Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,143,140	T→C	T410A (ACT→GCT)	rne ←	ribonuclease E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,143,140	0	T	C	100.0%	256.1 / NA	72	T410A (ACT→GCT)	rne	ribonuclease E
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/35);  total (37/35)

TCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTC  >  NC_000913/1143011‑1143275                                                                                                                                    |                                                                                                                                        tcaGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGaacaa                                                                                                                               >  1:1541710/1‑140 (MQ=255)          aagaagCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACaa                                                                                                                      <  2:1510965/140‑1 (MQ=255)             aagCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACg                                                                                                                   >  2:413218/1‑140 (MQ=255)               gCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGtg                                                                                                                 >  2:856842/1‑140 (MQ=255)               gCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGtg                                                                                                                 >  2:1029200/1‑140 (MQ=255)               gCGATTGGCACAGGAACAATGGCGTGAACTTCCT‑GGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGtga                                                                                                                >  1:1457753/1‑140 (MQ=255)                cGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGtga                                                                                                                <  2:692478/140‑1 (MQ=255)                  aTTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGtgatg                                                                                                              >  1:1169896/1‑140 (MQ=255)                  aTTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGtgatg                                                                                                              >  1:134078/1‑140 (MQ=255)                  aTTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGtgatg                                                                                                              >  2:464980/1‑140 (MQ=255)                  aTTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGtgatg                                                                                                              >  1:376512/1‑140 (MQ=255)                    tGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGAc                                                                                                            <  1:856842/140‑1 (MQ=255)                         caGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGAt                                                                                                       <  2:211932/140‑1 (MQ=255)                         caGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGAt                                                                                                       <  1:73142/140‑1 (MQ=255)                         caGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGAt                                                                                                       <  1:1029200/140‑1 (MQ=255)                         caGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGAt                                                                                                       <  2:1573561/140‑1 (MQ=255)                              aCAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCAcc                                                                                                  <  2:1554586/140‑1 (MQ=255)                              aCAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCAcc                                                                                                  <  2:518666/140‑1 (MQ=255)                               cAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGt                                                                                                                  >  1:304176/1‑123 (MQ=255)                               cAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGt                                                                                                                  <  2:304176/123‑1 (MQ=255)                                aaTGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGAt                                                                                                       <  2:1100407/133‑1 (MQ=255)                                aaTGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGAt                                                                                                       >  1:1100407/1‑133 (MQ=255)                                  tGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAgg                                                                                              >  2:825103/1‑139 (MQ=255)                                    gCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGa                                                                                            <  1:413218/140‑1 (MQ=255)                                     cGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGAt                                                                                           >  2:1267949/1‑140 (MQ=255)                                      gTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGa                                                                                            >  1:417400/1‑140 (MQ=255)                                      gTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATg                                                                                          >  1:695633/1‑140 (MQ=255)                                      gTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATg                                                                                          <  2:71653/140‑1 (MQ=255)                                              ccTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAgg                                                                                  >  1:379701/1‑140 (MQ=255)                                              ccTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAgg                                                                                  >  2:1385093/1‑140 (MQ=255)                                                        ctctTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGAGAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACggg                                                                        >  2:1141250/1‑140 (MQ=255)                                                        ctctTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACggg                                                                        >  1:531869/1‑140 (MQ=255)                                                        ctctTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACggg                                                                        <  2:134078/140‑1 (MQ=255)                                                                 cgcTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGGGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCTCCCAGTGATGGGCTCTGGCGCTGACGGGACAttccag                                                               <  2:1169896/140‑5 (MQ=255)                                                                 cgcTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCca                                                               >  1:42467/1‑140 (MQ=255)                                                                     tcttcttcGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCcagcag                                                           >  2:769611/1‑140 (MQ=255)                                                                       ttcttcGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGc                                                         >  2:428523/1‑140 (MQ=255)                                                                        tcttcGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGcc                                                        <  1:464980/140‑1 (MQ=255)                                                                           tcGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCaaa                                                     <  2:1457753/140‑1 (MQ=255)                                                                              aTCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACTGGACATTTCCAGCAGGCCAAAGCg                                                  >  1:286879/1‑140 (MQ=255)                                                                                   aCGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGaaa                                             >  2:1583617/1‑140 (MQ=255)                                                                                        gAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACTGGACATTTCCAGCAGGCCAAAGCGAGAAATATgg                                        <  2:286879/140‑1 (MQ=255)                                                                                        gAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATgg                                        <  2:858247/140‑1 (MQ=255)                                                                                        gAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATgg                                        <  1:822220/140‑1 (MQ=255)                                                                                        gAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATgg                                        <  1:1141250/140‑1 (MQ=255)                                                                                        gAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATgg                                        <  2:42467/140‑1 (MQ=255)                                                                                         aaT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGc                                       <  1:769611/140‑1 (MQ=255)                                                                                               gagagCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGAt                                   >  2:1243148/1‑140 (MQ=255)                                                                                               gagagCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGAt                                   >  1:1521596/1‑140 (MQ=255)                                                                                                 gagCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGAttt                                 >  2:1337361/1‑140 (MQ=255)                                                                                                 gagCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGAttt                                 >  2:328387/1‑140 (MQ=255)                                                                                                     gACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAata                                           <  2:701666/126‑1 (MQ=255)                                                                                                     gACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAata                                           >  1:701666/1‑126 (MQ=255)                                                                                                     gACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTg                                     <  1:1330437/132‑1 (MQ=255)                                                                                                     gACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTg                                     >  2:1330437/1‑132 (MQ=255)                                                                                                     gACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAAt                             >  2:1380511/1‑140 (MQ=255)                                                                                                            aTTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCa                      <  2:1521596/140‑1 (MQ=255)                                                                                                            aTTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCa                      <  2:695633/140‑1 (MQ=255)                                                                                                            aTTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCa                      <  2:531869/140‑1 (MQ=255)                                                                                                            aTTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCa                      >  1:1470994/1‑140 (MQ=255)                                                                                                            aTTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCa                      >  2:491100/1‑140 (MQ=255)                                                                                                            aTTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCa                      <  1:328387/140‑1 (MQ=255)                                                                                                            aTTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCa                      <  1:428523/140‑1 (MQ=255)                                                                                                               cGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACgg                   <  1:491100/140‑1 (MQ=255)                                                                                                                    tCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATAcgcg                        >  2:349702/1‑130 (MQ=255)                                                                                                                    tCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATAcgcg                        <  1:349702/130‑1 (MQ=255)                                                                                                                    tCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATAcgcg                        >  2:806857/1‑130 (MQ=255)                                                                                                                    tCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATAcgcg                        <  1:806857/130‑1 (MQ=255)                                                                                                                         cacgGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCa         <  1:1337361/140‑1 (MQ=255)                                                                                                                         cacgGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCa         <  1:1267949/140‑1 (MQ=255)                                                                                                                            ggTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCg      <  2:376512/140‑1 (MQ=255)                                                                                                                              tGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCt    >  1:1662043/1‑140 (MQ=255)                                                                                                                                ccAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTc  >  1:953316/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                        TCAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAAT‑‑AGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCACCGCTTC  >  NC_000913/1143011‑1143275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑