Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,145,623	T→C	S786S (AGT→AGC)	dgcE →	putative diguanylate cyclase DgcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,145,623	0	T	C	100.0%	292.7 / NA	82	S786S (AGT→AGC)	dgcE	putative diguanylate cyclase DgcE
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/49);  total (33/49)

ACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGTGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCGCAGGCTGATATCGCCTGTTATGCC  >  NC_000913/2145491‑2145758                                                                                                                                     |                                                                                                                                        aCTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGcc                                                                                                                                      <  2:1142242/136‑1 (MQ=255) aCTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGcc                                                                                                                                      >  1:1142242/1‑136 (MQ=255) aCTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGa                                                                                                                                  <  1:1015432/140‑1 (MQ=255) aCTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGa                                                                                                                                  <  2:321861/140‑1 (MQ=255)        tcgtTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTa                                                                                                                           <  1:467584/140‑1 (MQ=255)         cgtTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTAt                                                                                                                          <  2:1190386/140‑1 (MQ=255)         cgtTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTAt                                                                                                                          <  1:1481238/140‑1 (MQ=255)         cgtTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTAt                                                                                                                          <  1:1429700/140‑1 (MQ=255)         cgtTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTAt                                                                                                                          <  1:1304445/140‑1 (MQ=255)           ttAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCa                                                                                                                        <  1:543619/140‑1 (MQ=255)             aaTGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACt                                                                                                                      <  2:835445/140‑1 (MQ=255)                gCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTta                                                                                                                   <  2:828900/140‑1 (MQ=255)                     gTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATgg                                                                                                              >  1:981183/1‑140 (MQ=255)                     gTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATgg                                                                                                              >  1:1127775/1‑140 (MQ=255)                      tATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATggg                                                                                                             <  2:136000/140‑1 (MQ=255)                       aTGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGa                                                                                                            <  2:1561364/140‑1 (MQ=255)                       aTGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGa                                                                                                            <  2:55315/140‑1 (MQ=255)                         gctgcGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAg                                                                                                          >  1:754056/1‑140 (MQ=255)                           tgcGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGc                                                                                                        >  2:737841/1‑140 (MQ=255)                             cGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCg                                                                                                      <  1:1084344/140‑1 (MQ=255)                             cGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCg                                                                                                      <  2:1127775/140‑1 (MQ=255)                              gTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCgt                                                                                                     >  2:1181781/1‑140 (MQ=255)                              gTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCgt                                                                                                     <  2:371545/140‑1 (MQ=255)                              gTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCgt                                                                                                     >  1:1504007/1‑140 (MQ=255)                               ttCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCgtg                                                                                                    <  2:1504007/140‑1 (MQ=255)                                tCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCgtgt                                                                                                   >  2:608480/1‑140 (MQ=255)                                         gTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGACGTGTGCATCGGGt                                                                                          <  2:1093504/140‑1 (MQ=255)                                          tGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTa                                                                                         <  1:1418861/140‑1 (MQ=255)                                          tGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTa                                                                                         <  1:39204/140‑1 (MQ=255)                                               gcgcGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGc                                                                                    <  1:33629/140‑1 (MQ=255)                                                    aCTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTg                                                                               >  1:9298/1‑140 (MQ=255)                                                     cTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGc                                                                              <  2:1516962/140‑1 (MQ=255)                                                     cTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGc                                                                              <  1:489668/140‑1 (MQ=255)                                                         gTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCggg                                                                          <  1:608480/140‑1 (MQ=255)                                                          tGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGa                                                                         <  1:1181781/140‑1 (MQ=255)                                                          tGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGa                                                                         <  2:636237/140‑1 (MQ=255)                                                               aTGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCa                                                                                  >  1:805772/1‑126 (MQ=255)                                                               aTGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCa                                                                                  <  2:805772/126‑1 (MQ=255)                                                               aTGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTAcc                                                                    >  1:1112961/1‑140 (MQ=255)                                                                tGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCt                                                                   <  1:737841/140‑1 (MQ=255)                                                                    tttGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCCTTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGc                                                                              <  2:1507051/125‑1 (MQ=255)                                                                    tttGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGc                                                                              >  1:1507051/1‑125 (MQ=255)                                                                        gTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTtgattgat                                                           >  2:592503/1‑140 (MQ=255)                                                                            gctgctACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGaca                                                       >  1:868075/1‑140 (MQ=255)                                                                             ctgctACCAGACTGTAATGTTGAAAGCGCGCGTGTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGacaa                                                      <  2:1464974/140‑1 (MQ=255)                                                                                  aCCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAatc                                                 >  2:1197847/1‑140 (MQ=255)                                                                                       aCTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATtgatga                                                         >  1:1081094/1‑127 (MQ=255)                                                                                       aCTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATtgatga                                                         <  2:1081094/127‑1 (MQ=255)                                                                                       aCTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAg                                            >  1:394815/1‑140 (MQ=255)                                                                                        cTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAgg                                           <  1:535018/140‑1 (MQ=255)                                                                                          gTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTGCCTTGATTGATGACAACAATCATCAggcg                                         >  2:520348/1‑140 (MQ=255)                                                                                          gTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAggcg                                         >  1:1311534/1‑140 (MQ=255)                                                                                          gTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAggca                                         >  2:402646/1‑139 (MQ=255)                                                                                          gTAATGTTGAAAGCGCGCGTTTTATCGCTACAAGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAggcg                                         >  2:758771/1‑140 (MQ=255)                                                                                           tAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAggcgg                                        >  2:709705/1‑140 (MQ=255)                                                                                            aaTGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAggcggc                                       <  1:709705/140‑1 (MQ=255)                                                                                                 tGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAg                                  >  1:583267/1‑140 (MQ=255)                                                                                                   aaaGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTGCCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTg                                <  1:520348/140‑1 (MQ=255)                                                                                                   aaaGCGCGCGATTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAAGCGGCAGAAGTg                                >  2:1596129/1‑140 (MQ=255)                                                                                                     aGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGAt                              >  1:1592734/1‑140 (MQ=255)                                                                                                     aGCGCGCGTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGAt                              >  1:408427/1‑140 (MQ=255)                                                                                                        gcgcgTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTc                           <  2:394815/140‑1 (MQ=255)                                                                                                         cgcgTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCg                          >  1:1548937/1‑140 (MQ=255)                                                                                                         cgcgTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCg                          <  2:868075/140‑1 (MQ=255)                                                                                                         cgcgTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCg                          <  2:981183/140‑1 (MQ=255)                                                                                                         cgcgTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCg                          <  2:1040468/140‑1 (MQ=255)                                                                                                         cgcgTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCg                          >  2:1365483/1‑140 (MQ=255)                                                                                                          gcgTTTTATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCGc                         >  2:1354047/1‑140 (MQ=255)                                                                                                             ttttATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCg                          >  2:1268301/1‑136 (MQ=255)                                                                                                             ttttATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCg                          <  1:1268301/136‑1 (MQ=255)                                                                                                             ttttATCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCGCAgg                      <  1:1354047/140‑1 (MQ=255)                                                                                                                  tCGCTACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCGCAGGCTGat                 <  2:9298/140‑1 (MQ=255)                                                                                                                      tACACGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCGCAGGCTGATATCg             <  2:754056/140‑1 (MQ=255)                                                                                                                      tACAAGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCGCAGGCTGATATCg             <  1:758771/140‑1 (MQ=255)                                                                                                                        cacGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCGCAGGCTGATATCGcc           >  1:165938/1‑140 (MQ=255)                                                                                                                        cacGCATTATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCGCAGGCTGATATCGcc           >  2:695832/1‑140 (MQ=255)                                                                                                                              ttATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCGCAGGCTGATATCGCCTGTTAt     <  2:165938/140‑1 (MQ=255)                                                                                                                               tATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCGCAGGCTGATATCGCCTGTTATg    >  2:691321/1‑140 (MQ=255)                                                                                                                               tATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCGCAGGCTGATATCGCCTGTTATg    <  2:1591491/140‑1 (MQ=255)                                                                                                                               tATCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCGCAGGCTGATATCGCCTGTTATg    <  1:1596129/140‑1 (MQ=255)                                                                                                                                aTCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCGCAGGCTGATATCGCCTGTTATGc   >  2:679807/1‑140 (MQ=255)                                                                                                                                 tCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCGCAGGCTGATATCGCCTGTTATGcc  <  1:1364992/140‑1 (MQ=255)                                                                                                                                 tCAGCGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCGCAGGCTGATATCGCCTGTTATGcc  <  2:408427/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        ACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGTGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCGCAGGCTGATATCGCCTGTTATGCC  >  NC_000913/2145491‑2145758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑