Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,800,084	T→C	intergenic (‑85/+12)	rpmI ← / ← infC	50S ribosomal subunit protein L35/translation initiation factor IF‑3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,800,084	0	T	C	100.0%	186.4 / NA	53	intergenic (‑85/+12)	rpmI/infC	50S ribosomal subunit protein L35/translation initiation factor IF‑3
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/28);  total (25/28)

TTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCTACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGCCAGTTCTTGCAAATCGTCTTTCACGCGATTAAGCACTTCCATACCG  >  NC_000913/1799951‑1800219                                                                                                                                      |                                                                                                                                        tttttttGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGa                                                                                                                                   <  2:1311380/140‑1 (MQ=255)         aaGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTa                                                                                                                           <  2:511579/140‑1 (MQ=255)          aGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTAc                                                                                                                          <  2:1263976/140‑1 (MQ=255)             gcTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGt                                                                                                                       <  1:1525704/140‑1 (MQ=255)             gcTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGt                                                                                                                       <  2:466670/140‑1 (MQ=255)              cTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGtt                                                                                                                      >  2:1200043/1‑140 (MQ=255)              cTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGtt                                                                                                                      >  1:432342/1‑140 (MQ=255)               ttAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGttt                                                                                                                     <  1:689456/140‑1 (MQ=255)               ttAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGttt                                                                                                                     <  1:1200043/140‑1 (MQ=255)               ttAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGttt                                                                                                                     <  1:622000/140‑1 (MQ=255)                 agcagcACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTttct                                                                                                                   <  1:1578737/140‑1 (MQ=255)                      caCCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTa                                                                                                              <  1:546354/140‑1 (MQ=255)                      caCCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTa                                                                                                              >  1:268743/1‑140 (MQ=255)                         cgcgTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGga                                                                                                           >  1:1060816/1‑140 (MQ=255)                         cgcgTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGga                                                                                                           >  2:440785/1‑140 (MQ=255)                           cgTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGgagc                                                                                                         >  2:257114/1‑140 (MQ=255)                            gTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGgagcg                                                                                                        <  1:257114/140‑1 (MQ=255)                            gTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGACGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGgagcg                                                                                                        <  2:847462/140‑1 (MQ=255)                             tACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGgagcga                                                                                                       <  2:53658/140‑1 (MQ=255)                             tACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGgagcga                                                                                                       >  2:1569920/1‑140 (MQ=255)                             tACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGgagcga                                                                                                       <  2:1324812/140‑1 (MQ=255)                                ggTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCa                                                                                                    <  2:1060816/140‑1 (MQ=255)                                ggTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCCTACTGTTTCTTCTTAGGAGCGAGCa                                                                                                    <  1:790427/140‑1 (MQ=255)                                     tAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATg                                                                                               >  2:462765/1‑140 (MQ=255)                                            tGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCa                                                                                                    <  1:1121227/128‑1 (MQ=255)                                            tGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCa                                                                                                    >  2:1121227/1‑128 (MQ=255)                                            tGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCt                                                                                        >  2:984717/1‑140 (MQ=255)                                            tGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCt                                                                                        >  2:746077/1‑140 (MQ=255)                                            tGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCt                                                                                        >  2:1096293/1‑140 (MQ=255)                                                    taataaCTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCt                                                                                <  2:1490758/140‑1 (MQ=255)                                                         aCTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGAt                                                                           <  1:1066353/140‑1 (MQ=255)                                                          cTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATc                                                                          <  2:1518522/140‑1 (MQ=255)                                                             ccACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTc                                                                       >  1:603472/1‑140 (MQ=255)                                                                    gCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGa                                                                >  2:1075527/1‑140 (MQ=255)                                                                    gCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGa                                                                >  2:908254/1‑140 (MQ=255)                                                                    gCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGa                                                                >  1:42392/1‑140 (MQ=255)                                                                     cATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGaa                                                               >  2:626638/1‑140 (MQ=255)                                                                                    aaaCAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGc                                                >  2:854666/1‑140 (MQ=255)                                                                                    aaaCAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGc                                                >  1:1337784/1‑140 (MQ=255)                                                                                    aaaCAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGc                                                >  2:254600/1‑140 (MQ=255)                                                                                    aaaCAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGc                                                <  1:440785/140‑1 (MQ=255)                                                                                          aGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGAcc                                                     >  1:488789/1‑129 (MQ=255)                                                                                          aGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGAcc                                                     <  2:488789/129‑1 (MQ=255)                                                                                                          tgAAGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGCCAGTTCTTGCAAATCGTCTTTc                          >  1:920356/1‑140 (MQ=255)                                                                                                             aGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGCCAGTTCTTGCAAATCGTCTTTCAcg                       <  1:626638/140‑1 (MQ=255)                                                                                                             aGCCCGAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGCCAGTTCTTGCAAATCGTCTTTCAcg                       <  2:1337784/140‑1 (MQ=255)                                                                                                                  gAAGGCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGCCAGTTCTTGCAAATCGTCTTTCACGCGAtt                  >  1:212065/1‑140 (MQ=255)                                                                                                                     ggCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGCCAGTTCTTGCAAATCGTCTTTCACGCGATTAAg               <  2:268743/140‑1 (MQ=255)                                                                                                                     ggCTCCACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGCCAGTTCTTGCAAATCGTCTTTCACGCGATTAAg               <  1:254600/140‑1 (MQ=255)                                                                                                                         ccACAGACAGTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGCCAGTTCTTGCAAATCGTCTTTCACGCGATTAAGCACt           >  1:647340/1‑140 (MQ=255)                                                                                                                                  gTGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGCCAGTTCTTGCAAATCGTCTTTCACGCGATTAAGCACTTCCATACCg  >  1:669448/1‑140 (MQ=255)                                                                                                                                   tGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGCCAGTTCTTGCAAATCGTCTTTCACGCGATTAAg               <  2:873097/126‑1 (MQ=255)                                                                                                                                   tGCCACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGCCAGTTCTTGCAAATCGTCTTTCACGCGATTAAg               <  1:1060565/126‑1 (MQ=255)                                                                                                                                      |                                                                                                                                        TTTTTTTGAAGCGCTTAGCAGCACCGCGTACGGTCTTAATTTTTGGCATTTTAATAACTTCCACTTCGCATTGTTAATAAACGAAACAAAGGCGAACAAAGCCTGTGAAGCCCGAAGGCTCCACAGACAGTGCTACTTGAAGGCCTTACTGTTTCTTCTTAGGAGCGAGCACCATGATCATCTGGCGGCCTTCGATCTTCGTTGGGAAGGATTCGACCACTGCCAGTTCTTGCAAATCGTCTTTCACGCGATTAAGCACTTCCATACCG  >  NC_000913/1799951‑1800219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑