Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,136,183	A→G	Y207C (TAC→TGC)	flgH →	flagellar L‑ring protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,136,183	0	A	G	100.0%	288.8 / NA	82	Y207C (TAC→TGC)	flgH	flagellar L‑ring protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/38);  total (44/38)

TGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTACTGGTCACGACG  >  NC_000913/1136051‑1136318                                                                                                                                     |                                                                                                                                        tggtggGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGc                                                                                                                                  <  2:1662057/140‑1 (MQ=255)   gtgggtgAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCaa                                                                                                                                >  2:839362/1‑140 (MQ=255)    tgggtgAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGc                                                                                                                                  <  2:1591698/137‑1 (MQ=255)    tgggtgAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGc                                                                                                                                  >  1:1591698/1‑137 (MQ=255)    tgggtgAAAAACAGATCGCCATTAATCAGGGAACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCAGGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAAt                                                                                                                               >  2:1396243/1‑140 (MQ=255)       gtgAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGc                                                                                                                            <  1:86350/140‑1 (MQ=255)       gtgAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGc                                                                                                                            <  1:378338/140‑1 (MQ=255)       gtgAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGc                                                                                                                            <  1:1255497/140‑1 (MQ=255)       gtgAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGc                                                                                                                            <  2:900241/140‑1 (MQ=255)       gtgAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGc                                                                                                                            <  2:79218/140‑1 (MQ=255)       gtgAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGc                                                                                                                            <  1:1597230/140‑1 (MQ=255)       gtgAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGC‑ATGGCt                                                                                                                           >  1:1117783/1‑140 (MQ=255)                  aTTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGa                                                                                                                 >  2:1389169/1‑140 (MQ=255)                    tGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAg                                                                                                               <  2:170649/140‑1 (MQ=255)                        aTTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCa                                                                                                           <  2:915125/140‑1 (MQ=255)                          tAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCaaa                                                                                                         >  2:560808/1‑140 (MQ=255)                                gggTACCGAATTTATTCGCTTCTCTGGCGTGGTTGATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAg                                                                                                               >  2:1469459/1‑128 (MQ=255)                                gggTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAg                                                                                                               <  1:1469459/128‑1 (MQ=255)                                 ggTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATggg                                                                                                  >  2:303160/1‑140 (MQ=255)                                 ggTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATggg                                                                                                  <  1:839362/140‑1 (MQ=255)                                 ggTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACAGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAg                                                                                                               >  1:8054/1‑127 (MQ=255)                                 ggTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACAGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAg                                                                                                               <  2:8054/127‑1 (MQ=255)                                           ttATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAg                                                                                        >  1:524594/1‑140 (MQ=255)                                           ttATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAg                                                                                        >  1:1027258/1‑140 (MQ=255)                                                cGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGttt                                                                                   <  1:560808/140‑1 (MQ=255)                                                cGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGttt                                                                                   <  1:1389169/140‑1 (MQ=255)                                                  cTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTttct                                                                                 >  2:225661/1‑140 (MQ=255)                                                   ttCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGttt                                                                                   >  2:1572149/1‑137 (MQ=255)                                                   ttCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGttt                                                                                   <  1:1572149/137‑1 (MQ=255)                                                    tctcTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTttcttc                                                                               >  2:1016677/1‑140 (MQ=255)                                                             tGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTg                                                                      >  2:908865/1‑140 (MQ=255)                                                                 tAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGc                                                                  <  1:986019/140‑1 (MQ=255)                                                                    tCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCaa                                                               >  1:1641602/1‑140 (MQ=255)                                                                    tCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCaa                                                               >  1:1604419/1‑140 (MQ=255)                                                                    tCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCaa                                                               >  1:930478/1‑140 (MQ=255)                                                                     ccACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAAt                                                              <  2:948969/140‑1 (MQ=255)                                                                         gCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAAt                                                              <  1:711614/136‑1 (MQ=255)                                                                         gCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAAt                                                              >  2:711614/1‑136 (MQ=255)                                                                         gCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTaa                                                          >  1:633463/1‑140 (MQ=255)                                                                         gCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTaa                                                          >  2:1570920/1‑140 (MQ=255)                                                                         gCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTaa                                                          >  1:536266/1‑140 (MQ=255)                                                                         gCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTaa                                                          >  1:147110/1‑140 (MQ=255)                                                                         ccACTATCGGCGGCAGCATTACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGC‑ATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAg                                                         <  2:1117783/139‑1 (MQ=255)                                                                              aTCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAg                                                     >  2:1522455/1‑140 (MQ=255)                                                                               tCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAgg                                                    >  2:412722/1‑140 (MQ=255)                                                                               tCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAgg                                                    >  1:192064/1‑140 (MQ=255)                                                                               tCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAgg                                                    <  2:1578785/140‑1 (MQ=255)                                                                                 aGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCt                                                  <  1:412722/140‑1 (MQ=255)                                                                                  gCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCtg                                                 <  1:1287885/140‑1 (MQ=255)                                                                                    ggCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCtgtg                                               <  2:156819/140‑1 (MQ=255)                                                                                     gcagcaATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCtgtg                                               >  2:382775/1‑139 (MQ=255)                                                                                     gcagcaATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCtgtg                                               <  1:382775/139‑1 (MQ=255)                                                                                     gcagcaATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTgtgg                                              >  2:103312/1‑140 (MQ=255)                                                                                        gcaATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGa                                           <  2:1604419/140‑1 (MQ=255)                                                                                        gcaATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGa                                           <  2:1027258/140‑1 (MQ=255)                                                                                         caATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCtgt                                                <  1:1442223/134‑1 (MQ=255)                                                                                         caATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCtgt                                                >  2:1442223/1‑134 (MQ=255)                                                                                                 taccgtCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTtc                                  >  2:986215/1‑140 (MQ=255)                                                                                                    cgtCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTtctct                               <  1:303160/140‑1 (MQ=255)                                                                                                    cgtCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTtctct                               <  2:1641602/140‑1 (MQ=255)                                                                                                      tCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCTATGTAAGTGAGGCTGTGGTGATTAAATTTctctct                             >  1:336125/1‑140 (MQ=255)                                                                                                      tCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTgtgg                                              <  1:1387093/123‑1 (MQ=255)                                                                                                      tCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTgtgg                                              >  2:1387093/1‑123 (MQ=255)                                                                                                      tCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTtctc                                <  2:80554/137‑1 (MQ=255)                                                                                                      tCTACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTtctc                                >  1:80554/1‑137 (MQ=255)                                                                                                        tACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGc                           >  2:1473333/1‑140 (MQ=255)                                                                                                        tACTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGc                           >  1:1630142/1‑140 (MQ=255)                                                                                                         aCTCAGGTGGCGGATGTGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCa                          >  2:464092/1‑140 (MQ=255)                                                                                                          cTCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCAt                         >  1:1185187/1‑140 (MQ=255)                                                                                                           tCAGGTGGCGGATGTGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCAtt                        <  1:464092/140‑1 (MQ=255)                                                                                                           tCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCAtt                        <  1:1473333/140‑1 (MQ=255)                                                                                                           tCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCAtt                        <  2:524594/140‑1 (MQ=255)                                                                                                           tCAGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCAtt                        <  1:986215/140‑1 (MQ=255)                                                                                                             aGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTaa                      >  1:463477/1‑140 (MQ=255)                                                                                                             aGGTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTaa                      >  1:1377723/1‑140 (MQ=255)                                                                                                              ggTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCAtt                        >  1:879160/1‑137 (MQ=255)                                                                                                              ggTGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCAtt                        <  2:879160/137‑1 (MQ=255)                                                                                                                tGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAAttc                   >  1:791185/1‑140 (MQ=255)                                                                                                                tGGCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAAttc                   >  1:337538/1‑140 (MQ=255)                                                                                                                  gCGGATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAAttctt                 >  2:637961/1‑140 (MQ=255)                                                                                                                     gATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAGGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTa              >  2:472143/1‑140 (MQ=255)                                                                                                                     gATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTa              >  1:1354066/1‑140 (MQ=255)                                                                                                                     gATGCGCGCATTGAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTa              <  2:1354066/140‑1 (MQ=255)                                                                                                                                 gAATGCGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCTATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTACTGGTCacgacg  <  2:336125/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        TGGTGGGTGAAAAACAGATTGCCATTAATCAGGGTACCGAATTTATTCGCTTCTCTGGCGTGGTTAATCCACGCACTATCAGCGGCAGCAATACCGTACCGTCTACTCAGGTGGCGGATGCGCGCATTGAATACGTAGGCAATGGCTACATTAACGAAGCGCAAAATATGGGCTGGTTGCAGCGTTTCTTCCTTAACCTGTCGCCAATGTAAGTGAGGCTGTGGTGATTAAATTTCTCTCTGCATTAATTCTTCTACTGGTCACGACG  >  NC_000913/1136051‑1136318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑