Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	843,979	A→G	L501P (CTA→CCA)	ybiO ←	moderate conductance mechanosensitive channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	843,979	0	A	G	100.0%	292.0 / NA	84	L501P (CTA→CCA)	ybiO	moderate conductance mechanosensitive channel
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (52/32);  total (52/32)

ATGCCCCGGCACCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTAGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACGGTTTTCTGCCCCGCGC  >  NC_000913/843842‑844108                                                                                                                                          |                                                                                                                                  aTGCCCCGGCACCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGgc                                                                                                                                 >  2:330497/1‑140 (MQ=255) aTGCCCCGGCACCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGgc                                                                                                                                 >  2:276252/1‑140 (MQ=255)  tGCCCCGGCACCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGCACGCGTGCGGGCGCTGGGTGgcg                                                                                                                                >  2:707449/1‑140 (MQ=255)    ccccGGCACCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGgcggg                                                                                                                              <  1:276252/140‑1 (MQ=255)    ccccGGCACCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGgcggg                                                                                                                              <  1:330497/140‑1 (MQ=255)    ccccGGCACCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGgcggg                                                                                                                              <  2:97865/140‑1 (MQ=255)     cccGGCACCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGgcgggc                                                                                                                             <  2:1501105/140‑1 (MQ=255)      ccGGCACCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTggcgggcg                                                                                                                            >  1:106902/1‑140 (MQ=255)      ccGGCACCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTggcgggcg                                                                                                                            >  2:671455/1‑140 (MQ=255)       cGGCACCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGgcgggcgg                                                                                                                           <  2:1426193/140‑1 (MQ=255)       cGGCACCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGgcgggcgg                                                                                                                           <  2:1548404/140‑1 (MQ=255)       cGGCACCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGgcgggcgg                                                                                                                           >  2:864351/1‑140 (MQ=255)         gCACCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGcc                                                                                                                         >  1:80923/1‑140 (MQ=255)         gCACCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGcc                                                                                                                         <  2:682356/140‑1 (MQ=255)         gCACCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGcc                                                                                                                         <  1:671455/140‑1 (MQ=255)           aCCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCAt                                                                                                                       >  1:798173/1‑140 (MQ=255)              tGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGaa                                                                                                                    <  1:1029047/140‑1 (MQ=255)               gCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAat                                                                                                                   >  2:1648264/1‑140 (MQ=255)               gCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAat                                                                                                                   >  1:394747/1‑140 (MQ=255)                       tGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAag                                                                                                           >  1:634319/1‑140 (MQ=255)                        ggCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAagc                                                                                                          <  1:1429336/140‑1 (MQ=255)                          cgcgATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAagcca                                                                                                        >  1:838623/1‑140 (MQ=255)                          cgcgATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAagcca                                                                                                        >  2:1317416/1‑140 (MQ=255)                          cgcgATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAagcca                                                                                                        >  1:1065041/1‑140 (MQ=255)                                      gCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGa                                                                                            <  1:1588566/140‑1 (MQ=255)                                         gATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCa                                                                                         >  2:137214/1‑140 (MQ=255)                                           tttCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCaaa                                                                                       >  1:206332/1‑140 (MQ=255)                                           tttCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCaaa                                                                                       <  2:997398/140‑1 (MQ=255)                                           tttCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCaaa                                                                                       <  1:994543/140‑1 (MQ=255)                                           tttCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCaaa                                                                                       <  1:497398/140‑1 (MQ=255)                                           tttCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCaaa                                                                                       >  2:81650/1‑140 (MQ=255)                                           tttCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCaaa                                                                                       <  2:545937/140‑1 (MQ=255)                                           tttCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCaaa                                                                                       <  2:627343/140‑1 (MQ=255)                                           tttCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCaaa                                                                                       <  2:798173/140‑1 (MQ=255)                                           tttCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCaaa                                                                                       <  1:137214/140‑1 (MQ=255)                                                    acacAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGc                                                                              <  2:394747/140‑1 (MQ=255)                                                        aaTCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCaaa                                                                                       <  2:438988/127‑1 (MQ=255)                                                        aaTCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCaaa                                                                                       >  1:438988/1‑127 (MQ=255)                                                        aaTCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCg                                                                          >  1:242648/1‑140 (MQ=255)                                                          tcataggggtggtggaACTGATAATCCCCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGGGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTc                                                                        <  2:1624752/133‑1 (MQ=255)                                                          tCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATAAAACTGGCGAGCACCGTc                                                                        <  2:634319/140‑1 (MQ=255)                                                             tgatggtgatggTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGc                                                                                  <  2:985562/127‑1 (MQ=255)                                                             tgatggtgatggTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGc                                                                                  >  1:985562/1‑127 (MQ=255)                                                             tgatggtgatggTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAg                                                                     >  1:1244249/1‑140 (MQ=255)                                                             tgatggtgatggTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAg                                                                     >  1:50529/1‑140 (MQ=255)                                                             tgatggtgatggTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAg                                                                     >  1:811336/1‑140 (MQ=255)                                                               atggtgatggtACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTc                                                                        <  2:1432676/135‑1 (MQ=255)                                                               atggtgatggtACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTc                                                                        >  1:1432676/1‑135 (MQ=255)                                                               atggtgatggtACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGcc                                                                   >  1:1624752/1‑140 (MQ=255)                                                               atggtgatggtACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGcc                                                                   >  1:1647589/1‑140 (MQ=255)                                                                          aCTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCgaga                                                        >  2:1332128/1‑140 (MQ=255)                                                                            tGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGagaag                                                      >  2:1512666/1‑140 (MQ=255)                                                                                 aTCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGaagaaaag                                                 >  2:1137238/1‑140 (MQ=255)                                                                                   cACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGaagaaaagaa                                               >  2:156831/1‑140 (MQ=255)                                                                                   cACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGaagaaaagaa                                               >  1:991818/1‑140 (MQ=255)                                                                                     ccGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACTGTCCAGCCAACCGCCGAGAAGAAAAGAATg                                             >  2:1014943/1‑140 (MQ=255)                                                                                        ccAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCgaga                                                        <  2:739883/126‑1 (MQ=255)                                                                                        ccAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCgaga                                                        >  1:739883/1‑126 (MQ=255)                                                                                        ccAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGt                                          >  1:1041247/1‑140 (MQ=255)                                                                                        ccAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGt                                          >  1:1261127/1‑140 (MQ=255)                                                                                        ccAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGt                                          >  2:646214/1‑140 (MQ=255)                                                                                        ccAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGt                                          >  1:520524/1‑140 (MQ=255)                                                                                         cAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTg                                         <  1:864351/140‑1 (MQ=255)                                                                                         cAGCGCGTTACGAAACAGCGTAAGCAGGGCACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTg                                         <  1:707449/140‑1 (MQ=255)                                                                                          aGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGc                                        >  2:428398/1‑140 (MQ=255)                                                                                            cgcgTTACGAACCAGCGTAGGCAGGGTACGGGTGCGGGCGCGGGGTGGCGGGCGGCCATGAATATCCGAAGCAAGCCGGTTTTCGACCAACCTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGa                                      <  2:206332/140‑1 (MQ=255)                                                                                            cgcgTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAAt                                              >  2:1408540/1‑132 (MQ=255)                                                                                            cgcgTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAAt                                              <  1:1408540/132‑1 (MQ=255)                                                                                             gcgTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGAt                                     <  1:1137238/140‑1 (MQ=255)                                                                                             cagTTACGAAACGGCGTAAGCGGGGTACGGGTGCGGGCGCGGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATAAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGAt                                     <  2:1463224/138‑1 (MQ=255)                                                                                               gTTACGAAACGGCGTAAGCGGGGTGGGCGTGCGGGCGCGGGGTGGCGGGCGGCCAGGAATATCCGAGGCCGGCCGGTTTTCGACCAAACTGGCGCGCACCGACCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATAc                                   <  2:991818/140‑1 (MQ=255)                                                                                                   cGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGAt                               <  1:911038/140‑1 (MQ=255)                                                                                                   cGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGAt                               <  1:646214/140‑1 (MQ=255)                                                                                                   cGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGAt                               <  1:428398/140‑1 (MQ=255)                                                                                                    gAACCAGCGTAAGCGGGGTACGCGCGCGGGCGTGGGGTGGCGGGAGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATAAAACTGGCGAGCACCGCCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATc                              <  2:50529/140‑1 (MQ=255)                                                                                                    gAAACAGCGTAAGCAGGGTCCGCGTGCGGGCGCTGGGGGGCGGCCGCCCATGAATATCCGAAGCCGGCGGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATc                              <  2:1065041/140‑1 (MQ=255)                                                                                                    gAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCGGAACCCAGCCGTTTTTCGATAAAACTGGGGACCACCGCCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATc                              <  2:80923/140‑1 (MQ=255)                                                                                                           cGTAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGactg                       >  1:1463224/1‑137 (MQ=255)                                                                                                             tAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCt                     >  1:1510400/1‑140 (MQ=255)                                                                                                             tAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCt                     >  2:14244/1‑140 (MQ=255)                                                                                                             tAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCt                     >  1:1240516/1‑140 (MQ=255)                                                                                                             tAAGCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCt                     >  2:112801/1‑140 (MQ=255)                                                                                                                gcggggTGCGCGTGGGGGCGCGGGGGGGGGGGGGGCCATGAATATCCGAAGCCAGCGGTTTTTCAATCAAACTGGAGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACg                  <  2:1240516/137‑1 (MQ=255)                                                                                                                gCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACg                  <  1:81650/140‑1 (MQ=255)                                                                                                                gCAGGGTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACg                  >  2:653657/1‑140 (MQ=255)                                                                                                                aaggggTACGCGGGCGGGCGCGGGGTGGGGGGCGCCCATAAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACg                  <  2:1224548/137‑1 (MQ=255)                                                                                                                 acagggaCGCGTGGGGGGGCTGGGTGGCGGGCGGCCAAGAATACCCGAAGCCAGCGGGTTTTCGATCAAACTGGCAGGCCCCGTCCCGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACgg                 <  2:1335723/134‑1 (MQ=255)                                                                                                                     gTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACGGtttt             >  1:140508/1‑140 (MQ=255)                                                                                                                     gTACGCGTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACGGtttt             >  2:1016655/1‑140 (MQ=255)                                                                                                                          ggTGCGGGCGCGGGGGGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACGGTTTTCTGcc        <  2:106902/139‑1 (MQ=255)                                                                                                                          cgTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACGGTTTTCTgac        >  1:1336797/1‑138 (MQ=255)                                                                                                                          cgTGCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACGGTTTTCTGcc        >  1:1257834/1‑140 (MQ=255)                                                                                                                             gCGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAGCCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACGGTTTTCTGCCCcg     <  2:140508/140‑1 (MQ=255)                                                                                                                              cGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACGGTTTTCTGcccctg    >  1:1224548/1‑138 (MQ=255)                                                                                                                              cGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACGGTTTTCTGCCCcgc    >  2:771190/1‑140 (MQ=255)                                                                                                                              cGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACGGTTTTCTGCCCcgc    >  1:392137/1‑140 (MQ=255)                                                                                                                              cGGGCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACGGTTTTCTGCCCcgc    >  1:24629/1‑140 (MQ=255)                                                                                                                                ggCGCTGGGTGGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACGGTTTTCTGccccttgt  >  1:1335723/1‑136 (MQ=255)                                                                                                                                          |                                                                                                                                  ATGCCCCGGCACCTGCCAGCAATGGCGCGATATTGACGCCGATTTCCGACAACACAATCATGATGGTGATGGTACTGATAATCACCGCCAGCGCGTTACGAAACAGCGTAAGCAGGGTACGCGTGCGGGCGCTGGGTAGCGGGCGGCCATGAATATCCGAAGCCAGCCGGTTTTCGATCAAACTGGCGAGCACCGTCCAGCCAACCGCCGAGAAGAAAAGAATGAGTGCGATACGGATCAGGATATCTACGGTTTTCTGCCCCGCGC  >  NC_000913/843842‑844108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑