Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,023,449	T→C	G238G (GGT→GGC)	tatC →	twin arginine protein translocation system ‑ TatC protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,023,449	0	T	C	100.0%	189.8 / NA	54	G238G (GGT→GGC)	tatC	twin arginine protein translocation system ‑ TatC protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/30);  total (24/30)

CGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGAC  >  NC_000913/4023316‑4023579                                                                                                                                      |                                                                                                                                   cGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAggg                                                                                                                              >  2:68732/1‑140 (MQ=255) cGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAggg                                                                                                                              <  2:506835/140‑1 (MQ=255) cGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAggg                                                                                                                              <  2:1361273/140‑1 (MQ=255) cGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAggg                                                                                                                              <  1:726705/140‑1 (MQ=255)   tATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCg                                                                                                                            >  2:664257/1‑140 (MQ=255)        gCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATc                                                                                                                       <  1:141521/140‑1 (MQ=255)           ggttggtGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCggg                                                                                                                    <  1:1288583/140‑1 (MQ=255)               ggtGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAaga                                                                                                                >  2:979206/1‑140 (MQ=255)                      tCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGa                                                                                                             >  2:62827/1‑136 (MQ=255)                      tCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGa                                                                                                             <  1:62827/136‑1 (MQ=255)                          tGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAacgacg                                                                                                     <  1:979206/140‑1 (MQ=255)                          tGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAacgacg                                                                                                     <  2:1473484/140‑1 (MQ=255)                          tGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAacgacg                                                                                                     <  1:1553928/140‑1 (MQ=255)                              gggATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGa                                                                                                 <  1:1660114/140‑1 (MQ=255)                                 aTGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGc                                                                                              >  1:1202792/1‑140 (MQ=255)                                     tGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGaa                                                                                          <  2:687535/140‑1 (MQ=255)                                      gCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGaaa                                                                                         <  2:836917/140‑1 (MQ=255)                                       cTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAg                                                                                        >  2:818045/1‑140 (MQ=255)                                          aCGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAacgacg                                                                                                     <  1:819896/124‑1 (MQ=255)                                          aCGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAacgacg                                                                                                     >  2:819896/1‑124 (MQ=255)                                            gccgccGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGaaa                                                                                   >  2:1065236/1‑140 (MQ=255)                                                ccgGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAAc                                                                               >  2:517968/1‑140 (MQ=255)                                                  ggATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTg                                                                             <  2:1345161/140‑1 (MQ=255)                                                      gTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTgaaga                                                                         <  1:1065236/140‑1 (MQ=255)                                                          tctcGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATaa                                                                     >  1:1427805/1‑140 (MQ=255)                                                              gCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTc                                                                 >  1:951060/1‑140 (MQ=255)                                                                aaaCGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGTCGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCaa                                                               <  1:664257/140‑1 (MQ=255)                                                                             gATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAggg                                                  <  1:68732/140‑1 (MQ=255)                                                                             gATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAggg                                                  <  2:1427805/140‑1 (MQ=255)                                                                              aTCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCg                                                            >  2:1382117/1‑129 (MQ=255)                                                                              aTCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCg                                                            <  1:1382117/129‑1 (MQ=255)                                                                                cccGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCgg                                               <  2:728789/140‑1 (MQ=255)                                                                                   gATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTg                                            <  1:1318952/140‑1 (MQ=255)                                                                                   gATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTg                                            <  1:878441/140‑1 (MQ=255)                                                                                          tgtctgtTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATg                                     >  1:727673/1‑140 (MQ=255)                                                                                          tgtctgtTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATg                                     >  2:211230/1‑140 (MQ=255)                                                                                            tctgtTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCatat                                      <  1:838791/137‑1 (MQ=255)                                                                                            tctgtTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCatat                                      >  2:838791/1‑137 (MQ=255)                                                                                             ctgtTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAg                                  <  1:1134859/140‑1 (MQ=255)                                                                                               gtTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAggg                                                  <  1:946602/122‑1 (MQ=255)                                                                                               gtTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAggg                                                  >  2:946602/1‑122 (MQ=255)                                                                                               gtTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTa                                <  1:823474/140‑1 (MQ=255)                                                                                                    aaaTCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGa                           >  2:340310/1‑140 (MQ=255)                                                                                                      aTCGGTGTCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATg                         >  2:538487/1‑140 (MQ=255)                                                                                                          gtgtCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTg                     >  2:44917/1‑140 (MQ=255)                                                                                                          gtgtCTTCTTCTCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTg                     >  1:1534161/1‑140 (MQ=255)                                                                                                              cttcttctCACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGatat                 <  2:727673/140‑1 (MQ=255)                                                                                                                   tctcACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCg            >  1:862115/1‑140 (MQ=255)                                                                                                                   tctcACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCg            <  2:932811/140‑1 (MQ=255)                                                                                                                   tctcACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCg            <  1:538487/140‑1 (MQ=255)                                                                                                                     tcACGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGtt          >  2:1143153/1‑140 (MQ=255)                                                                                                                       aCGCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTaa        >  1:1614489/1‑140 (MQ=255)                                                                                                                         gCTTTTACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAAtt      >  1:129054/1‑140 (MQ=255)                                                                                                                            tttACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCAGCGTTAATTTGa   >  1:735784/1‑140 (MQ=255)                                                                                                                             ttACGTTGGCAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGAc  <  2:1202792/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                   CGTATGTGCTGGTTGGTGCATTCGTTGTCGGGATGTTGCTGACGCCGCCGGATGTCTTCTCGCAAACGCTGTTGGCGATCCCGATGTACTGTCTGTTTGAAATCGGTGTCTTCTTCTCACGCTTTTACGTTGGTAAAGGGCGAAATCGGGAAGAGGAAAACGACGCTGAAGCAGAAAGCGAAAAAACTGAAGAATAAATTCAACCGCCCGTCAGGGCGGTTGTCATATGGAGTACAGGATGTTTGATATCGGCGTTAATTTGAC  >  NC_000913/4023316‑4023579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑