Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,585,936	T→C	E184G (GAA→GGA)	ydeP ←	putative oxidoreductase YdeP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,585,936	0	T	C	100.0%	309.6 / NA	87	E184G (GAA→GGA)	ydeP	putative oxidoreductase YdeP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (46/41);  total (46/41)

GTCAGCATGCGAGGGTGGTTTGTACCAGGGTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAACCTGATTGGGATCACTAT  >  NC_000913/1585804‑1586065                                                                                                                                     |                                                                                                                                  gTCAGCATGCGAGGGTGGTTTGTACCAGGGTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGca                                                                                                                            <  2:821013/140‑1 (MQ=255) gTCAGCATGCGAGGGTGGTTTGTACCAGGGTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGca                                                                                                                            <  1:1127463/140‑1 (MQ=255)   cAGCATGCGAGGGTGGTTTGTACCAGGGTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGcaca                                                                                                                          <  1:1020696/140‑1 (MQ=255)     gCATGCGAGGGTGGTTTGTACCAGGGTTACGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATg                                                                                                                        >  1:704602/1‑140 (MQ=255)      cATGCGAGGGTGGTTTGTACCAGGGTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGca                                                                                                                            <  1:1061462/135‑1 (MQ=255)      cATGCGAGGGTGGTTTGTACCAGGGTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGca                                                                                                                            >  2:1061462/1‑135 (MQ=255)           gAGGGTGGTTTGTACCAGGGTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAg                                                                                                                  <  1:1109657/140‑1 (MQ=255)            aGGGTGGTTTGTACCAGGGTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGc                                                                                                                 <  1:443912/140‑1 (MQ=255)            aGGGTGGTTTGTACCAGGGTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGc                                                                                                                 >  1:884916/1‑140 (MQ=255)                  gtTTGTACCAGGGTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCgg                                                                                                           <  1:1426587/140‑1 (MQ=255)                  gtTTGTACCAGGGTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCgg                                                                                                           >  2:764308/1‑140 (MQ=255)                  gtTTGTACCAGGGTTACGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCgg                                                                                                           <  2:704602/140‑1 (MQ=255)                            gggTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTAtt                                                                                                 >  2:1500866/1‑140 (MQ=255)                            gggTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTAtt                                                                                                 >  2:528140/1‑140 (MQ=255)                             ggTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACGCGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTg                                                                                                <  1:1496983/140‑1 (MQ=255)                               ttATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCt                                                                                              >  1:879853/1‑140 (MQ=255)                                tATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTc                                                                                             <  1:698252/140‑1 (MQ=255)                                 aTGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTcc                                                                                            >  1:1340062/1‑140 (MQ=255)                                 aTGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTcc                                                                                            >  2:403392/1‑140 (MQ=255)                                 aTGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTCGGAGCAGTCGGGAAAGTTATTGCTcc                                                                                            >  2:1048528/1‑140 (MQ=255)                                  tGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTccc                                                                                           >  1:253570/1‑140 (MQ=255)                                  tGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTccc                                                                                           >  1:1324558/1‑140 (MQ=255)                                            aaaTGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACgg                                                                                 >  2:1410979/1‑140 (MQ=255)                                              aTGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGc                                                                               >  2:55009/1‑140 (MQ=255)                                                gACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCaa                                                                             <  1:744600/140‑1 (MQ=255)                                                    aaaTCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAg                                                                         <  1:918919/140‑1 (MQ=255)                                                     aaTCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGc                                                                        >  1:821861/1‑140 (MQ=255)                                                          cACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATa                                                                   >  2:750462/1‑140 (MQ=255)                                                               ctcAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATaaagaa                                                              >  1:1144638/1‑140 (MQ=255)                                                                    aGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCa                                                         <  1:403392/140‑1 (MQ=255)                                                                      tCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGc                                                       <  2:181261/140‑1 (MQ=255)                                                                      tCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGc                                                       <  1:700215/140‑1 (MQ=255)                                                                       cTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATaaagaaa                                                             <  1:1372547/133‑1 (MQ=255)                                                                       cTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATaaagaaa                                                             >  2:1372547/1‑133 (MQ=255)                                                                       cTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCa                                                         >  1:1540874/1‑137 (MQ=255)                                                                       cTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCa                                                         <  2:1540874/137‑1 (MQ=255)                                                                         tCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTc                                                    >  2:1116918/1‑140 (MQ=255)                                                                          ccAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCa                                                   >  1:86844/1‑140 (MQ=255)                                                                                acacGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCTGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGaa                                             >  1:86979/1‑140 (MQ=255)                                                                                  acGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGt                                           >  2:488535/1‑140 (MQ=255)                                                                                     gTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCg                                        <  2:253570/140‑1 (MQ=255)                                                                                      tCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCgg                                       >  1:954652/1‑140 (MQ=255)                                                                                       cccTTTACCTACACCGATCCTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGc                                      <  2:1221736/140‑1 (MQ=255)                                                                                       cccTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGc                                      <  1:1116918/140‑1 (MQ=255)                                                                                       cccTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGc                                      <  1:1410979/140‑1 (MQ=255)                                                                                       cccTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGc                                      <  1:619730/140‑1 (MQ=255)                                                                                         cTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGccc                                    >  2:1634237/1‑140 (MQ=255)                                                                                          tttACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCg                                   <  2:884916/140‑1 (MQ=255)                                                                                          tttACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCg                                   <  2:821861/140‑1 (MQ=255)                                                                                           ttACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGc                                      <  1:1232939/136‑1 (MQ=255)                                                                                           ttACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGc                                      >  2:1232939/1‑136 (MQ=255)                                                                                           ttACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGa                                  >  1:596417/1‑140 (MQ=255)                                                                                               cTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGta                              <  1:750462/140‑1 (MQ=255)                                                                                                tACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGtat                             <  2:596417/140‑1 (MQ=255)                                                                                                tACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGtat                             <  1:764308/140‑1 (MQ=255)                                                                                                tACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGtat                             >  2:1455662/1‑140 (MQ=255)                                                                                                tACACCGATACTCGCTGCCAAACCCACGCTTGTCGCTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAATGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGtat                             <  2:1144638/140‑1 (MQ=255)                                                                                                  cacCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAg                           >  2:503450/1‑140 (MQ=255)                                                                                                  cacCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAg                           >  1:42712/1‑140 (MQ=255)                                                                                                   acCGATACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGa                          >  1:725458/1‑140 (MQ=255)                                                                                                       aTACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCGCATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGATAGGCAGCGTCATTGGAAGTGCGGCCCGAAGTATAGAATTc                      >  2:1467433/1‑140 (MQ=255)                                                                                                       aTACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCg                                   <  1:640387/127‑1 (MQ=255)                                                                                                       aTACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCg                                   >  2:640387/1‑127 (MQ=255)                                                                                                       aTACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGaa                                 <  1:409241/129‑1 (MQ=255)                                                                                                       aTACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGaa                                 >  2:409241/1‑129 (MQ=255)                                                                                                       aTACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAAtt                       <  1:432738/139‑1 (MQ=255)                                                                                                       aTACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAAtt                       >  2:432738/1‑139 (MQ=255)                                                                                                        tACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCa                     >  2:236178/1‑140 (MQ=255)                                                                                                        tACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCa                     >  2:314699/1‑140 (MQ=255)                                                                                                        tACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCa                     <  1:1256763/140‑1 (MQ=255)                                                                                                        tACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCa                     >  2:579686/1‑140 (MQ=255)                                                                                                        tACTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAG‑‑TAGAATTCAAc                   >  2:227686/1‑140 (MQ=255)                                                                                                          cTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAAc                   <  2:1047319/140‑1 (MQ=255)                                                                                                          cTCGCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAAc                   <  2:725458/140‑1 (MQ=255)                                                                                                             gCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAACCTg                >  2:1191364/1‑140 (MQ=255)                                                                                                             gCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAACCTg                >  2:494579/1‑140 (MQ=255)                                                                                                             gCTGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAACCTg                >  1:1346590/1‑140 (MQ=255)                                                                                                               tGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAAt                        >  1:387328/1‑130 (MQ=255)                                                                                                               tGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAAt                        <  2:387328/130‑1 (MQ=255)                                                                                                               tGCCAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCAGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCATCTTCATAGGAAGTGCGGCCCGAAGTATAGAATTCAACCTGAt              >  2:1142843/1‑140 (MQ=255)                                                                                                                 ccAAACCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAACCTGATTg            >  2:89810/1‑140 (MQ=255)                                                                                                                   aaaCCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAACCTGATTggg          <  1:314699/140‑1 (MQ=255)                                                                                                                   aaaCCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAG‑‑TAGAATTCAACCTGATTGGGAt        <  1:227686/140‑1 (MQ=255)                                                                                                                    aaCCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAACCTGATTGGGa         <  2:954652/140‑1 (MQ=255)                                                                                                                     aCCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAACCTGATTGGGAt        <  1:1191364/140‑1 (MQ=255)                                                                                                                     aCCCACGCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAACCTGATTGGGAt        <  1:1634237/140‑1 (MQ=255)                                                                                                                           gCTTGTCGGTCCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAACCTGATTGGGATCACtat  >  1:380150/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                  GTCAGCATGCGAGGGTGGTTTGTACCAGGGTTATGCCCAATGCAAATGACTAAATCGCACTTCTCAAAGTCTTCCAGCAACACGGTCCCTTTACCTACACCGATACTCGCTGCCAAACCCACGCTTGTCGGTTCATGGCACATGTTGGAGCAGTCGGGAAAGTTATTGCTCCCGTATTCACGGGCAAAAAGCTGATAAAGAAAGGCAGCTTCATTGGAAGTGCGGCCCGAAGTATAGAATTCAACCTGATTGGGATCACTAT  >  NC_000913/1585804‑1586065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑