	R363R (CGG→CGA)	yraK →	putative fimbrial adhesin YraK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,292,429	0	G	A	100.0%	274.8 / NA	78	R363R (CGG→CGA)	yraK	putative fimbrial adhesin YraK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/38);  total (40/38)

GAACGGTATCCTTCCCGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGGCCAGCGCCGCCGCTTTTTTCAGCGGC  >  NC_000913/3292297‑3292565                                                                                                                                     |                                                                                                                                         gAACGGTATCCTTCCCGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAaaga                                                                                                                                   >  1:247963/1‑140 (MQ=255)       tATCCTTCCCGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAaaga                                                                                                                                   >  1:170568/1‑134 (MQ=255)       tATCCTTCCCGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGt                                                                                                                             <  2:638437/140‑1 (MQ=255)       tATCCTTCCCGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGt                                                                                                                             >  1:1178513/1‑140 (MQ=255)       tATCCTTCCCGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGt                                                                                                                             >  1:154903/1‑140 (MQ=255)       tATCCTTCCCGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGt                                                                                                                             >  2:382779/1‑140 (MQ=255)       tATCCTTCCCGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGCCATCGTGCGATAAaaga                                                                                                                                   <  2:170568/134‑1 (MQ=255)        aTCCTTCCCGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGtt                                                                                                                            <  1:570797/140‑1 (MQ=255)             tCCCGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGtt                                                                                                                       <  2:1207039/140‑1 (MQ=255)             tCCCGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGtt                                                                                                                       <  2:559612/140‑1 (MQ=255)              cccGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGttt                                                                                                                      >  1:325034/1‑140 (MQ=255)              cccGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCCGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGttt                                                                                                                      >  1:1418323/1‑140 (MQ=255)                 gTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGtt                                                                                                                            >  1:1344132/1‑131 (MQ=255)                 gTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGtt                                                                                                                            <  2:1344132/131‑1 (MQ=255)                 gTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGa                                                                                                                   >  1:573929/1‑140 (MQ=255)                 gTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGa                                                                                                                   >  1:748780/1‑140 (MQ=255)                  ttGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGAt                                                                                                                  <  1:1326013/140‑1 (MQ=255)                    gATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTa                                                                                                                >  1:1095668/1‑140 (MQ=255)                     aTCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTaa                                                                                                               >  2:998919/1‑140 (MQ=255)                     aTCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTaa                                                                                                               >  1:172314/1‑140 (MQ=255)                           ccGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTaacgcaac                                                                                                         >  1:157647/1‑140 (MQ=255)                               gCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAGCGCAACGCtt                                                                                                     <  2:1095668/140‑1 (MQ=255)                                 aTGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCg                                                                                                   <  2:1255811/140‑1 (MQ=255)                                 aTGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCg                                                                                                   <  1:998919/140‑1 (MQ=255)                                 aTGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGAT‑AAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCgg                                                                                                  <  2:1261940/140‑1 (MQ=255)                                  tGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCgg                                                                                                  <  1:222884/140‑1 (MQ=255)                                  tGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCgg                                                                                                  <  2:172314/140‑1 (MQ=255)                                   ggCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGc                                                                                                 >  2:154292/1‑140 (MQ=255)                                     cACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCAc                                                                                               >  2:412738/1‑140 (MQ=255)                                           cAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTAc                                                                                         <  1:1632737/140‑1 (MQ=255)                                               atGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCct                                                                                     >  1:877375/1‑140 (MQ=255)                                                  cgcgcCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCgg                                                                                                  >  1:247267/1‑124 (MQ=255)                                                  cgcgcCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCgg                                                                                                  <  2:247267/124‑1 (MQ=255)                                                    cgcCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGAGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCtgctgc                                                                                >  2:538102/1‑140 (MQ=255)                                                     gcCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTgctgct                                                                               <  2:877375/140‑1 (MQ=255)                                                      ccTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTc                                                                              >  1:1456041/1‑140 (MQ=255)                                                      ccTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTc                                                                              >  1:1224057/1‑140 (MQ=255)                                                      ccTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTc                                                                              >  2:1260786/1‑140 (MQ=255)                                                         ggCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAg                                                                           <  1:412738/140‑1 (MQ=255)                                                           ccAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCACCGCTTCGGCACTGTTACCCCTGCTGCTCCAgcg                                                                         <  1:215424/140‑1 (MQ=255)                                                           ccAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTGCCCCTGCTGCTCCAgcg                                                                         <  2:247963/140‑1 (MQ=255)                                                           ccAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAgcg                                                                         <  2:507988/140‑1 (MQ=255)                                                           ccAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAgcg                                                                         <  1:1260786/140‑1 (MQ=255)                                                           ccAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAgcg                                                                         <  2:1224057/140‑1 (MQ=255)                                                           ccAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAgcg                                                                         <  1:468165/140‑1 (MQ=255)                                                           ccAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAgcg                                                                         <  2:748780/140‑1 (MQ=255)                                                           ccAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAgcg                                                                         <  1:382779/140‑1 (MQ=255)                                                           ccAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAgcg                                                                         <  2:1456041/140‑1 (MQ=255)                                                             aGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCa                                                                       <  2:949265/140‑1 (MQ=255)                                                             aGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCa                                                                       <  2:154903/140‑1 (MQ=255)                                                                   tCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTta                                                                 >  1:485876/1‑140 (MQ=255)                                                                     tGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTtata                                                               <  1:192158/140‑1 (MQ=255)                                                                     tGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTtata                                                               >  1:915732/1‑140 (MQ=255)                                                                      ggTCGGTGGTGAGCTGGAAACAGGAAAATTTAAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATAc                                                              >  1:880200/1‑140 (MQ=255)                                                                       gTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACa                                                             >  2:501240/1‑140 (MQ=255)                                                                        tCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAgcg                                                                         >  1:1473250/1‑127 (MQ=255)                                                                        tCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAgcg                                                                         <  2:1615096/127‑1 (MQ=255)                                                                        tCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAgcg                                                                         <  2:1473250/127‑1 (MQ=255)                                                                        tCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAgcg                                                                         >  1:1615096/1‑127 (MQ=255)                                                                        tCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAg                                                            >  2:840645/1‑140 (MQ=255)                                                                         cGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAgc                                                           >  1:212973/1‑140 (MQ=255)                                                                            tggtgAGCTGGAAACAGGAAAATTTAAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCa                                                        <  2:880200/140‑1 (MQ=255)                                                                                 accTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCAttttt                                                   >  1:1474033/3‑140 (MQ=255)                                                                                      gAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCa                                              >  2:1125998/1‑140 (MQ=255)                                                                                        taCAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACg                                            >  2:1065811/2‑140 (MQ=255)                                                                                        aaCAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACg                                            <  1:154292/140‑1 (MQ=255)                                                                                                                 cccaTTACCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGGCCAGcgccg                   <  2:212973/139‑1 (MQ=255)                                                                                                                       aCCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGGCCAGCGCCGCCGCtt             >  2:47521/1‑140 (MQ=255)                                                                                                                       aCCGTCATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGGCCAGCGCCGCCGCtt             >  2:1081912/1‑140 (MQ=255)                                                                                                                            cATCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGGCCAGCGCCGCCGCTTTTTTc        <  2:915732/140‑1 (MQ=255)                                                                                                                             aTCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGGCCAGCGCCGCCGCTTTTTTCa       >  2:1409546/1‑140 (MQ=255)                                                                                                                             aTCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGGCCAGCGCCGCCGCTTTTTTCa       >  1:493995/1‑140 (MQ=255)                                                                                                                             aTCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGGCCAGCGCCGCCGCTTTTTTCa       >  1:936476/1‑140 (MQ=255)                                                                                                                             aTCGTGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGGCCAGCGCCGCCGCTTTTTTCa       >  1:1053385/1‑140 (MQ=255)                                                                                                                                 tGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGGCCAgcgc                     >  1:1132064/1‑122 (MQ=255)                                                                                                                                 tGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGGCCAgcgc                     <  2:1132064/122‑1 (MQ=255)                                                                                                                                 tGCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGGCCAGCGCCGCCGCTTTTTTCAGCgg   <  2:1053385/140‑1 (MQ=255)                                                                                                                                  gCGATAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGGCCAGCGCCGCCGCTTTTTTCAGCGGc  <  1:1065811/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         GAACGGTATCCTTCCCGTTGATCCTTCCGGGCATGGCACCGTCAATATGCGCGCCTGGCCAGTTAATCTGGTCGGTGGTGAGCTGGAAACAGGAAAATTTCAGGGCACAGCCACCATTACCGTCATCGTGCGGTAAAAGAAGACGTTGCGTTTTGATTAACGCAACGCTTCGGCACTGTTACCCCTGCTGCTCCAGCGCATACTTATACAGCGCATTTTTCTTCACGCCGTGAATTTCTGCGGCCAGCGCCGCCGCTTTTTTCAGCGGC  >  NC_000913/3292297‑3292565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑