Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	80,258	A→G	F203L (TTT→CTT)	leuC ←	3‑isopropylmalate dehydratase subunit LeuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	80,258	0	A	G	100.0%	237.0 / NA	67	F203L (TTT→CTT)	leuC	3‑isopropylmalate dehydratase subunit LeuC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (39/28);  total (39/28)

TGCAGACGGCCTTTGACATAGTTAAAGGTGGTTTCGTCCGGTGCAACCAGACCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAG  >  NC_000913/80122‑80393                                                                                                                                         |                                                                                                                                        tGCAGACGGCCTTTGACATAGTTAAAGGTGGTTTCGTCCGGTGCAACCAGACCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTc                                                                                                                                      <  2:735388/140‑1 (MQ=255)  gCAGACGGCCTTTGACATAGTTAAAGGTGGTTTCGTCCGGTGCAACCAGACCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTcc                                                                                                                                     >  1:1350070/1‑140 (MQ=255)       cGGCCTTTGACATAGTTAAAGGTGGTTTCGTCCGGTGCAACCAGACCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCcaccac                                                                                                                                <  2:198157/140‑1 (MQ=255)       cGGCCTTTGACATAGTTAAAGGTGGTTTCGTCCGGTGCAACCAGACCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCcaccac                                                                                                                                >  1:423546/1‑140 (MQ=255)          ccTTTGACATAGTTAAAGGTGGTTTCGTCCGGTGCAACCAGACCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATg                                                                                                                             <  1:88588/140‑1 (MQ=255)                    aGTTAAAGGTGGTTTCGTCCGGTGCAACCAGACCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTgccg                                                                                                                   >  2:1145669/1‑140 (MQ=255)                      ttAAAGGTGGTTTCGTCCGGTGCAACCAGACCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTgccgcc                                                                                                                 >  1:1143384/1‑140 (MQ=255)                               gtTTCGTCCGGTGCAACCAGACCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTg                                                                                                               <  2:820669/133‑1 (MQ=255)                               gtTTCGTCCGGTGCAACCAGACCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTg                                                                                                               >  1:820669/1‑133 (MQ=255)                               gtTTCGTCCGGTGCAACCAGACCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTAcc                                                                                                        >  1:1465280/1‑140 (MQ=255)                               gtTTCGTCCGGTGCAACCAGACCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTAcc                                                                                                        >  2:341493/1‑140 (MQ=255)                                tttCGTCCGGTGCAACCAGACCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTGGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCg                                                                                                       >  2:1539910/1‑140 (MQ=255)                                tttCGTCCGGTGCAACCAGACCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCg                                                                                                       >  2:934320/1‑140 (MQ=255)                                tttCGTCCGGTGCAACCAGACCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCg                                                                                                       >  1:865978/1‑140 (MQ=255)                                   cGTCCGGTGCAACCAGACCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGtt                                                                                                    <  1:934320/140‑1 (MQ=255)                                       cGGTGCAACCAGACCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTAc                                                                                                >  1:1244258/1‑140 (MQ=255)                                            cAACCAGACCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATa                                                                                           <  1:922828/140‑1 (MQ=255)                                               ccAGACCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAAtt                                                                                        <  2:976379/140‑1 (MQ=255)                                                   aCCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCa                                                                                    >  1:78289/1‑140 (MQ=255)                                                   aCCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCa                                                                                    >  2:1201530/1‑140 (MQ=255)                                                   aCCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCa                                                                                    >  2:832571/1‑140 (MQ=255)                                                   aCCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCa                                                                                    <  2:590110/140‑1 (MQ=255)                                                        cTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCGCGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCa                                                                                    <  2:1626995/135‑1 (MQ=255)                                                        cTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCGCGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCa                                                                                    >  1:1626995/1‑135 (MQ=255)                                                                  cATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGat                                                                             >  2:20437/1‑132 (MQ=255)                                                                  cATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGat                                                                             <  1:20437/132‑1 (MQ=255)                                                                             ccATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGccc                                                          >  1:683721/1‑140 (MQ=255)                                                                               atatTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCgg                                                        <  2:1615444/140‑1 (MQ=255)                                                                                    gCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCgg                                                   <  1:1201530/140‑1 (MQ=255)                                                                                       caGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCtt                                                >  2:325993/1‑140 (MQ=255)                                                                                            tCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCt                                                 <  1:278262/134‑1 (MQ=255)                                                                                            tCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCt                                                 >  2:278262/1‑134 (MQ=255)                                                                                            tCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGccc                                           >  1:7988/1‑140 (MQ=255)                                                                                            tCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGccc                                           >  1:1418360/1‑140 (MQ=255)                                                                                             cATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCt                                          <  2:7988/140‑1 (MQ=255)                                                                                               tACNACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGNTAATGCCCGGCGCGGCTTTGCCCTgg                                        >  2:21168/1‑140 (MQ=255)                                                                                               tACGACCTTCCATGCTTAAATCACGGGTTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTgg                                        >  1:1043411/1‑140 (MQ=255)                                                                                                   aCCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTgaagtc                                    <  2:1143384/140‑6 (MQ=255)                                                                                                    ccTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCAGGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTc                                   >  2:1258004/1‑140 (MQ=255)                                                                                                    ccTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTc                                   >  2:977404/1‑140 (MQ=255)                                                                                                    ccTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTc                                   >  2:532634/1‑140 (MQ=255)                                                                                                    ccTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTc                                   >  2:272624/1‑140 (MQ=255)                                                                                                    ccTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTc                                   >  1:1427345/1‑140 (MQ=255)                                                                                                    ccTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTc                                   >  1:1312743/1‑140 (MQ=255)                                                                                                      ttCCATGCTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCaa                                 >  1:1659330/1‑140 (MQ=255)                                                                                                             cTTAAATCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCAt                          <  1:392205/140‑1 (MQ=255)                                                                                                                 aaTCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACTGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGtt                      >  1:1250992/1‑140 (MQ=255)                                                                                                                 aaTCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGtt                      >  1:1589787/1‑140 (MQ=255)                                                                                                                  aTCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGttt                     >  2:846345/1‑140 (MQ=255)                                                                                                                  aTCACGGATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGttt                     >  2:1410530/1‑140 (MQ=255)                                                                                                                       ggATTGCTTCGCCGCAAAGCTCCACCGCATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTgcg                >  2:584030/1‑140 (MQ=255)                                                                                                                       ggATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTgcg                <  1:151906/140‑1 (MQ=255)                                                                                                                       ggATTGCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTgcg                <  2:1589787/140‑1 (MQ=255)                                                                                                                            gCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCAGGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTATCATGGTTTTTGCGCGGcc           <  1:1258004/140‑1 (MQ=255)                                                                                                                            gCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGcc           <  1:272624/140‑1 (MQ=255)                                                                                                                            gCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGcc           <  1:325993/140‑1 (MQ=255)                                                                                                                            gCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGcc           <  1:341493/140‑1 (MQ=255)                                                                                                                            gCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGcc           <  2:1148041/140‑1 (MQ=255)                                                                                                                            gCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGcc           <  2:1161828/140‑1 (MQ=255)                                                                                                                            gCTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGcc           <  2:1418360/140‑1 (MQ=255)                                                                                                                             cTTCGCCGCAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGccc          >  1:967841/1‑140 (MQ=255)                                                                                                                                  ccgcAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCGTGttt     <  2:1350070/140‑1 (MQ=255)                                                                                                                                  ccgcAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGttt     >  2:539162/1‑140 (MQ=255)                                                                                                                                  ccgcAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGttt     <  1:274926/140‑1 (MQ=255)                                                                                                                                  ccgcAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGttt     <  2:683721/140‑1 (MQ=255)                                                                                                                                   cgcAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTc    >  1:347073/1‑140 (MQ=255)                                                                                                                                     cAAAGCTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAg  >  2:728706/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TGCAGACGGCCTTTGACATAGTTAAAGGTGGTTTCGTCCGGTGCAACCAGACCGGCTTTTGCGCCCATTTCGATTGCCATATTGCACAGGGTCATACGACCTTCCATGCTTAAATCACGGATTGCTTCGCCGCAAAACTCCACCACATGCCCGGTGCCGCCTGCGCTACCGGTTTTACCGATAATTGCCAGCACGATATCTTTTGCGGTAATGCCCGGCGCGGCTTTGCCCTGGACTTCAATTTTCATGGTTTTTGCGCGGCCCTGTTTCAG  >  NC_000913/80122‑80393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑