Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,366,662	A→G	A371A (GCA→GCG)	puuE →	4‑aminobutyrate aminotransferase PuuE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,366,662	0	A	G	100.0%	319.1 / NA	91	A371A (GCA→GCG)	puuE	4‑aminobutyrate aminotransferase PuuE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/45);  total (46/45)

GGGCCAGCGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCACAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATCCCGGATGCGCAATTCGATGCGGCAATGAAAATTTTG  >  NC_000913/1366527‑1366794                                                                                                                                        |                                                                                                                                     gggCCAGCGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGa                                                                                                                                  <  1:1277316/140‑1 (MQ=255) gggCCAGCGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGa                                                                                                                                  <  2:652114/140‑1 (MQ=255) gggCCAGCGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGa                                                                                                                                  <  1:38388/140‑1 (MQ=255) gggCCAGCGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGa                                                                                                                                  <  1:408981/140‑1 (MQ=255)  ggCCAGCGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGaa                                                                                                                                 >  1:799259/1‑140 (MQ=255)  ggCCAGCGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGaa                                                                                                                                 >  1:596809/1‑140 (MQ=255)        cGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCa                                                                                                                           >  1:1367824/1‑140 (MQ=255)         gTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAGAATCCAg                                                                                                                          <  1:175840/140‑1 (MQ=255)         gTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAg                                                                                                                          >  1:577999/1‑140 (MQ=255)             cAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAAc                                                                                                                      >  2:1355219/1‑140 (MQ=255)              aaaaaCACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAAcg                                                                                                                     <  2:234973/140‑1 (MQ=255)                aaaCACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAAcgcg                                                                                                                   <  2:1195927/140‑1 (MQ=255)                 aaCACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAAcgcgc                                                                                                                  <  1:551542/140‑1 (MQ=255)                      gTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTgg                                                                                                             >  1:1452629/1‑140 (MQ=255)                          attgatGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCa                                                                                                         >  2:1016874/1‑140 (MQ=255)                              atGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAgggg                                                                                                     <  1:1026884/140‑1 (MQ=255)                              atGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAgggg                                                                                                     <  1:820473/140‑1 (MQ=255)                               tGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGgc                                                                                                    <  1:956887/140‑1 (MQ=255)                                       aaagCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCctg                                                                                            <  1:930912/140‑1 (MQ=255)                                          gCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCctgctg                                                                                         <  2:596809/140‑1 (MQ=255)                                          gCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCctgctg                                                                                         <  2:799259/140‑1 (MQ=255)                                           cGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAggg                                                                                                      <  1:651931/126‑1 (MQ=255)                                           cGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAggg                                                                                                      >  2:651931/1‑126 (MQ=255)                                           cGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCctg                                                                                            <  1:160977/136‑1 (MQ=255)                                           cGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCctg                                                                                            >  2:160977/1‑136 (MQ=255)                                            gTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGAc                                                                                       <  1:892213/140‑1 (MQ=255)                                            gTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGAc                                                                                       <  1:1525487/140‑1 (MQ=255)                                             ttCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGAcc                                                                                      >  2:818841/1‑140 (MQ=255)                                             ttCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGAcc                                                                                      >  2:1175611/1‑140 (MQ=255)                                                     aTTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGgcgc                                                                              <  1:385319/140‑1 (MQ=255)                                                       tgctgcGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCAt                                                                            >  2:1042444/1‑140 (MQ=255)                                                            cGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGc                                                                       >  2:1032636/1‑140 (MQ=255)                                                            cGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGc                                                                       >  2:1137148/1‑140 (MQ=255)                                                                aCGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACg                                                                   <  2:1167555/140‑1 (MQ=255)                                                                 cgcgGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGt                                                                  >  1:1360089/1‑140 (MQ=255)                                                                 cgcgGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGt                                                                  >  1:189214/1‑140 (MQ=255)                                                                 cgcgGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGt                                                                  >  2:1157450/1‑140 (MQ=255)                                                                 cgcgGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGt                                                                  >  1:797020/1‑140 (MQ=255)                                                                   cgGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGa                                                                >  2:164443/1‑140 (MQ=255)                                                                   cgGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGa                                                                >  1:696848/1‑140 (MQ=255)                                                                   cgGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGa                                                                <  1:681913/140‑1 (MQ=255)                                                                   cgGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGa                                                                <  1:143685/140‑1 (MQ=255)                                                                    ggCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGAt                                                               <  1:1016874/140‑1 (MQ=255)                                                                     gCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGAtt                                                              <  1:548011/140‑1 (MQ=255)                                                                      ccTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTc                                                             >  2:1381447/1‑140 (MQ=255)                                                                      ccTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTc                                                             >  2:46749/1‑140 (MQ=255)                                                                      ccTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTc                                                             >  2:1419993/1‑140 (MQ=255)                                                                       cTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCg                                                            <  1:252158/140‑1 (MQ=255)                                                                       cTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCg                                                            <  2:76780/140‑1 (MQ=255)                                                                        tGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGc                                                           <  1:1042444/140‑1 (MQ=255)                                                                           ggTCGATGATTGCGGTAGAGTTTAACGATCAGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTc                                                        >  1:841411/1‑140 (MQ=255)                                                                               gatgatTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGt                                                    >  2:787361/1‑140 (MQ=255)                                                                               gatgatTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGt                                                    <  2:1367824/140‑1 (MQ=255)                                                                                atgatTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGt                                                                  >  1:1527137/1‑125 (MQ=255)                                                                                atgatTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGt                                                                  <  2:1527137/125‑1 (MQ=255)                                                                                atgatTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCg                                                            <  1:607134/131‑1 (MQ=255)                                                                                atgatTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCg                                                            >  2:607134/1‑131 (MQ=255)                                                                                atgatTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTa                                                   >  1:1489127/1‑140 (MQ=255)                                                                                atgatTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTa                                                   >  2:1520688/1‑140 (MQ=255)                                                                                atgatTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTa                                                   >  2:1625451/1‑140 (MQ=255)                                                                                     tGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGc                                              <  1:164443/140‑1 (MQ=255)                                                                                      gCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCt                                             >  1:453073/1‑140 (MQ=255)                                                                                       cGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTg                                            >  2:1556873/1‑140 (MQ=255)                                                                                       cGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATACAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTg                                            <  2:1452629/140‑1 (MQ=255)                                                                                             agTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATc                                      <  1:1588009/140‑1 (MQ=255)                                                                                             agTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATc                                      <  2:821253/140‑1 (MQ=255)                                                                                               tttAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATccc                                    >  2:360984/1‑140 (MQ=255)                                                                                               tttAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATccc                                    >  1:1094537/1‑140 (MQ=255)                                                                                                 tAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATCCCgg                                  <  1:1661988/140‑1 (MQ=255)                                                                                                 tAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATCCCgg                                  <  2:210806/140‑1 (MQ=255)                                                                                                 tAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATCCCgg                                  <  2:310828/140‑1 (MQ=255)                                                                                                 tAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATCCCgg                                  >  2:880936/1‑140 (MQ=255)                                                                                                      aTCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATCCCGGATgcg                             <  2:841411/140‑1 (MQ=255)                                                                                                       tCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATCCCGGATgcgc                            >  2:1532441/1‑140 (MQ=255)                                                                                                        ccGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATCCCGGATGCGCa                           >  2:260943/1‑140 (MQ=255)                                                                                                         cGCAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATCCCGGATGCGCaa                          >  1:781203/1‑140 (MQ=255)                                                                                                           cAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCAt                                       <  2:1307692/125‑1 (MQ=255)                                                                                                           cAAACGGGCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCAt                                       >  1:1307692/1‑125 (MQ=255)                                                                                                                gggCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATCCCGGATGCGCAATTCGATg                   <  1:1532441/140‑1 (MQ=255)                                                                                                                gggCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATCCCGGATGCGCAATTCGATg                   <  2:797020/140‑1 (MQ=255)                                                                                                                 ggCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATCCCGGATGCGCAATTCGATGc                  >  1:274458/1‑140 (MQ=255)                                                                                                                 ggCGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATCCCGGATGCGCAATTCGATGc                  >  1:638712/1‑140 (MQ=255)                                                                                                                   cGAGCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATCCCGGATGCGCAATTCGATGCgg                >  1:429607/1‑140 (MQ=255)                                                                                                                     aGCCATCAGCGGCGCTTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGAGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATCCCGGATGCGCAATTCGATGCGGCa              >  2:531690/1‑140 (MQ=255)                                                                                                                      gCCGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATCCCGGATGCGCAATTCGATGCGGCaa             >  1:494967/1‑140 (MQ=255)                                                                                                                       ccGTCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATCCCGGATGCGCAATTCGATGCGGCAAt            <  1:1175611/140‑1 (MQ=255)                                                                                                                          tCAGCGGCGATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATCCCGGATGCGCAATTCGATGCGGCAATGaa         >  1:1361231/1‑140 (MQ=255)                                                                                                                              cggcgATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATCCCGGATGCGCAATTCGATGCGGCAATGAAAAtt     <  2:189214/140‑1 (MQ=255)                                                                                                                                gcgATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGTGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATCCCGGATGCGCAATTCGATGCGGCAATGAAAAtttt   <  1:531690/140‑1 (MQ=255)                                                                                                                                gcgATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATCCCGGATGCGCAATTCGATGCGGCAATGAAAAtttt   <  2:1094537/140‑1 (MQ=255)                                                                                                                                 cgATTGCGCAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATCCCGGATGCGCAATTCGATGCGGCAATGAAAATTTTg  >  1:125523/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     GGGCCAGCGTCTCAAAAACACGTTGATTGATGCCAAAGAAAGCGTTCCGGCCATTGCTGCGGTACGCGGCCTGGGGTCGATGATTGCGGTAGAGTTTAACGATCCGCAAACGGGCGAGCCGTCAGCGGCGATTGCACAGAAAATCCAGCAACGCGCGCTGGCGCAGGGGCTGCTCCTGCTGACCTGTGGCGCATACGGCAACGTGATTCGCTTCCTGTATCCGCTGACCATCCCGGATGCGCAATTCGATGCGGCAATGAAAATTTTG  >  NC_000913/1366527‑1366794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑