Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,720,255	T→C	V127A (GTA→GCA)	slyB →	outer membrane lipoprotein SlyB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,720,255	0	T	C	100.0%	291.6 / NA	82	V127A (GTA→GCA)	slyB	outer membrane lipoprotein SlyB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/38);  total (44/38)

GGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTC  >  NC_000913/1720123‑1720391                                                                                                                                     |                                                                                                                                         ggCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGaaa                                                                                                                                   >  2:1579731/1‑140 (MQ=255)   cGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACa                                                                                                                                 >  2:351975/1‑140 (MQ=255)       ctctGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGc                                                                                                                             <  2:551071/140‑1 (MQ=255)       ctctGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGc                                                                                                                             <  1:1579731/140‑1 (MQ=255)       ctctGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGc                                                                                                                             <  2:1273520/140‑1 (MQ=255)        tctGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCa                                                                                                                            >  1:301424/1‑140 (MQ=255)            gCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAacac                                                                                                                        >  1:606787/1‑140 (MQ=255)                cTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACt                                                                                                                       <  1:1081941/137‑1 (MQ=255)                cTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACt                                                                                                                       >  2:1081941/1‑137 (MQ=255)                cTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGt                                                                                                                    >  2:1517800/1‑140 (MQ=255)                 tGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGtt                                                                                                                   >  2:892292/1‑140 (MQ=255)                      cagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAgg                                                                                                                              <  1:301395/124‑1 (MQ=255)                      cagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAgg                                                                                                                              >  2:301395/1‑124 (MQ=255)                      cagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTtctct                                                                                                              >  1:1479400/1‑140 (MQ=255)                      cagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTtctct                                                                                                              >  2:1069023/1‑140 (MQ=255)                        ggCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTtctctc                                                                                                             >  2:1409183/1‑139 (MQ=255)                        ggCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTtctctc                                                                                                             <  1:1409183/139‑1 (MQ=255)                        ggCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTtctct                                                                                                              <  1:1493803/138‑1 (MQ=255)                        ggCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTtctct                                                                                                              >  2:1493803/1‑138 (MQ=255)                           gcTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCggg                                                                                                         >  1:118865/1‑140 (MQ=255)                            cTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGc                                                                                                        <  1:892292/140‑1 (MQ=255)                            cTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGc                                                                                                        <  2:1059922/140‑1 (MQ=255)                            cTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGc                                                                                                        <  1:1517800/140‑1 (MQ=255)                              gTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCa                                                                                                      <  1:1486484/140‑1 (MQ=255)                              gTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCa                                                                                                      <  1:1607517/140‑1 (MQ=255)                                       ggCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGt                                                                                             >  2:126768/1‑140 (MQ=255)                                       ggCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGt                                                                                             <  1:109678/140‑1 (MQ=255)                                       ggCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGt                                                                                             >  2:1461424/1‑140 (MQ=255)                                         cGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTAc                                                                                           >  1:781503/1‑140 (MQ=255)                                         cGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTAc                                                                                           >  2:1053675/1‑140 (MQ=255)                                         cGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTAc                                                                                           >  2:170938/1‑140 (MQ=255)                                              cTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGcc                                                                                      <  2:930505/140‑1 (MQ=255)                                                  tCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCa                                                                                  >  2:550409/1‑140 (MQ=255)                                                   cAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCaa                                                                                 >  1:750854/1‑140 (MQ=255)                                                     gggCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATg                                                                               <  2:781503/140‑1 (MQ=255)                                                                gTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTg                                                                    >  1:713446/1‑140 (MQ=255)                                                                gTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTg                                                                    >  2:512695/1‑140 (MQ=255)                                                                 tGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGa                                                                   >  2:667166/1‑140 (MQ=255)                                                                  gCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGAc                                                                  <  1:1210802/140‑1 (MQ=255)                                                                      tGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGtt                                                              >  1:1459106/1‑140 (MQ=255)                                                                       gAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGttt                                                             >  2:792599/1‑140 (MQ=255)                                                                         aCAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCGACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTtct                                                           <  2:1479400/140‑1 (MQ=255)                                                                         aCAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTtct                                                           <  2:880456/140‑1 (MQ=255)                                                                         aCAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTtct                                                           >  2:241687/1‑140 (MQ=255)                                                                           aaaaCGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTcc                                                         >  2:385933/1‑140 (MQ=255)                                                                           aaaaCGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTcc                                                         >  1:1241875/1‑140 (MQ=255)                                                                            aaaCGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGAGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCcg                                                        <  2:329169/140‑1 (MQ=255)                                                                             aaCGCAGGGTGTCGAGCTGGAAATTTGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCcgc                                                       >  2:1129862/1‑140 (MQ=255)                                                                              aCGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCcgcg                                                      <  2:606787/140‑1 (MQ=255)                                                                              aCGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCcgcg                                                      <  1:1532256/140‑1 (MQ=255)                                                                                  aGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCcgcg                                                      >  1:221785/1‑136 (MQ=255)                                                                                  aGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCcgcg                                                      <  2:221785/136‑1 (MQ=255)                                                                                  aGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTaa                                                  >  2:800989/1‑140 (MQ=255)                                                                                  aGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGGAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTaa                                                  >  1:828677/1‑140 (MQ=255)                                                                                    ggTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAAt                                                >  2:632114/1‑140 (MQ=255)                                                                                         cGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGtt                                           >  1:100688/1‑140 (MQ=255)                                                                                         cGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGtt                                           >  1:905097/1‑140 (MQ=255)                                                                                            gCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGc                                        <  2:301424/140‑1 (MQ=255)                                                                                              tGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCgt                                      <  1:1231537/140‑1 (MQ=255)                                                                                              tGGAAATTCGCAAACACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCgt                                      <  2:1436409/140‑1 (MQ=255)                                                                                                gAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCgtgt                                    <  2:118865/140‑1 (MQ=255)                                                                                                gAAATTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCgtgt                                    >  2:1120433/1‑140 (MQ=255)                                                                                                  aaTTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTgg                                  <  1:1129862/140‑1 (MQ=255)                                                                                                  aaTTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTgg                                  >  1:1610970/1‑140 (MQ=255)                                                                                                   aTTCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGt                                 <  1:632114/140‑1 (MQ=255)                                                                                                    ttCGTAAAGACGATGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTc                                <  1:512695/140‑1 (MQ=255)                                                                                                                 tGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTa                          >  2:1424524/1‑133 (MQ=255)                                                                                                                 tGGTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTa                          <  1:1424524/133‑1 (MQ=255)                                                                                                                  ggTAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACcac                  <  1:550409/140‑1 (MQ=255)                                                                                                                    tAATACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCacac                <  2:750854/140‑1 (MQ=255)                                                                                                                       tACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGcc             >  1:515028/1‑140 (MQ=255)                                                                                                                       tACCATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGcc             <  2:1063005/140‑1 (MQ=255)                                                                                                                         ccATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGcccc           <  1:800989/140‑1 (MQ=255)                                                                                                                         ccATCATGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGcccc           <  2:702203/140‑1 (MQ=255)                                                                                                                          catcatGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGccccc          <  1:667166/140‑1 (MQ=255)                                                                                                                            tcatGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTa                          >  1:76425/1‑122 (MQ=255)                                                                                                                            tcatGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTa                          <  2:76425/122‑1 (MQ=255)                                                                                                                            tcatGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCtt        >  2:978089/1‑140 (MQ=255)                                                                                                                             catGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACg               >  2:1347943/1‑132 (MQ=255)                                                                                                                             catGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACg               <  1:1347943/132‑1 (MQ=255)                                                                                                                             catGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTc       >  1:984548/1‑140 (MQ=255)                                                                                                                             catGGTGGCACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTc       >  1:570299/1‑140 (MQ=255)                                                                                                                                 gtggcACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCAttt   >  1:1119106/1‑140 (MQ=255)                                                                                                                                  tggcACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTc  <  1:956982/140‑1 (MQ=255)                                                                                                                                  tggcACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTc  <  2:1610970/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         GGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTC  >  NC_000913/1720123‑1720391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑