Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,513,875	T→C	G15G (GGT→GGC)	ydcU →	putative ABC transporter membrane subunit YdcU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,513,875	0	T	C	100.0%	230.9 / NA	65	G15G (GGT→GGC)	ydcU	putative ABC transporter membrane subunit YdcU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/33);  total (32/33)

GCGAAGAACTGCCTGCCACGCTCACGCCCGGACAACAGGTGATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACG  >  NC_000913/1513739‑1514011                                                                                                                                         |                                                                                                                                          gCGAAGAACTGCCTGCCACGCTCACGCCCGGACAACAGGTGATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAg                                                                                                                                        >  2:76958/1‑140 (MQ=255)                 cACGCTCACGCCCGGACAACAGGTGATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTtcttct                                                                                                                        >  2:237992/1‑140 (MQ=255)                   cGCTCACGCCCGGACAACAGGTGATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTgg                                                                                                                      >  2:492980/1‑140 (MQ=255)                          gCCCGGACAACAGGTGATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATc                                                                                                               >  1:1517119/1‑140 (MQ=255)                               gACAACAGGTGATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCa                                                                                                                    <  2:1647587/130‑1 (MQ=255)                               gACAACAGGTGATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCa                                                                                                                    >  1:1647587/1‑130 (MQ=255)                               gACAACAGGTGATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGAGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑gggg                                                                                                         >  2:1148527/1‑140 (MQ=255)                                acaacaGGTGATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑ggggc                                                                                                        >  2:1283601/1‑140 (MQ=255)                                    caGGTGATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑ggggctggg                                                                                                    <  1:164682/140‑1 (MQ=255)                                     aGGTGATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑ggggctgggg                                                                                                   <  2:1310790/140‑1 (MQ=255)                                     aGGTGATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑ggggctgggg                                                                                                   <  1:76958/140‑1 (MQ=255)                                      ggTGATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑ggggctggggc                                                                                                  <  2:1517119/140‑1 (MQ=255)                                       gTGATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑ggggctggggct                                                                                                 <  1:66182/140‑1 (MQ=255)                                         gATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCGATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGt                                                                                               >  2:1155280/1‑140 (MQ=255)                                          aTGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGtt                                                                                              >  1:1167800/1‑140 (MQ=255)                                          aTGGTTTCCTGGACGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGCCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGtt                                                                                              >  1:160871/1‑140 (MQ=255)                                            ggTTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGtttt                                                                                            >  2:986488/1‑140 (MQ=255)                                                tCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTAct                                                                                        <  2:1273789/140‑1 (MQ=255)                                                  cTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑ggggctggggct                                                                                                 >  2:170569/1‑129 (MQ=255)                                                  cTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑ggggctggggct                                                                                                 <  1:170569/129‑1 (MQ=255)                                                       cgcgTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCtt                                                                                 >  1:1276037/1‑140 (MQ=255)                                                        gcgTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCCAGGGCCTGGGGCGGTTTTAACTGTTGCtt                                                                                 >  1:476602/1‑140 (MQ=255)                                                         cgTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTgg                                                                               >  1:1422920/1‑140 (MQ=255)                                                         cgTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTgg                                                                               <  1:1198028/140‑1 (MQ=255)                                                         cgTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGACTGTTTTTACTGCTGCTTgg                                                                               <  1:1283601/140‑1 (MQ=255)                                                           tgatgtgatgGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGcc                                                                             >  1:250140/1‑140 (MQ=255)                                                           tgatgtgatgGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGcc                                                                             <  2:1167800/140‑1 (MQ=255)                                                                  atgGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAAt                                                                      <  2:1276037/140‑1 (MQ=255)                                                                  atgGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAAt                                                                      >  1:1148827/1‑140 (MQ=255)                                                                   tgGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAAtg                                                                     <  2:1422920/140‑1 (MQ=255)                                                                           cTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTgg                                                             <  2:958520/140‑1 (MQ=255)                                                                                      gagGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTAtt                                                  <  1:1148527/140‑1 (MQ=255)                                                                                        ggTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTc                                                >  1:1126848/1‑140 (MQ=255)                                                                                        ggTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTc                                                >  2:1248724/1‑140 (MQ=255)                                                                                        ggTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTc                                                >  1:378734/1‑140 (MQ=255)                                                                                         gTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCg                                               >  1:163767/1‑140 (MQ=255)                                                                                            aaTGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCt                                            <  2:1148827/140‑1 (MQ=255)                                                                                             aTGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTc                                           <  1:237992/140‑1 (MQ=255)                                                                                              tGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCg                                          >  2:591061/1‑140 (MQ=255)                                                                                               ggCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCgc                                         <  2:250140/140‑1 (MQ=255)                                                                                               ggCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCg                                          >  2:816413/1‑139 (MQ=255)                                                                                               ggCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCg                                          <  1:816413/139‑1 (MQ=255)                                                                                                  gATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCgctgc                                      <  2:796969/140‑1 (MQ=255)                                                                                                   aTGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCgctgct                                     >  1:417079/1‑140 (MQ=255)                                                                                                    tGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCCGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCgc                                         <  2:1383034/135‑1 (MQ=255)                                                                                                    tGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCCGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCgc                                         >  1:1383034/1‑135 (MQ=255)                                                                                                     gAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGa                                   <  2:190904/140‑1 (MQ=255)                                                                                                     gAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGa                                   <  2:417079/140‑1 (MQ=255)                                                                                                     gAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGa                                   <  2:1126848/140‑1 (MQ=255)                                                                                                        tGTATTGCAATCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGacac                                <  1:1260533/140‑1 (MQ=255)                                                                                                                 aTCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGacac                                >  1:1578492/1‑131 (MQ=255)                                                                                                                 aTCACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGacac                                <  2:1578492/131‑1 (MQ=255)                                                                                                                   cACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAg                     <  1:986488/140‑1 (MQ=255)                                                                                                                   cACCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAg                     <  2:38241/140‑1 (MQ=255)                                                                                                                    aCCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAgg                    <  1:1155280/140‑1 (MQ=255)                                                                                                                    aCCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTG‑TTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAg                     >  2:335453/1‑138 (MQ=255)                                                                                                                    aCCTTCACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTG‑TTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAg                     <  1:335453/138‑1 (MQ=255)                                                                                                                         cACGTCCAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAttt               >  2:809043/1‑140 (MQ=255)                                                                                                                              ccAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTtata           >  2:419473/1‑139 (MQ=255)                                                                                                                              ccAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTtata           <  1:419473/139‑1 (MQ=255)                                                                                                                              ccAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTtata           <  1:217774/139‑1 (MQ=255)                                                                                                                              ccAGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTtata           >  2:217774/1‑139 (MQ=255)                                                                                                                                aGGTCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACtt        >  1:970651/1‑140 (MQ=255)                                                                                                                                   tCTGGGCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATAATTTTg     >  1:5565/1‑140 (MQ=255)                                                                                                                                      gggCAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACg  <  2:378734/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                          GCGAAGAACTGCCTGCCACGCTCACGCCCGGACAACAGGTGATGGTTTCCTGGTCGCGTGATGTGATGGTGCCGCTGGTTGAGGAGAGGTGAATGGCGATGAATGTATTGCAATCACCTTCACGTCCAGGTCTGGGTAAGGTGTCCGGTTTCTTCTGGCATAATCC‑GGGGCTGGGGCTGTTTTTACTGCTGCTTGGCCCGCTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACG  >  NC_000913/1513739‑1514011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑