Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 759,856 T→C V384A (GTA→GCA)  sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913759,8560TC100.0% 278.7 / NA 79V384A (GTA→GCA) sucAsubunit of E1(0) component of 2‑oxoglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/41);  total (38/41)

ACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGG  >  NC_000913/759725‑759987
                                                                                                                                   |                                                                                                                                   
aCTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCg                                                                                                                             >  1:1400520/1‑140 (MQ=255)
 cTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGt                                                                                                                            <  1:1130990/140‑1 (MQ=255)
  ttGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGtt                                                                                                                           >  2:1519950/1‑140 (MQ=255)
  ttGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGtt                                                                                                                           >  1:252163/1‑140 (MQ=255)
     atgaGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATc                                                                                                                        >  2:37710/1‑140 (MQ=255)
     atgaGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATc                                                                                                                        >  2:1593824/1‑140 (MQ=255)
     atgaGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATc                                                                                                                        >  2:1352236/1‑140 (MQ=255)
      tgaGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATca                                                                                                                       <  2:600178/140‑1 (MQ=255)
          ccGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATcaacaa                                                                                                                   >  1:517823/1‑140 (MQ=255)
          ccGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATcaacaa                                                                                                                   >  2:614865/1‑140 (MQ=255)
          ccGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATcaacaa                                                                                                                   >  1:1236752/1‑140 (MQ=255)
          ccGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATcaacaa                                                                                                                   >  2:989880/1‑140 (MQ=255)
            gAGCAGCAACAAAGTGCTGCCAATCACAATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAAcc                                                                                                                 >  1:1634619/1‑140 (MQ=255)
                agcaACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAggt                                                                                                             <  2:840155/140‑1 (MQ=255)
                         gTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTcac                                                                                                    <  2:1236752/140‑1 (MQ=255)
                         gTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTcac                                                                                                    <  1:1593824/140‑1 (MQ=255)
                         gTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTcac                                                                                                    <  1:1612767/140‑1 (MQ=255)
                          tgctgcCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTcacc                                                                                                   <  2:1400520/140‑1 (MQ=255)
                          tgctgcCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTcacc                                                                                                   <  1:893332/140‑1 (MQ=255)
                          tgctgcCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTcacc                                                                                                   >  1:32561/1‑140 (MQ=255)
                            ctgcCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTcaccac                                                                                                 >  2:768842/1‑140 (MQ=255)
                            ctgcCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTcaccac                                                                                                 >  2:171369/1‑140 (MQ=255)
                            ctgcCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTcaccac                                                                                                 <  1:1352236/140‑1 (MQ=255)
                            ctgcCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTcaccac                                                                                                 >  1:517204/1‑140 (MQ=255)
                               ccAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTcac                                                                                                    >  1:848120/1‑134 (MQ=255)
                               ccAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTcac                                                                                                    <  2:848120/134‑1 (MQ=255)
                                    cACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATc                                                                                         <  1:989880/140‑1 (MQ=255)
                                    cACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATc                                                                                         <  1:1519950/140‑1 (MQ=255)
                                             cGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATg                                                                                >  2:1389855/1‑140 (MQ=255)
                                              ggTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGc                                                                               <  2:517204/140‑1 (MQ=255)
                                                tGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGccc                                                                             >  2:402987/1‑140 (MQ=255)
                                                   cgccgcAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGtt                                                                          >  1:514955/1‑140 (MQ=255)
                                                   cgccgcAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGtt                                                                          >  2:1529056/1‑140 (MQ=255)
                                                    gccgcAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTc                                                                         >  2:457378/1‑140 (MQ=255)
                                                     ccgcAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCt                                                                        <  2:303073/140‑1 (MQ=255)
                                                     ccgcAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCt                                                                        >  2:475811/1‑140 (MQ=255)
                                                     ccgcAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCt                                                                        >  2:1449051/1‑140 (MQ=255)
                                                     ccgcAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCt                                                                        <  2:44516/140‑1 (MQ=255)
                                                      cgcAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTa                                                                       <  2:1634619/140‑1 (MQ=255)
                                                        cAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACg                                                                     <  2:517823/140‑1 (MQ=255)
                                                        cAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACg                                                                     <  2:804714/140‑1 (MQ=255)
                                                        cAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACg                                                                     <  2:881594/140‑1 (MQ=255)
                                                          gTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACg                                                                     <  1:838649/138‑1 (MQ=255)
                                                          gTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACg                                                                     >  2:838649/1‑138 (MQ=255)
                                                          gTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGcc                                                                   <  2:32561/140‑1 (MQ=255)
                                                             aCCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCgta                                                                <  1:768842/140‑1 (MQ=255)
                                                             aCCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCgta                                                                <  2:1615665/140‑1 (MQ=255)
                                                             aCCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAAGCCGCTGGATGCCCGTTCTACGCCgta                                                                <  2:252163/140‑1 (MQ=255)
                                                                   cAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCgtactgtac                                                          <  1:457378/140‑1 (MQ=255)
                                                                   cAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCgtactgtac                                                          >  1:813167/1‑140 (MQ=255)
                                                                     gggCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCgta                                                                >  2:825449/1‑132 (MQ=255)
                                                                     gggCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCgta                                                                <  1:825449/132‑1 (MQ=255)
                                                                       gCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGat                                                      <  1:1064731/140‑1 (MQ=255)
                                                                       gCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGat                                                      <  1:1529056/140‑1 (MQ=255)
                                                                       gCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGat                                                      <  2:514955/140‑1 (MQ=255)
                                                                            gTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCgta                                                                <  2:266533/125‑1 (MQ=255)
                                                                            gTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCgta                                                                >  1:266533/1‑125 (MQ=255)
                                                                            gTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCgg                                                 <  2:17939/140‑1 (MQ=255)
                                                                            gTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCgg                                                 <  1:1611306/140‑1 (MQ=255)
                                                                                       ccTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTc                                      >  1:1592915/1‑140 (MQ=255)
                                                                                       ccTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTc                                      >  1:445582/1‑140 (MQ=255)
                                                                                           aaCATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGc                                  >  1:1073817/1‑140 (MQ=255)
                                                                                                gTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGa                             >  2:579058/1‑140 (MQ=255)
                                                                                                    aaaGCGCGTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGAtttt                         <  1:579058/140‑1 (MQ=255)
                                                                                                          cgTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAgg                                   <  2:1086441/124‑1 (MQ=255)
                                                                                                          cgTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAgg                                   >  1:1086441/1‑124 (MQ=255)
                                                                                                          cgTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAgg                                   >  1:586847/1‑124 (MQ=255)
                                                                                                          cgTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAgg                                   <  2:586847/124‑1 (MQ=255)
                                                                                                          cgTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGa                             <  2:799145/130‑1 (MQ=255)
                                                                                                          cgTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGa                             >  1:799145/1‑130 (MQ=255)
                                                                                                          cgTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGt                   >  1:789514/1‑140 (MQ=255)
                                                                                                          cgTGGTTATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGt                   >  1:1233698/1‑140 (MQ=255)
                                                                                                               ttATGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAAcg              <  2:1073817/140‑1 (MQ=255)
                                                                                                                 aTGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAAcgcg            <  1:772031/140‑1 (MQ=255)
                                                                                                                 aTGAAGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAAcgcg            <  1:614865/140‑1 (MQ=255)
                                                                                                                    aaGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGAc         <  1:402987/140‑1 (MQ=255)
                                                                                                                    aaGTTGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGAc         <  1:1449051/140‑1 (MQ=255)
                                                                                                                       ttGGCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGAt      >  1:1327304/1‑140 (MQ=255)
                                                                                                                         ggCGGTACGGCACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATcc    >  2:840578/1‑140 (MQ=255)
                                                                                                                         ggCGGTACGGCACGTATCGTTATCAACAAACAGGTTGGTTTTACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATcc    >  2:563474/1‑140 (MQ=255)
                                                                                                                           cggtacggcaCGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCgg  >  2:366765/1‑140 (MQ=255)
                                                                                                                                   |                                                                                                                                   
ACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGG  >  NC_000913/759725‑759987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑