Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,744,307	A→G	intergenic (+97/‑21)	yiaL → / → yiaM	DUF386 domain‑containing protein YiaL/2,3‑diketo‑L‑gulonate:Na(+) symporter ‑ membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,744,307	0	A	G	100.0%	320.1 / NA	89	intergenic (+97/‑21)	yiaL/yiaM	DUF386 domain‑containing protein YiaL/2,3‑diketo‑L‑gulonate:Na(+) symporter ‑ membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (45/44);  total (45/44)

TGTGGTTAAAGTCGCATTAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGAT  >  NC_000913/3744177‑3744441                                                                                                                                   |                                                                                                                                       tgtgGTTAAAGTCGCATTAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTc                                                                                                                               <  1:304770/140‑1 (MQ=255)  gtgGTTAAAGTCGCATTAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCa                                                                                                                              <  2:992815/140‑1 (MQ=255)  gtgGTTAAAGTCGCATTAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCa                                                                                                                              <  2:883480/140‑1 (MQ=255)  gtgGTTAAAGTCGCATTAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCa                                                                                                                              <  1:1215936/140‑1 (MQ=255)    ggTTAAAGTCGCATTAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAg                                                                                                                            >  2:396581/1‑140 (MQ=255)       tAAAGTCGCATTAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGaa                                                                                                                         <  1:396581/140‑1 (MQ=255)        aaaGTCGCATTAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAAt                                                                                                                        >  1:1531380/1‑140 (MQ=255)        aaaGTCGCATTAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAAt                                                                                                                        >  1:1291394/1‑140 (MQ=255)               cATTAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATg                                                                                                                 >  1:378526/1‑140 (MQ=255)                    aCGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGaaaaa                                                                                                            >  1:1133428/1‑140 (MQ=255)                     cGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAATTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGaaaaaa                                                                                                           <  2:14617/140‑1 (MQ=255)                          cTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACt                                                                                                      <  2:356513/140‑1 (MQ=255)                          cTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACt                                                                                                      >  1:592790/1‑140 (MQ=255)                          cTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACt                                                                                                      <  2:818073/140‑1 (MQ=255)                          cTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACt                                                                                                      >  1:1553369/1‑140 (MQ=255)                             aaTTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGa                                                                                                   >  2:953537/1‑140 (MQ=255)                             aaTTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGa                                                                                                   <  2:577243/140‑1 (MQ=255)                               ttAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAg                                                                                                 <  2:592790/140‑1 (MQ=255)                               ttAATCGAATAATCGTCCACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGCCAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAg                                                                                                 <  2:1478649/140‑1 (MQ=255)                                      aataatCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACt                                                                                          <  2:1544749/140‑1 (MQ=255)                                      aataatCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCCAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACt                                                                                          <  2:1146967/140‑1 (MQ=255)                                            cGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGAt                                                                                    <  2:1229893/140‑1 (MQ=255)                                                tACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAAt                                                                                >  2:285916/1‑140 (MQ=255)                                                      aGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGcc                                                                          <  1:106689/140‑1 (MQ=255)                                                       gAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCg                                                                         >  2:667544/1‑140 (MQ=255)                                                        aaCGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGt                                                                        >  1:175949/1‑140 (MQ=255)                                                        aaCGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGt                                                                        >  1:95311/1‑140 (MQ=255)                                                           gCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACt                                                                     <  2:988779/140‑1 (MQ=255)                                                           gCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACt                                                                     <  2:175949/140‑1 (MQ=255)                                                            ccTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACtt                                                                    >  2:823876/1‑140 (MQ=255)                                                              tGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTc                                                                  <  1:1163700/140‑1 (MQ=255)                                                              tGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTc                                                                  <  2:378526/140‑1 (MQ=255)                                                              tGGGTAATGTTATTGCTCTAACTATACTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTc                                                                  <  1:603890/140‑1 (MQ=255)                                                                   aaTGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCtgta                                                             <  1:667544/140‑1 (MQ=255)                                                                    aTGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCtgtat                                                            <  1:832848/140‑1 (MQ=255)                                                                     tGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACt                                                                     <  2:887345/130‑1 (MQ=255)                                                                     tGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACt                                                                     >  1:887345/1‑130 (MQ=255)                                                                      gTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATGGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCtgtattg                                                          <  2:694888/140‑1 (MQ=255)                                                                      gTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCtgtattg                                                          <  1:285916/140‑1 (MQ=255)                                                                          ttGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTa                                                                       <  1:1080020/123‑1 (MQ=255)                                                                          ttGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTa                                                                       >  2:1080020/1‑123 (MQ=255)                                                                          ttGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTgtattgtatt                                                      >  1:335744/1‑140 (MQ=255)                                                                           tGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTAttt                                                     >  2:721643/1‑140 (MQ=255)                                                                             ctctAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTtat                                                   <  1:128689/140‑1 (MQ=255)                                                                               ctAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATaa                                                 >  1:1621548/1‑140 (MQ=255)                                                                               ctAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATaa                                                 >  2:706103/1‑140 (MQ=255)                                                                                tAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATaaa                                                >  2:1412934/1‑140 (MQ=255)                                                                                tAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATaaa                                                >  1:194416/1‑140 (MQ=255)                                                                                tAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATaaa                                                >  2:1065070/1‑140 (MQ=255)                                                                                 aaCTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCtgtattg                                                          <  1:1095993/129‑1 (MQ=255)                                                                                 aaCTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAAc                                               >  1:961895/1‑140 (MQ=255)                                                                                 aaCTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAAc                                               >  1:165074/1‑140 (MQ=255)                                                                                 aaCTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTAATTTCCtgtattg                                                          >  2:1095993/1‑129 (MQ=255)                                                                                  aCTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACa                                              >  2:97361/1‑140 (MQ=255)                                                                                  aCTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACa                                              >  2:121196/1‑140 (MQ=255)                                                                                    tataTTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACatt                                            >  2:221346/1‑140 (MQ=255)                                                                                       atTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACa                                              <  2:997511/135‑1 (MQ=255)                                                                                       atTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACa                                              >  1:997511/1‑135 (MQ=255)                                                                                        ttAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCtgtattg                                                          <  1:282008/122‑1 (MQ=255)                                                                                        ttAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCtgtattg                                                          >  2:282008/1‑122 (MQ=255)                                                                                         taataaTTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTAtttt                                       <  1:1265899/140‑1 (MQ=255)                                                                                            taatTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTAttt                                        <  1:1632296/136‑1 (MQ=255)                                                                                            taatTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTAttt                                        >  2:1632296/1‑136 (MQ=255)                                                                                            taatTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAg                                    >  2:1259096/1‑140 (MQ=255)                                                                                            taatTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAg                                    >  1:1476071/1‑140 (MQ=255)                                                                                              atTCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGat                                  >  2:325109/1‑140 (MQ=255)                                                                                               ttCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGata                                 <  1:1065070/140‑1 (MQ=255)                                                                                               ttCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGata                                 <  1:1133964/140‑1 (MQ=255)                                                                                               ttCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGata                                 <  1:1112781/140‑1 (MQ=255)                                                                                                tCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGatat                                >  2:1621659/1‑140 (MQ=255)                                                                                                tCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGatat                                >  2:564082/1‑140 (MQ=255)                                                                                                tCAGGTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGatat                                >  2:145061/1‑140 (MQ=255)                                                                                                    gTTGCTGCACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGtt                            >  1:533256/1‑140 (MQ=255)                                                                                                      tgctgcACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGtttt                          >  2:537231/1‑140 (MQ=255)                                                                                                        ctgcACATGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCa                        >  1:258808/1‑140 (MQ=255)                                                                                                            acaTGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACa                    <  1:145061/140‑1 (MQ=255)                                                                                                            acaTGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACa                    <  2:1291394/140‑1 (MQ=255)                                                                                                              aTGCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAg                  >  1:1211972/1‑140 (MQ=255)                                                                                                                gCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGata                                 >  2:1082043/1‑123 (MQ=255)                                                                                                                gCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGata                                 <  1:1082043/123‑1 (MQ=255)                                                                                                                gCGACTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCa                >  1:596864/1‑140 (MQ=255)                                                                                                                   aCTTGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCAttt             >  2:125654/1‑140 (MQ=255)                                                                                                                     ttGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTa           >  1:1475918/1‑140 (MQ=255)                                                                                                                      tGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTAt          <  1:564082/140‑1 (MQ=255)                                                                                                                      tGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTAt          <  1:1621659/140‑1 (MQ=255)                                                                                                                      tGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTAt          <  1:125654/140‑1 (MQ=255)                                                                                                                      tGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTAt          <  1:121196/140‑1 (MQ=255)                                                                                                                      tGAATTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTAt          <  2:1475918/140‑1 (MQ=255)                                                                                                                         aTTATGACAGGCATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTg       <  2:165074/140‑1 (MQ=255)                                                                                                                              gacaggcaTAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTtgat  <  1:823876/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                       TGTGGTTAAAGTCGCATTAACGGCGCTGAATTAATCGAATAATCGTCTACGCCAGAACGCCTGGGTAATGTTATTGCTCTAACTATATTAATAATTCAGGTTGCTGCACATGCGACTTGAATTATGACAGACATAGCCTCAAGGAATAGCTATGAAAAAAATACTCGAAGCAATACTGGCGATTAATCTCGCCGTACTTTCCTGTATTGTATTTATAAACATTATTTTAAGATATGGTTTTCAGACAAGCATTTTATCTGTTGAT  >  NC_000913/3744177‑3744441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑