Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	775,617	C→T	A155A (GCC→GCT)	tolQ →	Tol‑Pal system protein TolQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	775,617	0	C	T	100.0%	200.1 / NA	56	A155A (GCC→GCT)	tolQ	Tol‑Pal system protein TolQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (30/26);  total (30/26)

CGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCA  >  NC_000913/775480‑775742                                                                                                                                          |                                                                                                                              cgTGCTATGCGTAGCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGctct                                                                                                                             <  2:367838/140‑1 (MQ=255)      tATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCgggg                                                                                                                        <  1:377182/140‑1 (MQ=255)           gTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTa                                                                                                                   <  2:905201/140‑1 (MQ=255)             aTCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTaaa                                                                                                                 <  2:324076/140‑1 (MQ=255)             aTCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTaaa                                                                                                                 >  2:653779/1‑140 (MQ=255)                  cATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAg                                                                                                            >  2:413093/1‑140 (MQ=255)                  cATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTAGCTCCATCAGCCCGTATATTGGTCTGTTTGGTATGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAg                                                                                                            <  2:1443578/140‑1 (MQ=255)                    tGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCa                                                                                                          <  2:1578565/140‑1 (MQ=255)                      aaCCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAac                                                                                                        <  1:1119860/140‑1 (MQ=255)                      aaCCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAac                                                                                                        <  2:1212110/140‑1 (MQ=255)                       aCCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAaca                                                                                                       <  2:233219/140‑1 (MQ=255)                       aCCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAaca                                                                                                       <  1:653779/140‑1 (MQ=255)                       aCCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAaca                                                                                                       <  2:1330852/140‑1 (MQ=255)                          gTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTaaa                                                                                                                 <  2:1605843/127‑1 (MQ=255)                          gTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTaaa                                                                                                                 >  1:1605843/1‑127 (MQ=255)                          gTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTg                                                                                                    >  1:719540/1‑140 (MQ=255)                                      aTCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTgcg                                                                                        <  1:413093/140‑1 (MQ=255)                                        cTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGcc                                                                                      <  2:719540/140‑1 (MQ=255)                                         tGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGccc                                                                                     >  1:1359664/1‑140 (MQ=255)                                             aaCGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTa                                                                                 <  2:367673/140‑1 (MQ=255)                                              aCGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTAt                                                                                <  1:315026/140‑1 (MQ=255)                                                 cacaTTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGc                                                                             >  1:471694/1‑140 (MQ=255)                                                  acaTTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCa                                                                            <  2:511853/140‑1 (MQ=255)                                                        cGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGccc                                                                                     >  1:249759/1‑125 (MQ=255)                                                        cGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGccc                                                                                     <  2:249759/125‑1 (MQ=255)                                                           tCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTg                                                                   >  1:939022/1‑140 (MQ=255)                                                                  tACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAg                                                                        >  2:290891/1‑128 (MQ=255)                                                                  tACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAg                                                                        <  1:290891/128‑1 (MQ=255)                                                                       ttGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGTATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCg                                                                      >  2:194448/1‑125 (MQ=255)                                                                       ttGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGTATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCg                                                                      <  1:194448/125‑1 (MQ=255)                                                                       ttGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCa                                                       >  2:1235800/1‑140 (MQ=255)                                                                            tCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTgg                                                  >  2:1599504/1‑140 (MQ=255)                                                                                tCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTg                                              >  2:563994/1‑140 (MQ=255)                                                                                              ggTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATccc                                >  1:654942/1‑140 (MQ=255)                                                                                              ggTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATccc                                >  2:103948/1‑140 (MQ=255)                                                                                              ggTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATccc                                >  1:716388/1‑140 (MQ=255)                                                                                               gTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGtt                                            <  2:1401801/127‑1 (MQ=255)                                                                                               gTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGtt                                            >  1:1401801/1‑127 (MQ=255)                                                                                               gTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCg                               >  1:630897/1‑140 (MQ=255)                                                                                               gTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCg                               >  2:672438/1‑140 (MQ=255)                                                                                                tCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCgg                              <  1:35729/140‑1 (MQ=255)                                                                                                tCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCgg                              <  1:1599504/140‑1 (MQ=255)                                                                                                    tttGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGt                          >  1:527300/1‑140 (MQ=255)                                                                                                    tttGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGt                          >  2:1006455/1‑140 (MQ=255)                                                                                                      tGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTa                        <  1:103948/140‑1 (MQ=255)                                                                                                       ggTACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTAt                       <  2:939022/140‑1 (MQ=255)                                                                                                         tACGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATgg                     >  2:154866/1‑140 (MQ=255)                                                                                                          aCGGTCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGc                    <  1:1235800/140‑1 (MQ=255)                                                                                                              tCTGGGGGATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTAc                >  1:1016549/1‑140 (MQ=255)                                                                                                                  ggggATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAg                           >  2:1431748/1‑125 (MQ=255)                                                                                                                  ggggATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAg                           <  1:1431748/125‑1 (MQ=255)                                                                                                                   gggATCATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCg           >  1:1127007/1‑140 (MQ=255)                                                                                                                        cATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCa      >  1:950695/1‑140 (MQ=255)                                                                                                                        cATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCa      >  1:1331330/1‑140 (MQ=255)                                                                                                                        cATGCACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCa      >  2:815542/1‑140 (MQ=255)                                                                                                                           cccctcttttACCGCTCTGGGGGCGGAAAAAAAAGCAACACTGCAAATGGTTGCCCCCGGTTTCGCAGAGGCGTTGATTGCGACTGCATTTGGTCTGTTTGCCGCTACCCCGGCGTTTAGGGCCTACAACCGCCTCAAcc   <  2:654942/133‑1 (MQ=255)                                                                                                                            cACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCa  >  1:143202/1‑140 (MQ=255)                                                                                                                            cACGCCTTTATCGCTCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCa  >  1:1225084/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                              CGTGCTATGCGTATCTCCATGAACCGTGAACTTGAAAATCTGGAAACGCACATTCCGTTCCTCGGTACGGTTGGCTCCATCAGCCCGTATATTGGTCTGTTTGGTACGGTCTGGGGGATCATGCACGCCTTTATCGCCCTCGGGGCGGTAAAACAAGCAACACTGCAAATGGTTGCGCCCGGTATCGCAGAAGCGTTGATTGCGACTGCAATTGGTCTGTTTGCCGCTATCCCGGCAGTTATGGCCTACAACCGCCTCAACCA  >  NC_000913/775480‑775742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑