Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,605,416 G→A E122E (GAG→GAA)  lsrB → Autoinducer‑2 ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,605,4160GA100.0% 331.3 / NA 92E122E (GAG→GAA) lsrBAutoinducer‑2 ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (43/49);  total (43/49)

ATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTACTCAAGCCCCACCGTTACGGACCA  >  NC_000913/1605279‑1605550
                                                                                                                                         |                                                                                                                                      
aTTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATg                                                                                                                                      >  1:1018012/1‑140 (MQ=255)
 ttAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATg                                                                                                                                      >  1:988761/1‑139 (MQ=255)
 ttAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGc                                                                                                                                     >  2:383763/1‑140 (MQ=255)
 ttAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGc                                                                                                                                     >  2:368775/1‑140 (MQ=255)
 ttAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGc                                                                                                                                     <  1:231563/140‑1 (MQ=255)
 ttAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGc                                                                                                                                     >  1:1523954/1‑140 (MQ=255)
  taataaCTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGcc                                                                                                                                    >  2:980129/1‑140 (MQ=255)
  taataaCTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGcc                                                                                                                                    >  1:685931/1‑140 (MQ=255)
  taataaCTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGcc                                                                                                                                    <  1:368775/140‑1 (MQ=255)
   aataaCTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTTTGATACTAAACCGGAATGCCg                                                                                                                                   <  2:122443/140‑1 (MQ=255)
   aataaCTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCg                                                                                                                                   <  1:436799/140‑1 (MQ=255)
     taaCTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGct                                                                                                                                 <  1:980129/140‑1 (MQ=255)
     taaCTTCGTCAATCAAGGTTATAACGCCATCATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGct                                                                                                                                 <  2:1018012/140‑1 (MQ=255)
       aCTTCGTCAATCACGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCTATGCAACGTGATGTGAGAGGGCTGACCTGGGACTCTGCTACTAAACCGGAATGCCGctct                                                                                                                               >  2:10228/1‑140 (MQ=255)
       aCTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGctct                                                                                                                               >  1:52892/1‑140 (MQ=255)
       aCTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGctct                                                                                                                               >  1:400142/1‑140 (MQ=255)
         ttCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTta                                                                                                                             <  1:849605/140‑1 (MQ=255)
         ttCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTta                                                                                                                             <  1:738475/140‑1 (MQ=255)
          tCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTtac                                                                                                                            >  2:554078/1‑140 (MQ=255)
           cGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTtact                                                                                                                           >  2:1072440/1‑140 (MQ=255)
             tcaatcaaGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTtactac                                                                                                                         >  1:1646576/1‑140 (MQ=255)
              caatcaaGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACa                                                                                                                        <  1:1657999/140‑1 (MQ=255)
              caatcaaGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACa                                                                                                                        <  2:243074/140‑1 (MQ=255)
              caatcaaGGTTATAACGCCATTATCGTTTCTGCGGCTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACa                                                                                                                        <  2:1144348/140‑1 (MQ=255)
                atcaaGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACAtt                                                                                                                      >  1:1232976/1‑140 (MQ=255)
                   aaGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAAt                                                                                                                   <  1:244055/140‑1 (MQ=255)
                          taACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGaa                                                                                                            >  1:1251836/1‑140 (MQ=255)
                               ccATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACa                                                                                                                        >  2:1407548/1‑123 (MQ=255)
                               ccATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACa                                                                                                                        <  1:1407548/123‑1 (MQ=255)
                               ccATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAAcgccc                                                                                                       >  1:910438/1‑140 (MQ=255)
                               ccATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAAcgccc                                                                                                       >  2:440194/1‑140 (MQ=255)
                               ccATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAAcgccc                                                                                                       >  1:1529099/1‑140 (MQ=255)
                                cATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGaa                                                                                                            <  2:1511839/134‑1 (MQ=255)
                                cATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGaa                                                                                                            >  1:1511839/1‑134 (MQ=255)
                                cATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAAcgcccg                                                                                                      >  1:1439684/1‑140 (MQ=255)
                                cATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAAcgcccg                                                                                                      <  2:910438/140‑1 (MQ=255)
                                cATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAAcgcccg                                                                                                      >  2:646638/1‑140 (MQ=255)
                                cATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAAcgcccg                                                                                                      <  2:52892/140‑1 (MQ=255)
                                cATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAAcgcccg                                                                                                      <  1:440194/140‑1 (MQ=255)
                                cATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAAcgcccg                                                                                                      <  2:1439684/140‑1 (MQ=255)
                                  ttATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAAcgcccgcc                                                                                                    <  2:171559/140‑1 (MQ=255)
                                  ttATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAAcgcccgcc                                                                                                    <  2:1085132/140‑1 (MQ=255)
                                  ttATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAAcgcccgcc                                                                                                    <  1:646638/140‑1 (MQ=255)
                                  ttATCGTTTCTGCGGTTTCACCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAAcgcccgcc                                                                                                    <  1:1187114/140‑1 (MQ=255)
                                   tATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACgcccgccc                                                                                                   >  1:1479329/1‑140 (MQ=255)
                                    aTCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCa                                                                                                  <  1:880075/140‑1 (MQ=255)
                                        tttCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAAcgcccgcc                                                                                                    <  1:270677/134‑1 (MQ=255)
                                        tttCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAAcgcccgcc                                                                                                    >  2:270677/1‑134 (MQ=255)
                                        tttCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTa                                                                                              <  2:400142/140‑1 (MQ=255)
                                            tGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAAcgcccgcc                                                                                                    <  2:974161/130‑1 (MQ=255)
                                            tGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAAcgcccgcc                                                                                                    >  1:974161/1‑130 (MQ=255)
                                            tGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTaggag                                                                                          >  1:407081/1‑140 (MQ=255)
                                            tGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTaggag                                                                                          <  2:1640563/140‑1 (MQ=255)
                                             gCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTaggagg                                                                                         <  1:1072440/140‑1 (MQ=255)
                                              cGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGt                                                                                        <  2:407081/140‑1 (MQ=255)
                                              cGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGt                                                                                        <  2:1251836/140‑1 (MQ=255)
                                               ggTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTa                                                                                       <  2:744664/140‑1 (MQ=255)
                                               ggTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTa                                                                                       <  1:487385/140‑1 (MQ=255)
                                               ggTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTa                                                                                       <  2:1232976/140‑1 (MQ=255)
                                               ggTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTa                                                                                       <  2:1479329/140‑1 (MQ=255)
                                                gTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTAt                                                                                      >  1:671676/1‑140 (MQ=255)
                                                 tttCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATg                                                                                     <  2:1529099/140‑1 (MQ=255)
                                                   tCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGtt                                                                                   >  2:259793/1‑140 (MQ=255)
                                                    cGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTtg                                                                                  <  2:193777/140‑1 (MQ=255)
                                                      ccTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTtggt                                                                                <  2:842041/140‑1 (MQ=255)
                                                               ttGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATggc                                                                       >  1:1114020/1‑140 (MQ=255)
                                                                 gtgtCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATggcgg                                                                     >  2:782683/1‑140 (MQ=255)
                                                                 gtgtCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATggcgg                                                                     >  2:1088402/1‑140 (MQ=255)
                                                                         cACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAg                                                             >  2:52874/1‑140 (MQ=255)
                                                                          aCTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAgg                                                            <  2:1545482/140‑1 (MQ=255)
                                                                          aCTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAgg                                                            <  1:383763/140‑1 (MQ=255)
                                                                          aCTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAgg                                                            <  1:259793/140‑1 (MQ=255)
                                                                            tGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTg                                                          >  2:403385/1‑140 (MQ=255)
                                                                                aCGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATa                                                      <  1:1640878/140‑1 (MQ=255)
                                                                                               tggtgTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAg                                       <  2:783038/140‑1 (MQ=255)
                                                                                                  tgtgAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTATTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTcg                                    >  2:1222694/1‑140 (MQ=255)
                                                                                                    tgAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTcgcg                                  <  2:1386186/140‑1 (MQ=255)
                                                                                                         gTGCTGGCCTGGGACTCTGATTCTAAACCGGAAAGGCGGTCTTTCTTCAATAATCAAGGAACGCCCGCCCAGTTAAGAGGTATTTTTGTTGATATTGCGGCGCCGCAGGGTAATAAAAGCAAAGCCAAAGTCGCGttttt                             <  2:685931/140‑1 (MQ=255)
                                                                                                          tGCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTc                            >  1:233846/1‑140 (MQ=255)
                                                                                                           gCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAAcgcccgcc                                                                                                    <  2:1008279/67‑1 (MQ=255)
                                                                                                           gCTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAAcgcccgcc                                                                                                    >  1:1008279/1‑67 (MQ=255)
                                                                                                            cTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTcgcg                                  >  2:1457360/1‑132 (MQ=255)
                                                                                                            cTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTcgcg                                  <  1:1457360/132‑1 (MQ=255)
                                                                                                            cTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTa                          >  2:552668/1‑140 (MQ=255)
                                                                                                            cTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTa                          >  1:442997/1‑140 (MQ=255)
                                                                                                            cTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTa                          >  2:323610/1‑140 (MQ=255)
                                                                                                            cTGACCTGGGACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTa                          >  1:248780/1‑140 (MQ=255)
                                                                                                                     gACTCTGATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTACTCAAGccc                 <  1:554078/140‑1 (MQ=255)
                                                                                                                           gATACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTACTCAAGCCCCACCGt           >  1:1395515/1‑140 (MQ=255)
                                                                                                                             tACTAAACCGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTACTCAAGCCCCACCGTTa         <  2:233846/140‑1 (MQ=255)
                                                                                                                                    ccGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTACTCAAGCCCCACCGTTACGGACCa  <  2:442997/140‑1 (MQ=255)
                                                                                                                                    ccGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTACTCAAGCCCCACCGTTACGGACCa  >  1:1056189/1‑140 (MQ=255)
                                                                                                                                    ccGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTACTCAAGCCCCACCGTTACGGACCa  >  1:563674/1‑140 (MQ=255)
                                                                                                                                    ccGGAATGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTACTCAAGCCCCACCGTTACGGACCa  <  1:403385/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                      
ATTAATAACTTCGTCAATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTACTCAAGCCCCACCGTTACGGACCA  >  NC_000913/1605279‑1605550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑