| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,507,458 | T→C | N146S (AAC→AGC) | fryC ← | putative PTS enzyme IIC component FryC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,507,458 | 0 | T | C | 98.2% | 184.5 / ‑4.7 | 55 | N146S (AAC→AGC) | fryC | putative PTS enzyme IIC component FryC |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (51/3); total (52/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
ACAGGAAATCCGGTAACTGGATTTTCTGGTTCATCCACTTCACAAGGTAACCGGCAACGATAGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAT‑CGGAATGGCAAAACCAAACAACACGCCGCCGAAGG > NC_000913/2507321‑2507593 | aCAGGAAATCCGGTAACTGGATTTTCTGGTTCATCCACTTCACAAGGTAACCGGCAACGATAGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTc > 2:1247821/1‑140 (MQ=255) tCCGGTAACTGGATTTTCTGGTTCATCCACTTCACAAGGTAACCGGCAACGATAGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTg > 1:1471622/1‑140 (MQ=255) ggTAACTGGATTTTCTGGTTCATCCACTTCACAAGGTAACCGGCAACGATAGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATCGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGt < 2:1471622/140‑1 (MQ=255) ggATTTTCTGGTTCATCCACCTCACAAGGTAACCGGCAACGATAGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTgg < 2:556384/140‑1 (MQ=255) aTTTTCTGGTTCATCCACTTCACAAGGTAACCGGCAACGATAGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGt < 2:768977/140‑1 (MQ=255) ggTTCATCCACTTCACAAGGTAACCGGCAACGATAGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATc > 1:590013/1‑140 (MQ=255) tCATCCACTTCACAAGGTAACCGGTAACGATAGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAAc > 1:992898/1‑140 (MQ=255) cATCCACTTCACAAGGTAACCGGCAACGATAGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAAcc > 1:1490953/1‑140 (MQ=255) ccACTTCACAAGGTAACCGGCAACGATAGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACccgc > 1:1325227/1‑140 (MQ=255) tCACAAGGTAACCGGCAACGATAGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATa > 1:217758/1‑140 (MQ=255) ggTAACCGGCAACGATAGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCa > 1:1057908/1‑140 (MQ=255) aaCCGGCAACGATAGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGcc > 2:1430598/1‑140 (MQ=255) ccGGCAACGATAGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCgg > 1:577940/1‑140 (MQ=255) aaCGATAGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACg > 1:403210/1‑140 (MQ=255) cGATAGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGcc > 2:968449/1‑140 (MQ=255) aaaaatCAGCGCACAAAGGAGGGGGGCGGGCCCGGGAGAGGGGGCCCCCCTTTGAATGGTGGTGGGATCGAACCTCAGCGGTTGCGGGGGCGGGGAAACCACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGc < 2:1117774/137‑1 (MQ=255) aaaaatCAGCCCACAAGGGAGGGGGGAGGGCCCGGGGGAAGGGGCCGCCTTTTGTATGGTGGGGGAATCGAAGCTCGGCGTTTGCGGGGGGGGGGAAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGc < 2:577940/137‑1 (MQ=255) atCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCAcc > 2:597777/1‑140 (MQ=255) atCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCAcc > 1:791092/1‑140 (MQ=255) atCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCAcc > 2:182074/1‑140 (MQ=255) cAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGa > 1:103784/1‑140 (MQ=255) cgcACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATag > 1:1117774/1‑140 (MQ=255) cgcACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATag > 1:1529902/1‑140 (MQ=255) cgcACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATag > 2:954482/1‑140 (MQ=255) cgcACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATag > 1:903605/1‑140 (MQ=255) cgcACCAATGAAGGTGGACGGAACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATag > 2:1179118/1‑140 (MQ=255) cACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATagag > 1:334045/1‑140 (MQ=255) cAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTc > 1:1126416/1‑140 (MQ=255) aTGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGc > 1:4624/1‑140 (MQ=255) ggTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaa > 2:1047467/1‑140 (MQ=255) ggACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAag > 1:869494/1‑140 (MQ=255) ggACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAag > 1:818816/1‑140 (MQ=255) ggACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAag > 1:1578410/1‑140 (MQ=255) gACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAagc > 1:743279/1‑140 (MQ=255) aCGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAagca > 2:975506/1‑140 (MQ=255) aCGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAagca > 2:756807/1‑140 (MQ=255) cACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCaa > 1:1354026/1‑140 (MQ=255) cACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCaa > 1:1369377/1‑140 (MQ=255) aCCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCaaa > 2:1447379/1‑140 (MQ=255) cGCCCATTGCATAGCGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAT‑CGGAATGGCaa > 2:1579201/1‑140 (MQ=255) gTGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAT‑CGGAATGGCAAAACCAAACAacac > 1:1146051/1‑140 (MQ=255) tGCTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAT‑CGGAATGGCAAAACCAAACAACAcg > 1:1063428/1‑140 (MQ=255) cTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAT‑CGGAATGGCAAAACCAAACAACAcgcc > 1:280000/1‑140 (MQ=255) cTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAA‑CAACCAACATCCGGAATGG‑AAAACCCAAAAAAAcgccc > 2:1214180/1‑139 (MQ=255) cTGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGAT‑AAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAT‑CGGAATGGCAAAACCAAACAACAcgccg > 1:55187/1‑140 (MQ=255) tGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAT‑CGGAATGGCAAAACCAAACAACAcgccg > 2:1454981/1‑140 (MQ=255) tGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAT‑CGGAATGGCAAAACCAAACAACAcgccg > 2:160294/1‑140 (MQ=255) tGGGATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAT‑CGGAATGGCAAAACCAAACAACAcgccg > 1:1445523/1‑140 (MQ=255) ggATCGAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAT‑CGGAATGGCAAAACCAAACAACACgccgcc > 2:558327/1‑140 (MQ=255) gAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAT‑CGGAATGGCAAAACCAAACAACACGCCGCCGAAgg > 2:27263/1‑140 (MQ=255) gAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAT‑CGGAATGGCAAAACCAAACAACACGCCGCCGAAgg > 2:789512/1‑140 (MQ=255) gAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAT‑CGGAATGGCAAAACCAAACAACACGCCGCCGAAgg > 1:1505303/1‑140 (MQ=255) gAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAT‑CGGAATGGCAAAACCAAACAACACGCCGCCGAAgg > 1:36743/1‑140 (MQ=255) gAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAT‑CGGAATGGCAAAACCAAACAACACGCCGCCGAAgg > 2:1216477/1‑140 (MQ=255) gAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAT‑CGGAATGGCAAAACCAAACAACACGCCGCCGAAgg > 2:1629483/1‑140 (MQ=255) gAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAT‑CGGAATGGCAAAACCAAACAACACGCCGCCGAAgg > 1:157928/1‑140 (MQ=255) gAAGCTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAT‑CGGAATGGCAAAACCAAACAACACGCCGCCGAAgg > 2:1376322/1‑140 (MQ=255) | ACAGGAAATCCGGTAACTGGATTTTCTGGTTCATCCACTTCACAAGGTAACCGGCAACGATAGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAT‑CGGAATGGCAAAACCAAACAACACGCCGCCGAAGG > NC_000913/2507321‑2507593 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |