Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,114,758	A→G	Y245H (TAT→CAT)	mdtG ←	efflux pump MdtG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,114,758	0	A	G	100.0%	169.5 / NA	48	Y245H (TAT→CAT)	mdtG	efflux pump MdtG
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/24);  total (24/24)

GATCTTTTCGGGTCCGATTCGATCGCCAAGTTTGCCGAGTCGTGGTGCACTTAGCAGAGCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCATGTGCAGCAT  >  NC_000913/1114621‑1114878                                                                                                                                          |                                                                                                                         gATCTTTTCGGGTCCGATTCGATCGCCAAGTTTGCCGAGTCGTGGTGCACTTAGCAGAGCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCa                                                                                                                        >  1:264895/1‑140 (MQ=255)  aTCTTTTCGGGTCCGATTCGATCGCCAAGTTTGCCGAGTCGTGGTGCACTTAGCAGAGCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAg                                                                                                                       >  2:698602/1‑140 (MQ=255)    cTTTTCGGGTCCGATTCGATCGCCAAGTTTGCCGAGTCGTGGGGCACTTAGCAGAGCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCg                                                                                                                     >  1:1346552/1‑140 (MQ=255)       ttCGGGTCCGATTCGATCGCCAAGTTTGCCGAGTCGTGGTGCACTTAGCAGAGCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCa                                                                                                                  >  2:1270420/1‑140 (MQ=255)                      aTCGCCAAGTTTGCCGAGTCGTGGTGCACTTAGCAGAGCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTAACCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTg                                                                                                   >  1:1098699/1‑140 (MQ=255)                           cAAGTTTGCCGAGTCGTGGTGCACTTAGCAGAGCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGccc                                                                                              >  2:1233790/1‑140 (MQ=255)                             tttttGCCGGGTGGGGGTGCCTTTAGAAGGCCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGt                                                                                            <  2:92657/138‑1 (MQ=255)                               tttGCGGAGCGGTGGTCCACTTGGCGGAGCCGCCACGCCTGGCACCGAGGCGATCAGGCCACTGATAAAGGCGACGTTACTGCCGTTGCCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCg                                                                                          <  2:98404/140‑1 (MQ=255)                                     gAGTGGTGGTCCATTAAGCAGAGCCCCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCt                                                                                    <  2:211165/140‑1 (MQ=255)                                       gtgggggtgCATTTAGAAGAGCCGCCCCGCCTGGCCCCGAGGCGATCATGCCACTGATAAGGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTgg                                                                                  <  2:1074797/135‑1 (MQ=255)                                       gacggggtgCACTAAGCAGGCCCCCCCCGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTCCCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTgg                                                                                  <  2:278334/135‑1 (MQ=255)                                        tCGTGGTGCACTTAGCAGAGCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGga                                                                                 >  1:1263549/1‑140 (MQ=255)                                            ggtcCATTTAGCAGACCCCCCCCGCCTGGCCCCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGgatgat                                                                             <  2:25076/140‑1 (MQ=255)                                                  cTTAGCAGAGCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGt                                                                       >  1:876237/1‑140 (MQ=255)                                                     aGCAGAGCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGt                                                                    <  1:583316/140‑1 (MQ=255)                                                       cAGAGCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGtctgtc                                                                  >  1:278334/1‑135 (MQ=255)                                                        agagCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGAc                                                                 >  2:772739/1‑140 (MQ=255)                                                          agCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACaa                                                               >  1:832792/1‑140 (MQ=255)                                                            ccgccACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAAc                                                             >  1:1175616/1‑140 (MQ=255)                                                             cgccACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACa                                                            >  1:32280/1‑140 (MQ=255)                                                               ccACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAgg                                                          >  1:1339341/1‑140 (MQ=255)                                                                  cGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTg                                                       <  1:268802/140‑1 (MQ=255)                                                                  cGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTg                                                       <  1:698602/140‑1 (MQ=255)                                                                            cGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGtt                                             >  1:1353913/1‑140 (MQ=255)                                                                              gggCGATCATGCCACTGAAAAAGGCGACGTTACTGACGTTCCCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTc                                           <  2:244654/139‑1 (MQ=255)                                                                              aGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTc                                           >  1:1064712/1‑140 (MQ=255)                                                                                gCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCgg                                         <  2:876237/140‑1 (MQ=255)                                                                                 cGACCATGCCACTGATAAAGCCGACGTTTCTGACGTTAACCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCggg                                        <  2:1098699/140‑1 (MQ=255)                                                                                   aTCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGtt                                      >  1:1262015/1‑140 (MQ=255)                                                                                             tGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGt                               >  1:649644/1‑137 (MQ=255)                                                                                             tGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGt                               <  2:649644/137‑1 (MQ=255)                                                                                             tGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGAt                            >  1:839614/1‑140 (MQ=255)                                                                                               aTAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGt                          >  1:206787/1‑140 (MQ=255)                                                                                                 aaaGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTca                        <  2:1064712/140‑1 (MQ=255)                                                                                                  aaGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTcac                       <  2:1175616/140‑1 (MQ=255)                                                                                                  aaGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTcac                       <  2:1339341/140‑1 (MQ=255)                                                                                                  aaGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTcac                       <  1:418173/140‑1 (MQ=255)                                                                                                  aaGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTcac                       <  1:1297275/140‑1 (MQ=255)                                                                                                      cGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACt                   >  1:1612463/1‑140 (MQ=255)                                                                                                      cGACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACt                   >  1:448978/1‑140 (MQ=255)                                                                                                       gACGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACtt                  <  2:32280/140‑1 (MQ=255)                                                                                                         cGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTcac                       <  1:1176931/133‑1 (MQ=255)                                                                                                         cGTTACTGACGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTcac                       >  2:1176931/1‑133 (MQ=255)                                                                                                                  cGTTACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCATGTgc       <  2:832792/140‑1 (MQ=255)                                                                                                                    ttACCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCATGTgcag     <  2:894312/140‑1 (MQ=255)                                                                                                                      aCCCGCCAGTTCGCGGTCATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCATGTgcagca   >  2:798448/1‑140 (MQ=255)                                                                                                                      aCCCGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCATGTgcagca   >  1:823817/1‑140 (MQ=255)                                                                                                                       cccGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCATGTGCAGCAt  <  2:448978/140‑1 (MQ=255)                                                                                                                       cccGCCAGTTCGCGGACATGCAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCATGTGCAGCAt  <  2:839614/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                         GATCTTTTCGGGTCCGATTCGATCGCCAAGTTTGCCGAGTCGTGGTGCACTTAGCAGAGCCGCCACGCCTGGCACCGAGGCGATCATGCCACTGATAAAGGCGACGTTACTGACGTTACCCGCCAGTTCGCGGACATACAGCGTCAGAATGGGGGCAATTGAGCCCGTCGCCACCTGGATGATTAACGTAGTGACAAACAGGCTGAGTACCAGTTTCGGGTTTTTAAGTGATGTCACCACTTCCCGCATGTGCAGCAT  >  NC_000913/1114621‑1114878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑