Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,493,424	C→T	Q319* (CAA→TAA)	trg →	methyl‑accepting chemotaxis protein Trg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,493,424	0	C	T	100.0%	269.3 / NA	80	Q319* (CAA→TAA)	trg	methyl‑accepting chemotaxis protein Trg
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/41);  total (39/41)

TGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGCAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGACGGTTTCCGGTGTAGTAAAAACGATGGGCGCTATCTCCA  >  NC_000913/1493287‑1493559                                                                                                                                          |                                                                                                                                        tGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTaa                                                                                                                                       >  2:644802/1‑140 (MQ=255)     aGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCa                                                                                                                                   >  2:1079354/1‑140 (MQ=255)       tAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACt                                                                                                                                 >  1:831987/1‑140 (MQ=255)         gggACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGc                                                                                                                               >  1:959566/1‑140 (MQ=255)            aCTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGAc                                                                                                                            >  1:729595/1‑140 (MQ=255)             cTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACg                                                                                                                           <  2:748349/140‑1 (MQ=255)              tGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACgg                                                                                                                          >  1:1322685/1‑140 (MQ=255)              tGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACgg                                                                                                                          >  2:5554/1‑140 (MQ=255)                 tCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGa                                                                                                                       >  1:1522160/1‑140 (MQ=255)                   gACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGaaa                                                                                                                     <  2:959566/140‑1 (MQ=255)                   gACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGaaa                                                                                                                     >  1:444799/1‑140 (MQ=255)                    aCAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAAc                                                                                                                    <  1:1412002/140‑1 (MQ=255)                       gggCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGa                                                                                                                 >  2:1298034/1‑140 (MQ=255)                          cgcgGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAAc                                                                                                                    <  2:1203137/134‑1 (MQ=255)                          cgcgGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAAc                                                                                                                    >  1:1203137/1‑134 (MQ=255)                          cgcgGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATg                                                                                                              >  1:1364873/1‑140 (MQ=255)                          cgcgGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATg                                                                                                              >  2:623658/1‑140 (MQ=255)                          cgcgGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATg                                                                                                              >  2:1569399/1‑140 (MQ=255)                          cgcgGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATg                                                                                                              >  1:757681/1‑140 (MQ=255)                               aaGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGAt                                                                                                         <  1:760524/140‑1 (MQ=255)                                 gagaTTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATaa                                                                                                       <  2:757681/140‑1 (MQ=255)                                 gagaTTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATaa                                                                                                       <  1:1622512/140‑1 (MQ=255)                                  agaTTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAAc                                                                                                      >  2:566923/1‑140 (MQ=255)                                  agaTTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAAc                                                                                                      >  2:1640439/1‑140 (MQ=255)                                   gaTTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAAcg                                                                                                     <  1:1030234/140‑1 (MQ=255)                                   gaTTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAAcg                                                                                                     <  2:703180/140‑1 (MQ=255)                                   gaTTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAAcg                                                                                                     <  1:1364935/140‑1 (MQ=255)                                      ttATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACgcgc                                                                                                  <  2:729595/140‑1 (MQ=255)                                         tCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGcatc                                                                                               >  1:1511170/1‑140 (MQ=255)                                              gCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGcc                                                                                          >  1:1353498/1‑140 (MQ=255)                                              gCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGcc                                                                                          >  1:947940/1‑140 (MQ=255)                                                   aGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGcat                                                                                                >  2:1172589/1‑129 (MQ=255)                                                   aGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGcat                                                                                                <  1:1172589/129‑1 (MQ=255)                                                   aGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCaa                                                                                     >  2:18079/1‑140 (MQ=255)                                                        aaTTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTgg                                                                                <  2:444799/140‑1 (MQ=255)                                                           ttCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGC‑AACTGGCGCa                                                                            <  2:1353498/140‑1 (MQ=255)                                                             cAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCaa                                                                           <  1:18079/140‑1 (MQ=255)                                                              aGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAag                                                                          <  1:1650233/140‑1 (MQ=255)                                                               gCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTgg                                                                                <  1:617856/133‑1 (MQ=255)                                                               gCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTgg                                                                                >  2:617856/1‑133 (MQ=255)                                                               gCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCaa                                                                           <  1:1378767/138‑1 (MQ=255)                                                               gCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCaa                                                                           >  2:1378767/1‑138 (MQ=255)                                                               gCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAaga                                                                         <  1:5554/140‑1 (MQ=255)                                                                  ggCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGc                                                                      <  2:1511170/140‑1 (MQ=255)                                                                  ggCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGc                                                                      <  1:623658/140‑1 (MQ=255)                                                                  ggCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCACGCCAGCAAACTGGCGCAAGAGGc                                                                      <  2:1522160/140‑1 (MQ=255)                                                                    cAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCtt                                                                    <  2:533172/140‑1 (MQ=255)                                                                    cAATGCGGACCTGTCATCTCGCACCAAAGAACAGGCGGCGGCTATCGACCAACCTGCCGGTAGCATGGAGTAACCCACTGCGAAGGTGAAACAGAATGCGGATAACGAGCATCATGCCAGCAAACTGGCGCAAGAGGAtt                                                                    <  2:251144/140‑1 (MQ=255)                                                                         cGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTAtt                                                               <  1:1386054/140‑1 (MQ=255)                                                                             ccTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAg                                                           >  2:1434844/1‑140 (MQ=255)                                                                             ccTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAg                                                           >  1:1356815/1‑140 (MQ=255)                                                                             ccTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAg                                                           >  2:1338776/1‑140 (MQ=255)                                                                                 tCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAg                                                       >  1:389203/1‑140 (MQ=255)                                                                                        gCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGc                                                >  1:1432857/1‑140 (MQ=255)                                                                                           ccGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCggg                                             >  1:387837/1‑140 (MQ=255)                                                                                           ccGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCggg                                             >  1:494540/1‑140 (MQ=255)                                                                                             gaagaaCAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCa                                           >  2:40757/1‑140 (MQ=255)                                                                                              aagaaCAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAg                                          >  2:563497/1‑140 (MQ=255)                                                                                               agaaCAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGa                                         <  1:59558/140‑1 (MQ=255)                                                                                               agaaCAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGa                                         <  1:1569399/140‑1 (MQ=255)                                                                                               agaaCAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGa                                         <  2:947940/140‑1 (MQ=255)                                                                                                      gcggcgGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGACGGTTTc                                  <  2:791949/140‑1 (MQ=255)                                                                                                      gcggcgGCTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGACGGTTTc                                  <  2:1101007/140‑1 (MQ=255)                                                                                                        ggcggcTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGACGGTTCCCg                                >  2:1144511/1‑140 (MQ=255)                                                                                                           ggcTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGACGGTTTCCGgtg                             >  2:739616/1‑140 (MQ=255)                                                                                                            gcTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGACGGTTTCCGgtgt                            >  2:174944/1‑140 (MQ=255)                                                                                                            gcTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGACGGTTTCCGgtgt                            <  1:1298034/140‑1 (MQ=255)                                                                                                            gcTATCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGACGGTTCCCGgtgt                            <  1:1144511/140‑1 (MQ=255)                                                                                                                tCGAACAAACTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGACGGTTTCCGGTgtagta                        >  1:1446960/1‑140 (MQ=255)                                                                                                                        aCTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGACGGTTTCCGGTGTAGTAAAAACGAt                <  2:1356815/140‑1 (MQ=255)                                                                                                                        aCTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGACGGTTTCCGGTGTAGTAAAAACGAt                <  2:1136698/140‑1 (MQ=255)                                                                                                                        aCTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGACGGTTTCCGGTGTAGTAAAAACGAt                <  1:739616/140‑1 (MQ=255)                                                                                                                        aCTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGACGGTTTCCGGTGTAGTAAAAACGAt                <  1:566923/140‑1 (MQ=255)                                                                                                                        aCTGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGACGG‑TTCCGGTGTAGTAAAAACGATg               >  2:726516/1‑140 (MQ=255)                                                                                                                          tGCCGCCAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGACGGTTTCCGGTGTAGTAAAAACGATgg              >  2:817541/1‑140 (MQ=255)                                                                                                                              gccAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGACGG‑TTCCGGTGTAGTAAAAACGATGGGCGCt         <  1:726516/140‑1 (MQ=255)                                                                                                                               ccAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGACGGTTTCCGGTGTAGTAAAAACGATGGGCGCt         <  2:387837/140‑1 (MQ=255)                                                                                                                               ccAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGACGGTTTCCGGTGTAGTAAAAACGATGGGCGCt         <  2:1364873/140‑1 (MQ=255)                                                                                                                               ccAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGACGGTTTCCGGTGTAGTAAAAACGATGGGCGCt         <  2:1432857/140‑1 (MQ=255)                                                                                                                               ccAGCATGGAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGACGGTTTCCGGTGTAGTAAAAACGATGGGCGCt         <  1:1079354/140‑1 (MQ=255)                                                                                                                                      ggAGTAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGACGGTTTCCGGTGTAGTAAAAACGATGGGCGCTATCTCCa  <  2:1322685/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TGACAGTAGGGACTGTTCGACAGGGCGCGGAAGAGATTTATCGTGGCACCAGCGAAATTTCAGCTGGCAATGCGGACCTGTCATCTCGCACCGAAGAACAAGCGGCGGCTATCGAACAAACTGCCGCCAGCATGGAGCAACTCACTGCGACGGTGAAACAGAATGCGGATAACGCGCATCATGCCAGCAAACTGGCGCAAGAGGCTTCTATTAAAGCCAGCGATGGCGGGCAGACGGTTTCCGGTGTAGTAAAAACGATGGGCGCTATCTCCA  >  NC_000913/1493287‑1493559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑