Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,195,760	G→A	V487V (GTG→GTA)	metG →	methionine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,195,760	0	G	A	100.0%	286.8 / NA	81	V487V (GTG→GTA)	metG	methionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/45);  total (36/45)

GTGCGCGAAATCATGGCGCTGGCTGATCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCT  >  NC_000913/2195623‑2195891                                                                                                                                          |                                                                                                                                    gTGCGCGAAATCATGGCGCTGGCTGATCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACt                                                                                                                                   <  1:280259/140‑1 (MQ=255)  tGCGCGAAATCATGGCGCTGGCTGATCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACtg                                                                                                                                  >  1:561696/1‑140 (MQ=255)  tGCGCGAAATCATGGCGCTGGCTGATCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACtg                                                                                                                                  >  2:1587687/1‑140 (MQ=255)  tGCGCGAAATCATGGCGCTGGCTGATCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACtg                                                                                                                                  <  2:1421681/140‑1 (MQ=255)   gcgcGAAATCATGGCGCTGGCTGATCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACtga                                                                                                                                 >  1:1620269/1‑140 (MQ=255)   gcgcGAAATCATGGCGCTGGCTGATCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACtga                                                                                                                                 >  1:32075/1‑140 (MQ=255)   gcgcGAAATCATGGCGCTGGCTGATCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACtga                                                                                                                                 <  1:922644/140‑1 (MQ=255)    cgcgAAATCATGGCGCTGGCTGATCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACtgat                                                                                                                                >  2:161936/1‑140 (MQ=255)             aTGGCGCTGGCTGATCTGGCTAACCGCTATGTCGATGAACTGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCACTGGGCATCAACCTGTTCCGCGTACTGATGACTTACCt                                                                                                                       <  2:1466448/140‑1 (MQ=255)             aTGGCGCTGGCTGATCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCt                                                                                                                       <  1:1587687/140‑1 (MQ=255)                  gctggctgATCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCATCCTGGTCCGCGTACTGATGACTTACCTGAAGc                                                                                                                  <  2:1268157/140‑1 (MQ=255)                  gctggctgATCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGc                                                                                                                  <  1:335277/140‑1 (MQ=255)                   ctggctgATCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGcc                                                                                                                 >  1:1338146/1‑140 (MQ=255)                     ggctgATCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCgg                                                                                                               >  2:1159852/1‑140 (MQ=255)                      gctgATCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGt                                                                                                              <  2:32075/140‑1 (MQ=255)                      gctgATCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGt                                                                                                              <  2:54685/140‑1 (MQ=255)                             tGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGt                                                                                                              <  1:818149/133‑1 (MQ=255)                             tGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGt                                                                                                              >  2:818149/1‑133 (MQ=255)                                cTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGaaa                                                                                                    >  2:1381786/1‑140 (MQ=255)                                cTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGaaa                                                                                                    >  1:1087014/1‑140 (MQ=255)                                     cGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGAc                                                                                               <  2:561696/140‑1 (MQ=255)                                        tATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGa                                                                                            <  1:1159852/140‑1 (MQ=255)                                             cGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTg                                                                                       <  2:179072/140‑1 (MQ=255)                                              gATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGc                                                                                      >  2:655538/1‑140 (MQ=255)                                               aTGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCa                                                                                     >  2:248819/1‑140 (MQ=255)                                                tGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAg                                                                                    <  1:655538/140‑1 (MQ=255)                                                  aaCAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGaa                                                                                  >  1:1096533/1‑140 (MQ=255)                                                   aCAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAg                                                                                 <  1:1480194/140‑1 (MQ=255)                                                    cAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAg                                                                                 <  1:724556/139‑1 (MQ=255)                                                    cAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAg                                                                                 >  2:724556/1‑139 (MQ=255)                                                     aGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCa                                                                               <  1:181519/140‑1 (MQ=255)                                                      ggCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCAt                                                                              >  1:1156863/1‑140 (MQ=255)                                                      ggCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCAt                                                                              >  2:1136941/1‑140 (MQ=255)                                                       gCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGAc                                                                                               <  1:1168343/122‑1 (MQ=255)                                                       gCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGAc                                                                                               >  2:1168343/1‑122 (MQ=255)                                                       gCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCAtt                                                                             >  1:821519/1‑140 (MQ=255)                                                          ccGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCt                                                                          <  1:1322854/140‑1 (MQ=255)                                                          ccGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCt                                                                          <  2:1096533/140‑1 (MQ=255)                                                          ccGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCt                                                                          <  1:1136941/140‑1 (MQ=255)                                                          ccGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCt                                                                          <  2:1338146/140‑1 (MQ=255)                                                          ccGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCt                                                                          <  2:711689/140‑1 (MQ=255)                                                            gtgggtggTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCa                                                                        >  1:1642414/1‑140 (MQ=255)                                                               ggtggtGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATa                                                                     <  1:1248980/140‑1 (MQ=255)                                                                 tggtggCGAAACAGGAAGGCCGCGATGCCGCCCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCa                                                                               <  2:1139629/128‑1 (MQ=255)                                                                 tggtggCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAg                                                                                 >  2:1417989/1‑126 (MQ=255)                                                                 tggtggCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAg                                                                                 <  1:1417989/126‑1 (MQ=255)                                                                 tggtggCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCa                                                                               <  2:1010015/128‑1 (MQ=255)                                                                 tggtggCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCa                                                                               >  1:1010015/1‑128 (MQ=255)                                                                 tggtggCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACg                                                                   >  2:1635314/1‑140 (MQ=255)                                                                 tggtagCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCa                                                                               >  1:1139629/1‑128 (MQ=255)                                                                    tggCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGaa                                                                <  1:1146007/140‑1 (MQ=255)                                                                    tggCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGaa                                                                <  1:508736/140‑1 (MQ=255)                                                                    tggCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGaa                                                                <  2:831181/140‑1 (MQ=255)                                                                      gCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACt                                                              <  1:161936/140‑1 (MQ=255)                                                                         aaaCAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGAc                                                           >  2:136719/1‑140 (MQ=255)                                                                          aaCAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGAcc                                                          <  1:1635314/140‑1 (MQ=255)                                                                             aGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTgg                                                       >  2:1088461/1‑140 (MQ=255)                                                                               gAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGaa                                                                >  2:1153912/1‑129 (MQ=255)                                                                               gAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGaa                                                                <  1:1153912/129‑1 (MQ=255)                                                                                aaGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGaa                                                                >  2:543745/1‑128 (MQ=255)                                                                                aaGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGaa                                                                <  1:543745/128‑1 (MQ=255)                                                                                 aGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATg                                                   <  2:1422240/140‑1 (MQ=255)                                                                                   gCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGa                                                     <  2:1273913/136‑1 (MQ=255)                                                                                   gCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGa                                                     >  1:1273913/1‑136 (MQ=255)                                                                                     cgcgATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTAt                                               >  1:1604445/1‑140 (MQ=255)                                                                                     cgcgATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTAt                                               >  1:116146/1‑140 (MQ=255)                                                                                             cGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAAc                                       <  2:1620269/140‑1 (MQ=255)                                                                                              gACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAAcc                                      >  1:983748/1‑140 (MQ=255)                                                                                                    cAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgctgct                                >  1:1068094/1‑140 (MQ=255)                                                                                                        cAATTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGc                            <  2:116146/140‑1 (MQ=255)                                                                                                          aTTTGCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCca                          <  1:1381786/140‑1 (MQ=255)                                                                                                              gCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGc                            <  2:1407072/134‑1 (MQ=255)                                                                                                              gCTCAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGc                            >  1:1407072/1‑134 (MQ=255)                                                                                                                 cAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACaa                       <  2:25606/136‑1 (MQ=255)                                                                                                                 cAATGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACaa                       >  1:25606/1‑136 (MQ=255)                                                                                                                   aTGGGCATCAACCTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGaa                 <  1:1088461/140‑1 (MQ=255)                                                                                                                              ccTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAgg      <  1:1021671/140‑1 (MQ=255)                                                                                                                               cTGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGgc     <  1:136719/140‑1 (MQ=255)                                                                                                                                tGTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGgcg    <  1:248819/140‑1 (MQ=255)                                                                                                                                cgTTCCGCGTACTGATGACTTGCCTGAACCCGGTACTGCAGAACCTGACTGAGCGTGCACAAGCATTCCTCAATACGGAACTGCCCTGGCACCGTATCCAGCAACCCCTGCTGGGCCACAAAGTGAATCCGTTCAAGgcg    <  2:983748/139‑1 (MQ=255)                                                                                                                                 gTTCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGgcgc   >  1:862515/1‑140 (MQ=255)                                                                                                                                  ttCCGCGTACTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCt  >  1:128620/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                    GTGCGCGAAATCATGGCGCTGGCTGATCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCT  >  NC_000913/2195623‑2195891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑