Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,466,090	T→C	T31A (ACT→GCT)	treB ←	trehalose‑specific PTS enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,466,090	0	T	C	100.0%	315.9 / NA	91	T31A (ACT→GCT)	treB	trehalose‑specific PTS enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/48);  total (43/48)

ATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAA  >  NC_000913/4465953‑4466220                                                                                                                                          |                                                                                                                                   aTAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTAACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCaa                                                                                                                                  >  2:289298/1‑140 (MQ=255)     tAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACa                                                                                                                              >  1:1546247/1‑140 (MQ=255)     tAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACa                                                                                                                              >  2:1475135/1‑140 (MQ=255)      aaTCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAg                                                                                                                             <  2:288433/140‑1 (MQ=255)            ccACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTc                                                                                                                       >  2:1445319/1‑140 (MQ=255)              aCGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCAc                                                                                                                     >  1:997455/1‑140 (MQ=255)               cGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCAcc                                                                                                                    >  1:1341235/1‑140 (MQ=255)                    gTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTc                                                                                                                 <  1:1356024/138‑1 (MQ=255)                    gTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTc                                                                                                                 >  2:1356024/1‑138 (MQ=255)                      gCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCaa                                                                                                             >  1:909052/1‑140 (MQ=255)                          aTCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCAc                                                                                                                     >  2:19389/1‑128 (MQ=255)                          aTCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCAc                                                                                                                     <  1:19389/128‑1 (MQ=255)                          aTCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATAtt                                                                                                         >  2:1646054/1‑140 (MQ=255)                          aTCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATAtt                                                                                                         >  2:496764/1‑140 (MQ=255)                          aTCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATAtt                                                                                                         >  2:989405/1‑140 (MQ=255)                               caccTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCa                                                                                                                      <  1:493615/122‑1 (MQ=255)                               caccTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCa                                                                                                                      >  2:493615/1‑122 (MQ=255)                               caccTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCcgc                                                                                                    <  1:496764/140‑1 (MQ=255)                                    gAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGcgcccg                                                                                               <  2:1341235/140‑1 (MQ=255)                                       aTTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCccgccg                                                                                            >  1:678113/1‑140 (MQ=255)                                        ttGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGa                                                                                           <  1:401559/140‑1 (MQ=255)                                        ttGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGa                                                                                           <  2:678113/140‑1 (MQ=255)                                        ttGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGa                                                                                           <  2:909052/140‑1 (MQ=255)                                         tGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGAc                                                                                          <  2:490916/140‑1 (MQ=255)                                         tGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGAc                                                                                          <  1:1445319/140‑1 (MQ=255)                                         tGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGAc                                                                                          <  2:1500062/140‑1 (MQ=255)                                           cccGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGAc                                                                                          <  2:672712/138‑1 (MQ=255)                                           cccGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGAc                                                                                          >  1:672712/1‑138 (MQ=255)                                             cGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGt                                                                                      >  1:1346563/1‑140 (MQ=255)                                                cATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTtca                                                                                   >  2:1346361/1‑140 (MQ=255)                                                       gAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAAcc                                                                            <  1:1646054/140‑1 (MQ=255)                                                          aCAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAAc                                                                             >  2:539615/1‑136 (MQ=255)                                                          aCAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAAc                                                                             <  1:539615/136‑1 (MQ=255)                                                          aCAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACcgat                                                                         <  2:1236065/140‑1 (MQ=255)                                                          aCAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACcgat                                                                         >  1:1134872/1‑140 (MQ=255)                                                             gCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACcgatcga                                                                      >  1:62206/1‑140 (MQ=255)                                                                   cACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCg                                                                <  1:1330720/140‑1 (MQ=255)                                                                    gccATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGGGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGt                                                               <  2:782551/139‑1 (MQ=255)                                                                           ggAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGttt                                                        >  2:216347/1‑140 (MQ=255)                                                                            gAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTa                                                       <  1:1346361/140‑1 (MQ=255)                                                                             aGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTAt                                                      >  2:1009130/1‑140 (MQ=255)                                                                             aGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTAt                                                      >  1:717248/1‑140 (MQ=255)                                                                               ttGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTAttt                                                    >  1:566852/1‑140 (MQ=255)                                                                                tGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGatat                                                                   <  1:1546535/124‑1 (MQ=255)                                                                                tGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGatat                                                                   >  2:1546535/1‑124 (MQ=255)                                                                                tGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTAtttt                                                   >  1:963496/1‑140 (MQ=255)                                                                                tGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTAtttt                                                   >  1:581947/1‑140 (MQ=255)                                                                                tGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTAtttt                                                   >  1:806369/1‑140 (MQ=255)                                                                                tGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTAtttt                                                   >  2:1329651/1‑140 (MQ=255)                                                                                  cTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGc                                                 >  2:1630446/1‑140 (MQ=255)                                                                                  cTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGc                                                 >  1:1630269/1‑140 (MQ=255)                                                                                  cTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGc                                                 >  1:950730/1‑140 (MQ=255)                                                                                  cTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGc                                                 >  2:1419940/1‑140 (MQ=255)                                                                                  cTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATC‑ACCGATCGATATCCGTTTGGTTTAtttttcc                                                >  2:1350330/1‑137 (MQ=255)                                                                                  cTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCATCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGc                                                 >  2:1229046/1‑140 (MQ=255)                                                                                   tCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCt                                                <  2:566852/140‑1 (MQ=255)                                                                                   tCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCt                                                <  1:289298/140‑1 (MQ=255)                                                                                    cAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCtc                                               <  2:950730/140‑1 (MQ=255)                                                                                     aaTTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCATCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCtca                                              <  1:1229046/140‑1 (MQ=255)                                                                                           tttcGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATaa                                        <  1:1475135/140‑1 (MQ=255)                                                                                           tttcGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATaa                                        <  1:1630446/140‑1 (MQ=255)                                                                                             tcGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGATCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAg                                      <  1:1009130/140‑1 (MQ=255)                                                                                                     gCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATgg                              >  2:913424/1‑140 (MQ=255)                                                                                                           gCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATg                        <  2:963496/140‑1 (MQ=255)                                                                                                           gCCGGTTGGTTGAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATg                        >  1:194696/1‑140 (MQ=255)                                                                                                               gttggttgAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGAt                         >  1:798722/1‑135 (MQ=255)                                                                                                               gttggttgAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGAt                         <  2:798722/135‑1 (MQ=255)                                                                                                               gttggttgAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACAt                    >  2:468521/1‑140 (MQ=255)                                                                                                                      gAGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTttggt             <  1:649408/140‑1 (MQ=255)                                                                                                                       aGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTttggtt            <  2:1134872/140‑1 (MQ=255)                                                                                                                       aGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTttggtt            <  2:1330949/140‑1 (MQ=255)                                                                                                                       aGGACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTttggtt            <  2:1346563/140‑1 (MQ=255)                                                                                                                         gACAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTttggttgg          <  1:1559596/140‑1 (MQ=255)                                                                                                                          aCAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGc         >  1:1238854/1‑140 (MQ=255)                                                                                                                           cAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCt        <  1:1329651/140‑1 (MQ=255)                                                                                                                           cAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCt        <  1:1366612/140‑1 (MQ=255)                                                                                                                           cAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCt        <  2:997455/140‑1 (MQ=255)                                                                                                                           cAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCt        <  1:1419940/140‑1 (MQ=255)                                                                                                                           cAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCt        <  1:84716/140‑1 (MQ=255)                                                                                                                           cAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCt        <  2:194696/140‑1 (MQ=255)                                                                                                                           cAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCt        <  2:1630269/140‑1 (MQ=255)                                                                                                                           cAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCt        <  2:1012727/140‑1 (MQ=255)                                                                                                                           cAAAGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCt        <  2:1546247/140‑1 (MQ=255)                                                                                                                            aaaGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTg       <  2:1238854/140‑1 (MQ=255)                                                                                                                             aaGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGc      <  1:989405/140‑1 (MQ=255)                                                                                                                             aaGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGc      <  1:1337257/140‑1 (MQ=255)                                                                                                                             aaGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGc      <  2:717248/140‑1 (MQ=255)                                                                                                                              aGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTttggtt            >  1:1388837/1‑133 (MQ=255)                                                                                                                              aGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTttggtt            <  2:1388837/133‑1 (MQ=255)                                                                                                                              aGCGTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCa     >  2:331027/1‑140 (MQ=255)                                                                                                                                 gTAGGCGAGCAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGaa  >  1:798574/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                   ATAGTAATCACCCACGTTGGTGCCAATCACCACCTGAAATTGCCCGGCATTGGTGAAACAGCCTTTCACCATAGGGAGTTGCTCAATTTCTTTCGGTCTGGCATTGGCCGGTTGGTTGAGGACAAAGCGTAGGCGAGTAATACAGTGGCTCACCGTCGCAATATTGCCGCGCCCGCCGACCAGTTCAATCAACCGATCGATATCCGTTTGGTTTATTTTGCTCATCATAAAGCCCCATGGCAGATGACATTTTTGGTTGGCTGCAGAA  >  NC_000913/4465953‑4466220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑