Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,393,067	T→C	H334R (CAC→CGC)	queG ←	epoxyqueuosine reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,393,067	0	T	C	100.0%	273.3 / NA	77	H334R (CAC→CGC)	queG	epoxyqueuosine reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/33);  total (44/33)

AGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGA‑‑CGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAA  >  NC_000913/4392930‑4393203                                                                                                                                            |                                                                                                                                         aGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGA‑‑CGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGgcgc                                                                                                                                        >  1:696127/1‑140 (MQ=255) aGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGA‑‑CGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGgcgc                                                                                                                                        >  1:969169/1‑140 (MQ=255) aGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGA‑‑CGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGgcgc                                                                                                                                        >  2:745567/1‑140 (MQ=255)   gCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGACGCGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGgcgc                                                                                                                                        >  1:873364/1‑140 (MQ=255)   gCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGA‑‑CGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTc                                                                                                                                      <  2:937143/140‑1 (MQ=255)   gCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGA‑‑CGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTc                                                                                                                                      >  2:438006/1‑140 (MQ=255)   gCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGA‑‑CGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTc                                                                                                                                      >  1:1262491/1‑140 (MQ=255)          gcgGTAACCCTTTTTCAATCACCCGAACCAGA‑‑CGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTTATCGAGAAGTGGGCGCTCACCTTTa                                                                                                                               <  2:1492725/140‑1 (MQ=255)             gTAACCCTTTTTCAATCACCCGAACCAGA‑‑CGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGa                                                                                                                            >  2:860888/1‑140 (MQ=255)             gTAACCCTTTTTCAATCACCCGAACCAGA‑‑CGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGa                                                                                                                            >  1:660376/1‑140 (MQ=255)                    tttttCAATCACCCGAACCAGA‑‑CGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCa                                                                                                                     >  2:878145/1‑140 (MQ=255)                      tttCAATCACCCGAACCAGA‑‑CGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAgc                                                                                                                   >  1:944613/1‑140 (MQ=255)                       ttCAATCACCCGAACCAGA‑‑CGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAgcg                                                                                                                  <  2:1002295/140‑1 (MQ=255)                       ttCAATCACCCGAACCAGA‑‑CGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAgcg                                                                                                                  <  1:321797/140‑1 (MQ=255)                              aCCCGAACCAGA‑‑CGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCaa                                                                                                           >  1:1020695/1‑140 (MQ=255)                                 cGAACCAGA‑‑CGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAAt                                                                                                        <  2:969169/140‑1 (MQ=255)                                 cGAACCAGA‑‑CGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAAt                                                                                                        >  1:309639/1‑140 (MQ=255)                                     ccAGA‑‑CGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGtt                                                                                                    <  1:517763/140‑1 (MQ=255)                                        gA‑‑CGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCa                                                                                                 >  2:1318598/1‑140 (MQ=255)                                            cGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATc                                                                                               >  2:719383/1‑140 (MQ=255)                                            cGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATc                                                                                               <  2:696127/140‑1 (MQ=255)                                                gTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCaa                                                                                           >  1:440880/1‑140 (MQ=255)                                                gTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCaa                                                                                           >  2:556593/1‑140 (MQ=255)                                                gTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCaa                                                                                           <  1:923495/140‑1 (MQ=255)                                                 ttttttCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAg                                                                                          >  2:368808/1‑140 (MQ=255)                                                      tCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCa                                                                                     <  2:440880/140‑1 (MQ=255)                                                      tCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCa                                                                                     <  1:719383/140‑1 (MQ=255)                                                      tCGGCAATTGCACTCCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCa                                                                                     <  2:1020695/140‑1 (MQ=255)                                                         gCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTg                                                                                  <  1:781063/140‑1 (MQ=255)                                                           aaTTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGcc                                                                                <  2:343184/140‑1 (MQ=255)                                                              tGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTaa                                                                             >  2:1061686/1‑140 (MQ=255)                                                                 aCTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGc                                                                          <  1:860888/140‑1 (MQ=255)                                                                      gACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACgg                                                                     <  1:1012242/140‑1 (MQ=255)                                                                      gACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACgg                                                                     <  1:129849/140‑1 (MQ=255)                                                                      gACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACgg                                                                     <  2:309639/140‑1 (MQ=255)                                                                       aCTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGc                                                                    >  1:1087075/1‑140 (MQ=255)                                                                        cTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCg                                                                   <  1:902804/140‑1 (MQ=255)                                                                               cGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTAc                                                            >  1:120771/1‑140 (MQ=255)                                                                               cGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTAc                                                            <  1:32221/140‑1 (MQ=255)                                                                                gCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACg                                                           <  1:1318598/140‑1 (MQ=255)                                                                                gCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACg                                                           <  2:1049732/140‑1 (MQ=255)                                                                                 cATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGc                                                          <  1:1437193/140‑1 (MQ=255)                                                                                  aTTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGTCTAATGCTACGGCGATATTACGCa                                                         <  2:944613/140‑1 (MQ=255)                                                                                  aTTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCa                                                         <  1:692557/140‑1 (MQ=255)                                                                                  aTTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCa                                                         >  2:1069830/1‑140 (MQ=255)                                                                                  aTTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCa                                                         >  2:767540/1‑140 (MQ=255)                                                                                     tCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGcc                                                      >  1:306645/1‑140 (MQ=255)                                                                                      cGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCa                                                     <  1:556593/140‑1 (MQ=255)                                                                                          tctctATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGc                                                 >  1:468984/1‑140 (MQ=255)                                                                                            tctATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGcc                                                      <  2:452961/133‑1 (MQ=255)                                                                                            tctATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGcc                                                      >  1:452961/1‑133 (MQ=255)                                                                                            tctATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAg                                               >  2:768124/1‑140 (MQ=255)                                                                                            tctATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAg                                               >  1:1201846/1‑140 (MQ=255)                                                                                              tATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGt                                             >  2:906965/1‑140 (MQ=255)                                                                                              tATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGt                                             <  1:878145/140‑1 (MQ=255)                                                                                                   gctgcGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCa                                        >  2:1091603/1‑140 (MQ=255)                                                                                                     tgcGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAAt                                      >  2:1196374/1‑140 (MQ=255)                                                                                                     tgcGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAAt                                      >  2:1531110/1‑140 (MQ=255)                                                                                                      gcgcAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATa                                     >  1:1267499/1‑140 (MQ=255)                                                                                                      gcgcAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATa                                     >  1:279622/1‑140 (MQ=255)                                                                                                      gcgcAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATa                                     >  1:1603086/1‑140 (MQ=255)                                                                                                      gcgcAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATa                                     >  1:1283376/1‑140 (MQ=255)                                                                                                      gcgcAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATa                                     >  1:1292583/1‑140 (MQ=255)                                                                                                        gcAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCGAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATacg                                   >  2:88881/1‑140 (MQ=255)                                                                                                           aTCGCCCACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATacgacg                                <  1:1531110/140‑1 (MQ=255)                                                                                                                ccACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCg                           <  2:279622/140‑1 (MQ=255)                                                                                                                 cACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCGAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGc                          <  1:88881/140‑1 (MQ=255)                                                                                                                 cACGCTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGc                          <  1:1028763/140‑1 (MQ=255)                                                                                                                     cTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGAt                      <  2:468984/140‑1 (MQ=255)                                                                                                                     cTATGTGCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGAt                      <  2:120771/140‑1 (MQ=255)                                                                                                                        tgtgCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCt                   >  2:147752/1‑140 (MQ=255)                                                                                                                         gtgCTCATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCtt                  >  1:1235307/1‑140 (MQ=255)                                                                                                                              cATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTg             >  1:354383/1‑140 (MQ=255)                                                                                                                              cATCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTg             >  1:597421/1‑140 (MQ=255)                                                                                                                                tCGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGAc           >  1:895966/1‑140 (MQ=255)                                                                                                                                 cGAGAAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACt          >  1:564474/1‑140 (MQ=255)                                                                                                                                    gaAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACtttt       >  2:68795/1‑140 (MQ=255)                                                                                                                                    gaAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACtttt       >  1:1344937/1‑140 (MQ=255)                                                                                                                                    gaAGTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACtttt       >  2:978863/1‑140 (MQ=255)                                                                                                                                       gTGGGCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTaaa    >  2:1553485/1‑140 (MQ=255)                                                                                                                                         gggCGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTaaaaa  >  2:1046004/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                                         AGGCGTCACGCGGTAACCCTTTTTCAATCACCCGAACCAGA‑‑CGCTGTTTTTTCGGCAATTGCACTTCGACTATGCACGCATTTCGTCTCTCTATTTGCTGCGCAATCGCCCACGCTATGTGCTCATCGAGAAGTGGGTGCTCACCTTTACGACTTTCCAGCGCTGTCAAAATCGTTTCATCCCAAGGGGCATTGCCTAATGCTACGGCGATATTACGCAGCCAACGCAGGTGACCAATACGACGAATCGCCGATCCTTCCGTGACTTTTAAAAA  >  NC_000913/4392930‑4393203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑