Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,980,280	A→G	T109A (ACG→GCG)	rffM →	UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,980,280	0	A	G	100.0%	255.6 / NA	72	T109A (ACG→GCG)	rffM	UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/38);  total (34/38)

CGCTGCCGAATTTAAATATGCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGACGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGTTATTTTAAACCCGAGCAGCGTCAGGCGCTGTT  >  NC_000913/3980144‑3980413                                                                                                                                         |                                                                                                                                      cGCTGCCGAATTTAAATATGCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGgcgc                                                                                                                                    <  2:1087624/140‑1 (MQ=255)  gCTGCCGAATTTAAATATGCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGcc                                                                                                                                   <  1:344832/140‑1 (MQ=255)  gCTGCCGAATTTAAATATGCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGcc                                                                                                                                   <  2:989282/140‑1 (MQ=255)  gCTGCCGAATTTAAATATGCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGcc                                                                                                                                   <  2:1121538/140‑1 (MQ=255)   cTGCCGAATTTAAATATGCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGgcgcag                                                                                                                                  >  2:850527/1‑138 (MQ=255)      ccGAATTTAAATATGCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTa                                                                                                                               >  2:154700/1‑140 (MQ=255)      ccGAATTTAAATATGCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTa                                                                                                                               >  2:888475/1‑140 (MQ=255)            ttAAATATGCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCtt                                                                                                                         <  1:1278405/140‑1 (MQ=255)                atatGCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTgg                                                                                                                     <  1:1659696/140‑1 (MQ=255)                atatGCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTgg                                                                                                                     <  1:154700/140‑1 (MQ=255)                 tatGCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTggg                                                                                                                    <  2:1639226/140‑1 (MQ=255)                    gCGGATGGCATCAGCGTTGTACGTTCGGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCgg                                                                                                                 <  1:614778/140‑1 (MQ=255)                    gCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCgg                                                                                                                 <  1:888475/140‑1 (MQ=255)                     cGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGt                                                                                                                >  1:450511/1‑140 (MQ=255)                     cGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGt                                                                                                                >  2:936308/1‑140 (MQ=255)                                 gCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTg                                                                                                    >  2:293383/1‑140 (MQ=255)                                   gTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCt                                                                                                  <  1:293383/140‑1 (MQ=255)                                     tGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTgg                                                                                                >  2:267822/1‑140 (MQ=255)                                      gTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGgc                                                                                               >  2:989311/1‑140 (MQ=255)                                       tACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGgcg                                                                                              >  2:1291890/1‑140 (MQ=255)                                            tCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAAc                                                                                         >  2:825039/1‑140 (MQ=255)                                            tCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAAc                                                                                         >  2:1585934/1‑140 (MQ=255)                                            tCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAAc                                                                                         >  1:1431580/1‑140 (MQ=255)                                            tCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAAc                                                                                         <  1:1140849/140‑1 (MQ=255)                                            tCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCAGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAAc                                                                                         <  1:850527/140‑1 (MQ=255)                                              aGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGTGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTg                                                                                       >  2:1123392/1‑140 (MQ=255)                                                 aCGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAg                                                                                    >  2:650451/1‑140 (MQ=255)                                                 aCGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAg                                                                                    >  1:504249/1‑140 (MQ=255)                                                 aCGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAg                                                                                    >  1:1286094/1‑140 (MQ=255)                                                   gTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCt                                                                                  >  1:269353/1‑140 (MQ=255)                                                    tAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTa                                                                                 <  1:1291890/140‑1 (MQ=255)                                                     aaaaaGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTaa                                                                                >  2:658254/1‑140 (MQ=255)                                                       aaaGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAAc                                                                              >  1:272185/1‑140 (MQ=255)                                                          gTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAAc                                                                                         >  2:979203/1‑126 (MQ=255)                                                          gTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAAc                                                                                         <  1:979203/126‑1 (MQ=255)                                                          gTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTaaa                                                                               >  2:654338/1‑136 (MQ=255)                                                          gTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTaaa                                                                               <  1:654338/136‑1 (MQ=255)                                                          gTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTgc                                                                           >  1:829237/1‑140 (MQ=255)                                                            aCCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTgcgc                                                                         <  2:450511/140‑1 (MQ=255)                                                            aCCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTgcgc                                                                         <  2:269353/140‑1 (MQ=255)                                                            aCCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTgcgc                                                                         <  2:937859/140‑1 (MQ=255)                                                             cccGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCa                                                                        >  1:514518/1‑140 (MQ=255)                                                              ccGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCaa                                                                       <  1:452627/140‑1 (MQ=255)                                                                gcaggcgcaggTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAACCTGCGCAAcc                                                                     <  2:715939/140‑1 (MQ=255)                                                                 caggcgcaggTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCa                                                                    <  1:15654/140‑1 (MQ=255)                                                                     cgcaggTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTgg                                                                <  1:825039/140‑1 (MQ=255)                                                                     cgcaggTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTgg                                                                <  1:1208871/140‑1 (MQ=255)                                                                     cgcaggTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTgg                                                                <  1:1585934/140‑1 (MQ=255)                                                                      gcaggTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGa                                                               <  1:658254/140‑1 (MQ=255)                                                                       caggTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGaa                                                              <  2:272185/140‑1 (MQ=255)                                                                        aggTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAAt                                                             <  1:1377072/140‑1 (MQ=255)                                                                        aggTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAAt                                                             <  2:1431580/140‑1 (MQ=255)                                                                            ttCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGa                                                         >  1:867660/1‑140 (MQ=255)                                                                                cgcgTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAatat                                                     <  2:742915/140‑1 (MQ=255)                                                                                        cGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCa                                             <  1:936308/140‑1 (MQ=255)                                                                                          gTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGt                                           <  2:198760/140‑1 (MQ=255)                                                                                                   tctGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGt                                  <  2:1286094/140‑1 (MQ=255)                                                                                                     tGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGTTa                                >  1:957338/1‑140 (MQ=255)                                                                                                        gAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGTTAttt                             <  2:1616538/140‑1 (MQ=255)                                                                                                            agCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGt                                  >  1:326560/1‑131 (MQ=255)                                                                                                            agCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGt                                  <  2:326560/131‑1 (MQ=255)                                                                                                            agCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGTTATTTTaaa                         >  1:1505075/1‑140 (MQ=255)                                                                                                            agCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGTTATTTTaaa                         >  1:1185054/1‑140 (MQ=255)                                                                                                            agCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGTTATTTTaaa                         >  1:854611/1‑140 (MQ=255)                                                                                                            agCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGTTATTTTaaa                         >  1:1247024/1‑140 (MQ=255)                                                                                                            agCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGTTATTTTaaa                         >  1:1436917/1‑140 (MQ=255)                                                                                                             gCTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGTTATTTTAAAc                        <  2:957338/140‑1 (MQ=255)                                                                                                              cTGATGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGTTATTTTAAAcc                       >  2:208486/1‑140 (MQ=255)                                                                                                                  tGGCGCGCGCAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGTTATTTTAAACCCGag                   <  2:1247024/140‑1 (MQ=255)                                                                                                                      gcgcgcAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGTTATTTTAAACCCGagcagc               >  1:43502/1‑140 (MQ=255)                                                                                                                      gcgcgcAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGTTATTTTAAACCCGagca                 <  2:180508/138‑1 (MQ=255)                                                                                                                      gcgcgcAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGTTATTTTAAACCCGagca                 >  1:180508/1‑138 (MQ=255)                                                                                                                        gcgcAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGTTATTTTAAACCCGAGCAGCGt             >  2:1322929/1‑140 (MQ=255)                                                                                                                           cAGGCAAAGAAGGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGTTATTTTAAACCCGAGCAGCGTCAg          >  2:959130/1‑140 (MQ=255)                                                                                                                                   gaagGGGCGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGTTATTTTAAACCCGAGCAGCGTCAGGCGCTGtt  <  2:514518/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      CGCTGCCGAATTTAAATATGCGGATGGCATCAGCGTTGTACGTTCAGTACGTAAAAAGTACCCGCAGGCGCAGGTTTCCCGCGTTGCCGGTGCCGATCTCTGGGAAGAGCTGATGGCGCGCGCAGGCAAAGAAGGGACGCCGGTATTTCTTGTGGGCGGTAAACCTGAAGTGCTGGCGCAAACTGAAGCTAAACTGCGCAACCAGTGGAATGTGAATATCGTTGGCAGTCAGGATGGTTATTTTAAACCCGAGCAGCGTCAGGCGCTGTT  >  NC_000913/3980144‑3980413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑