Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,858,049	T→C	D119G (GAC→GGC)	yidJ ←	putative sulfatase/phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,858,049	0	T	C	100.0%	347.8 / NA	97	D119G (GAC→GGC)	yidJ	putative sulfatase/phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (54/43);  total (54/43)

TGCGCCCAGGTGAAGGTTTCGTCGATATGGTTCGCCTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGTCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTT  >  NC_000913/3857914‑3858185                                                                                                                                        |                                                                                                                                         tGCGCCCAGGTGAAGGTTTCGTCGATATGGTTCGCCTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCa                                                                                                                                      <  1:904074/140‑1 (MQ=255)  gcgcCCAGGTGAAGGTTTCGTCGATATGGTTCGCCTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCAc                                                                                                                                     >  1:1146284/1‑140 (MQ=255)   cgcCCAGGTGAAGGTTTCGTCGATATGGTTCGCCTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACt                                                                                                                                    <  2:1009117/140‑1 (MQ=255)     cccAGGTGAAGGTTTCGTCGATATGGTTCGCCTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACt                                                                                                                                    <  2:1643504/138‑1 (MQ=255)     cccAGGTGAAGGTTTCGTCGATATGGTTCGCCTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACt                                                                                                                                    >  1:1643504/1‑138 (MQ=255)     cccAGGTGAAGGTTTCGTCGATATGGTTCGCCTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTcc                                                                                                                                  >  2:1003223/1‑140 (MQ=255)     cccAGGTGAAGGTTTCGTCGATATGGTTCGCCTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTcc                                                                                                                                  >  1:416284/1‑140 (MQ=255)     cccAGGTGAAGGTTTCGTCGATATGGTTCGCCTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTcc                                                                                                                                  >  1:1369865/1‑140 (MQ=255)             aaGGTTTCGTCGATATGGTTCGCCTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGaca                                                                                                                          >  1:1059225/1‑140 (MQ=255)                  ttCGTCGATATGGTTCGCCTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGc                                                                                                                     <  1:1543664/140‑1 (MQ=255)                  ttCGTCGATATGGTTCGCCTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGACGGACACTCGc                                                                                                                     <  2:448547/140‑1 (MQ=255)                    cgtcgATATGGTTCGCCTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCa                                                                                                                   >  1:38338/1‑140 (MQ=255)                    cgtcgATATGGTTCGCCTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCa                                                                                                                   >  2:808001/1‑140 (MQ=255)                    cgtcgATATGGTTCGCCTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCa                                                                                                                   <  1:808001/140‑1 (MQ=255)                     gtcgATATGGTTCGCCTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAg                                                                                                                  <  2:1161295/140‑1 (MQ=255)                     gtcgATATGGTTCGCCTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAg                                                                                                                  >  1:1161295/1‑140 (MQ=255)                     gtcgATATGGTTCGCCTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAg                                                                                                                  >  2:509334/1‑140 (MQ=255)                            tGGTTCGCCTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGaa                                                                                                           <  1:1105544/140‑1 (MQ=255)                            tGGTTCGCCTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGaa                                                                                                           <  2:1625345/140‑1 (MQ=255)                                   ccTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCa                                                                                                    <  1:1098033/140‑1 (MQ=255)                                    cTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGc                                                                                                                     <  1:1079312/122‑1 (MQ=255)                                    cTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGc                                                                                                                     >  2:1079312/1‑122 (MQ=255)                                    cTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCAt                                                                                                   <  2:1059225/140‑1 (MQ=255)                                    cTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCAt                                                                                                   >  1:1100262/1‑140 (MQ=255)                                      gTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGa                                                                                                 >  2:536299/1‑140 (MQ=255)                                       tAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGAc                                                                                                >  2:812159/1‑140 (MQ=255)                                              tCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCgag                                                                                         <  2:884714/140‑1 (MQ=255)                                                 aCGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATg                                                                                      >  1:1236472/1‑140 (MQ=255)                                                       tttAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCAttt                                                                                <  1:536299/140‑1 (MQ=255)                                                         tAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGc                                                                              <  1:980070/140‑1 (MQ=255)                                                          aGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGcc                                                                             <  2:1619447/140‑1 (MQ=255)                                                            gCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGa                                                                           >  2:1580054/1‑140 (MQ=255)                                                               aTTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGt                                                                        >  1:692212/1‑140 (MQ=255)                                                                 tccgcCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTaa                                                                      <  2:1537164/138‑1 (MQ=255)                                                                 tGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTaa                                                                      >  2:1178153/1‑140 (MQ=255)                                                                 tGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTaa                                                                      >  2:278240/1‑140 (MQ=255)                                                                 tGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTaa                                                                      >  1:224867/1‑140 (MQ=255)                                                                 tGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTaa                                                                      >  2:841378/1‑140 (MQ=255)                                                                 tGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTaa                                                                      >  1:1368899/1‑140 (MQ=255)                                                                 tGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTaa                                                                      >  1:1261632/1‑140 (MQ=255)                                                                 tGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTa                                                                       <  1:627261/139‑1 (MQ=255)                                                                 tGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTa                                                                       >  2:627261/1‑139 (MQ=255)                                                                  gcgcCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAAc                                                                     <  1:509334/140‑1 (MQ=255)                                                                    gcCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAg                                                                   <  1:1312108/140‑1 (MQ=255)                                                                        caGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGgtgt                                                               <  2:1261632/140‑1 (MQ=255)                                                                             tAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTa                                                                       >  2:930228/1‑127 (MQ=255)                                                                             tAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTa                                                                       <  1:930228/127‑1 (MQ=255)                                                                                tctcTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCg                                                       >  1:1436350/1‑140 (MQ=255)                                                                                tctcTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCg                                                       >  2:479876/1‑140 (MQ=255)                                                                                 ctctTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCgg                                                      <  1:400571/140‑1 (MQ=255)                                                                                  tctTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGc                                                     <  1:1178153/140‑1 (MQ=255)                                                                                  tctTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGc                                                     <  1:58398/140‑1 (MQ=255)                                                                                  tctTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGc                                                     <  2:610375/140‑1 (MQ=255)                                                                                  tctTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGc                                                     <  1:278240/140‑1 (MQ=255)                                                                                  tctTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGc                                                     <  2:1359408/140‑1 (MQ=255)                                                                                     ttttCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATc                                                  >  2:812609/1‑140 (MQ=255)                                                                                     ttttCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATc                                                  >  1:1507246/1‑140 (MQ=255)                                                                                         ccGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTa                                              >  2:855513/1‑140 (MQ=255)                                                                                            tCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGc                                                     >  1:1174577/1‑130 (MQ=255)                                                                                            tCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGc                                                     <  2:1174577/130‑1 (MQ=255)                                                                                            tCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAg                                           >  1:891334/1‑140 (MQ=255)                                                                                              aGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTa                                         <  2:224867/140‑1 (MQ=255)                                                                                              aGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTa                                         >  1:360779/1‑140 (MQ=255)                                                                                              aGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTa                                         >  1:445115/1‑140 (MQ=255)                                                                                               gTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAg                                        <  2:891334/140‑1 (MQ=255)                                                                                               gTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAg                                        <  1:812609/140‑1 (MQ=255)                                                                                                 tCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAgcg                                      <  2:445115/140‑1 (MQ=255)                                                                                                 tCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAgcg                                      <  2:360779/140‑1 (MQ=255)                                                                                                    cTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGccc                                   >  2:854625/1‑140 (MQ=255)                                                                                                          tAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGt                             >  1:51136/1‑140 (MQ=255)                                                                                                          tAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGt                             >  1:388653/1‑140 (MQ=255)                                                                                                          tAGTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGt                             >  1:366307/1‑140 (MQ=255)                                                                                                            gTTCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTgg                           >  1:910123/1‑140 (MQ=255)                                                                                                             ttCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAgcg                                      >  2:723115/1‑128 (MQ=255)                                                                                                             ttCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAgcg                                      <  1:723115/128‑1 (MQ=255)                                                                                                             ttCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGcc                                    <  2:440708/130‑1 (MQ=255)                                                                                                             ttCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGcc                                    >  1:440708/1‑130 (MQ=255)                                                                                                             ttCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGga                          >  2:347115/1‑140 (MQ=255)                                                                                                             ttCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGga                          >  1:968432/1‑140 (MQ=255)                                                                                                             ttCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGga                          >  2:457765/1‑140 (MQ=255)                                                                                                             ttCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGga                          >  2:131552/1‑140 (MQ=255)                                                                                                             ttCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGga                          >  2:1536045/1‑140 (MQ=255)                                                                                                             ttCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGga                          >  2:556160/1‑140 (MQ=255)                                                                                                             ttCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGga                          >  2:74113/1‑140 (MQ=255)                                                                                                             ttCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCACCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGga                          >  1:1173054/1‑140 (MQ=255)                                                                                                              tCGCCCCATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGgag                         <  1:347115/140‑1 (MQ=255)                                                                                                                  cccATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGGAGATGt                     >  2:1435214/1‑140 (MQ=255)                                                                                                                  cccATCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTGAAAGTAGCGCCCCATAGTGGAGATGt                     >  1:1161140/1‑140 (MQ=255)                                                                                                                     aTCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCa                                 <  2:315842/125‑1 (MQ=255)                                                                                                                     aTCGAACCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCa                                 >  1:315842/1‑125 (MQ=255)                                                                                                                         aaCCAGTAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGGAGATGTTTTTGcc              <  2:1436350/140‑1 (MQ=255)                                                                                                                               tAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGGAGATGTTTTTGCCTGgcgc        <  1:457765/140‑1 (MQ=255)                                                                                                                               tAATCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGGAGATGTTTTTGCCTGgcgc        <  1:855513/140‑1 (MQ=255)                                                                                                                                 aTCAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGGAGATGTTTTTGCCTGGCGCGa      <  2:1161140/140‑1 (MQ=255)                                                                                                                                   cAGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGGAGATGTTTTTGCCTGGCGCGACg    <  1:1580054/140‑1 (MQ=255)                                                                                                                                    aGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGGAGATGTTTTTGCCTGGCGCGACgt   >  1:1486517/1‑140 (MQ=255)                                                                                                                                    aGCGCCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGGAGATGTTTTTGCCTGGCGCGACgt   >  2:1397759/1‑140 (MQ=255)                                                                                                                                     gcgcCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGGAGATGTTTTTGCCTGGCGCGACgtt  >  2:1090127/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                         TGCGCCCAGGTGAAGGTTTCGTCGATATGGTTCGCCTGTAAATCTTCGACGCTGTTTAGGCCATTGCGCCACAGGCTAATCTCTTTTTCCGTCAGTTCGCTAAGATAGTTCGCCCCATCGAACCAGTAATCAGCGTCCCACTCCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTT  >  NC_000913/3857914‑3858185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑