Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,541,351	C→T	E414K (GAA→AAA)	narZ ←	nitrate reductase Z subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,541,351	0	C	T	100.0%	215.8 / NA	61	E414K (GAA→AAA)	narZ	nitrate reductase Z subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/29);  total (32/29)

TTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTCGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCTT  >  NC_000913/1541217‑1541487                                                                                                                                       |                                                                                                                                         tttAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCC‑AAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTc                                                                                                                                    >  2:1350990/1‑140 (MQ=255)   tAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTcc                                                                                                                                   >  1:1262223/1‑140 (MQ=255)        ggCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTAcc                                                                                                                              >  2:22260/1‑140 (MQ=255)        ggCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTAcc                                                                                                                              >  2:1084458/1‑140 (MQ=255)        ggCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTAcc                                                                                                                              <  1:929761/140‑1 (MQ=255)        ggCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATCTGGTTTCCGTAcc                                                                                                                              <  2:570810/140‑1 (MQ=255)          cAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACcgg                                                                                                                            <  2:1262223/140‑1 (MQ=255)             ttGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACcggcgg                                                                                                                         <  1:337899/140‑1 (MQ=255)               ggCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCa                                                                                                                       <  2:1371470/140‑1 (MQ=255)                 cgcACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAAt                                                                                                                     <  2:795644/140‑1 (MQ=255)                 cgcACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAAt                                                                                                                     <  1:1544327/140‑1 (MQ=255)                  gcACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATg                                                                                                                    <  2:181817/140‑1 (MQ=255)                   cACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATgg                                                                                                                   <  2:1394370/140‑1 (MQ=255)                   cACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATgg                                                                                                                   <  1:624731/140‑1 (MQ=255)                    aCCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGga                                                                                                                  >  2:1101436/1‑140 (MQ=255)                      cAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGgatt                                                                                                                <  1:194253/140‑1 (MQ=255)                           ccGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGgattccaga                                                                                                           >  2:754939/1‑140 (MQ=255)                                   tgtTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCAttt                                                                                                   >  1:922259/1‑140 (MQ=255)                                      tttACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGcc                                                                                                <  2:146677/140‑1 (MQ=255)                                             tGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTTACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTtct                                                                                         >  2:684180/1‑140 (MQ=255)                                             tGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACTACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTtct                                                                                         >  2:171485/1‑140 (MQ=255)                                             tGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTtct                                                                                         >  2:396334/1‑140 (MQ=255)                                             tGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTtct                                                                                         >  1:1634755/1‑140 (MQ=255)                                             tGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTtct                                                                                         >  1:792869/1‑140 (MQ=255)                                               cGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTcc                                                                                       >  1:352392/1‑140 (MQ=255)                                                   aaTGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAg                                                                                   <  1:754939/140‑1 (MQ=255)                                                          aTTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAAc                                                                            <  1:396334/140‑1 (MQ=255)                                                             ttCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCaa                                                                         <  1:1084458/140‑1 (MQ=255)                                                                aaTGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCg                                                                      <  1:633839/140‑1 (MQ=255)                                                                  tGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACAGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGaa                                                                    <  1:1274516/140‑1 (MQ=255)                                                                    ccgccAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAAcc                                                                  >  2:122971/1‑140 (MQ=255)                                                                              aGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACt                                                        <  2:792869/140‑1 (MQ=255)                                                                                gggAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACTACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTAc                                                      <  1:171485/140‑1 (MQ=255)                                                                                 ggAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGt                                                                <  1:1310963/129‑1 (MQ=255)                                                                                 ggAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGt                                                                >  2:1310963/1‑129 (MQ=255)                                                                                    aGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAAt                                                  >  2:903137/1‑140 (MQ=255)                                                                                      gCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCAc                                                         >  1:657456/1‑131 (MQ=255)                                                                                      gCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCAc                                                         <  2:657456/131‑1 (MQ=255)                                                                                      gCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTc                                                >  1:1424984/1‑140 (MQ=255)                                                                                      gCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTc                                                >  1:1602645/1‑140 (MQ=255)                                                                                       ccACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACt                                                        <  2:569663/131‑1 (MQ=255)                                                                                       ccACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACt                                                        >  1:569663/1‑131 (MQ=255)                                                                                        cACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCAc                                              <  1:1605464/140‑1 (MQ=255)                                                                                          cGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCt                                            <  1:1101436/140‑1 (MQ=255)                                                                                           gCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTg                                           <  1:903137/140‑1 (MQ=255)                                                                                                cAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTa                                      >  1:1521090/1‑140 (MQ=255)                                                                                                      cGTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACt                                >  1:605271/1‑140 (MQ=255)                                                                                                       gTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTTTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTg                               >  1:235895/1‑140 (MQ=255)                                                                                                       gTCATGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTg                               >  2:7579/1‑140 (MQ=255)                                                                                                           tGTTGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTAc                           >  2:858914/1‑140 (MQ=255)                                                                                                             ttGACCGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACgg                         >  2:175479/1‑140 (MQ=255)                                                                                                                  cGAGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTc                    <  2:605271/140‑1 (MQ=255)                                                                                                                    aGCAGGGTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCa                  >  2:654412/1‑140 (MQ=255)                                                                                                                         ggTTAACGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCg             <  1:654412/140‑1 (MQ=255)                                                                                                                              aCGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGAttg        >  2:193541/1‑140 (MQ=255)                                                                                                                              aCGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGAttg        >  2:631547/1‑140 (MQ=255)                                                                                                                              aCGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGAttg        >  1:359943/1‑140 (MQ=255)                                                                                                                               cGACAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGAttgt       >  2:56912/1‑140 (MQ=255)                                                                                                                                 aCAATTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGAttgttg     >  2:337989/1‑140 (MQ=255)                                                                                                                                    aTTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCtt  <  2:1448489/140‑1 (MQ=255)                                                                                                                                    aTTTGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCtt  <  1:684180/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         TTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACCGAGCAGGGTTAACGACAATTCGGTTTCCGTACCGGCGGCAATGGATTCCAGATTCCATTTGCCTTTTTCTCCCCAGCGGAAACCAATCGAACCGTTCGGCACTACCAATTCACCTGCGGTATTAACTGCTACGGTTTTCCACTGCGGATTGTTGCTT  >  NC_000913/1541217‑1541487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑