Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,538,274	A→G	T176A (ACT→GCT)	yfeS →	conserved protein YfeS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,538,274	0	A	G	100.0%	326.1 / NA	91	T176A (ACT→GCT)	yfeS	conserved protein YfeS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/47);  total (44/47)

CCGTAAAGAGCCGGATCTGGACTGTGCTGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCACTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATG  >  NC_000913/2538138‑2538410                                                                                                                                         |                                                                                                                                         ccGTAAAGAGCCGGATCTGGACTGTGCTGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTa                                                                                                                                       >  2:236632/1‑140 (MQ=255) ccGTAAAGAGCCGGATCTGGACTGTGCTGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTa                                                                                                                                       >  2:1372481/1‑140 (MQ=255)      aaGAGCCGGATCTGGACTGTGCTGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCag                                                                                                                                  <  2:1032184/140‑1 (MQ=255)      aaGAGCCGGATCTGGACTGTGCTGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCag                                                                                                                                  <  1:1439136/140‑1 (MQ=255)        gagCCGGATCTGGACTGTGCTGATTTTCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCagag                                                                                                                                <  2:1567774/140‑1 (MQ=255)         agCCGGATCTGGACTGTGCTGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGc                                                                                                                               <  2:1509973/140‑1 (MQ=255)           ccGGATCTGGACTGTGCTGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCaa                                                                                                                             >  1:1016752/1‑140 (MQ=255)            cGGATCTGGACTGTGCTGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAat                                                                                                                            <  2:863716/140‑1 (MQ=255)            cGGATCTGGACTGTGCTGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAat                                                                                                                            >  1:755022/1‑140 (MQ=255)                tCTGGACTGTGCTGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGa                                                                                                                        <  2:1562495/140‑1 (MQ=255)                tCTGGACTGTGCTGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGa                                                                                                                        <  1:136639/140‑1 (MQ=255)                tCTGGACTGTGCTGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGa                                                                                                                        <  1:191784/140‑1 (MQ=255)                         tgCTGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTa                                                                                                                 >  1:430822/1‑138 (MQ=255)                         tgCTGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTa                                                                                                                 >  1:345730/1‑138 (MQ=255)                         tgCTGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTa                                                                                                                 <  2:345730/138‑1 (MQ=255)                         tgCTGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTa                                                                                                                 <  2:430822/138‑1 (MQ=255)                         tgCTGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACg                                                                                                               >  1:101097/1‑140 (MQ=255)                         tgCTGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACg                                                                                                               >  1:175920/1‑140 (MQ=255)                         tgCTGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACg                                                                                                               >  2:204561/1‑140 (MQ=255)                         tgCTGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACg                                                                                                               >  2:296101/1‑140 (MQ=255)                         tgCTGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACg                                                                                                               >  2:427889/1‑140 (MQ=255)                            tGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCaa                                                                                                            <  2:588452/140‑1 (MQ=255)                            tGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCaa                                                                                                            <  1:1138476/140‑1 (MQ=255)                            tGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCaa                                                                                                            <  1:296101/140‑1 (MQ=255)                             gATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAAc                                                                                                           <  1:306794/140‑1 (MQ=255)                              aTTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACt                                                                                                          >  1:1292244/1‑140 (MQ=255)                                   ccTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCAtt                                                                                                     >  1:666859/1‑140 (MQ=255)                                   ccTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCAtt                                                                                                     <  1:210202/140‑1 (MQ=255)                                   ccTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCAtt                                                                                                     >  1:796796/1‑140 (MQ=255)                                   ccTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCAtt                                                                                                     >  1:1102277/1‑140 (MQ=255)                                      cAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCgg                                                                                                  <  1:1532095/140‑1 (MQ=255)                                      cAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCgg                                                                                                  <  1:204561/140‑1 (MQ=255)                                       aaaTGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGt                                                                                                 <  1:714873/140‑1 (MQ=255)                                       aaaTGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGt                                                                                                 <  1:134076/140‑1 (MQ=255)                                        aaTGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTc                                                                                                <  1:194615/140‑1 (MQ=255)                                                   aaaCTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTc                                                                                     <  2:755022/140‑1 (MQ=255)                                                   aaaCTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTc                                                                                     <  1:1092945/140‑1 (MQ=255)                                                   aaaCTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTc                                                                                     <  1:90787/140‑1 (MQ=255)                                                         tgtgGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTc                                                                                     <  1:365410/134‑1 (MQ=255)                                                         tgtgGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTc                                                                                     >  2:365410/1‑134 (MQ=255)                                                              ggTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTc                                                                                     <  2:602781/129‑1 (MQ=255)                                                              ggTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTc                                                                                     >  1:602781/1‑129 (MQ=255)                                                                tATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAAtct                                                                        >  1:1359707/1‑140 (MQ=255)                                                                   gAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTccc                                                                     >  2:1170327/1‑140 (MQ=255)                                                                     aaTACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCAt                                                                   >  1:157472/1‑140 (MQ=255)                                                                     aaTACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCAt                                                                   <  2:1359707/140‑1 (MQ=255)                                                                        aCATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATaaa                                                                >  1:277382/1‑140 (MQ=255)                                                                          aTCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACt                                                              >  1:1311627/1‑140 (MQ=255)                                                                          aTCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACt                                                              >  1:409029/1‑140 (MQ=255)                                                                            cGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTa                                                            >  1:1310368/1‑140 (MQ=255)                                                                            cGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTa                                                            >  2:304333/1‑140 (MQ=255)                                                                            cGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTa                                                            >  1:1412544/1‑140 (MQ=255)                                                                            cGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTa                                                            >  1:165908/1‑140 (MQ=255)                                                                               tATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTaaaa                                                         >  1:1551206/1‑140 (MQ=255)                                                                               tATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTaaaa                                                         <  1:1170327/140‑1 (MQ=255)                                                                                 tGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAg                                                       >  2:1084043/1‑140 (MQ=255)                                                                                      aGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATggg                                                  <  2:1412544/140‑1 (MQ=255)                                                                                       gTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGa                                                 <  1:1084043/140‑1 (MQ=255)                                                                                        tATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGa                                                 <  2:25203/139‑1 (MQ=255)                                                                                        tATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGa                                                 >  1:25203/1‑139 (MQ=255)                                                                                        tATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGAc                                                >  1:725415/1‑140 (MQ=255)                                                                                        tATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGAc                                                >  2:814949/1‑140 (MQ=255)                                                                                        tATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGAc                                                >  1:1623102/1‑140 (MQ=255)                                                                                         aTTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACt                                               <  1:1372481/140‑1 (MQ=255)                                                                                         aTTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACt                                               <  1:236632/140‑1 (MQ=255)                                                                                              tGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATg                                          <  2:175920/140‑1 (MQ=255)                                                                                                aaGAGGATGTTCGTGCGCGATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCa                                        <  2:157472/140‑1 (MQ=255)                                                                                                  gagGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACt                                               <  1:1604941/131‑1 (MQ=255)                                                                                                  gagGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACt                                               >  2:1604941/1‑131 (MQ=255)                                                                                                  gagGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACt                                      <  2:1102277/140‑1 (MQ=255)                                                                                                  gagGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACt                                      >  1:953495/1‑140 (MQ=255)                                                                                                  gagGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACt                                      >  2:1437767/1‑140 (MQ=255)                                                                                                    ggATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAg                                    <  2:1623102/140‑1 (MQ=255)                                                                                                    ggATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAg                                    >  2:768040/1‑140 (MQ=255)                                                                                                     gATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAgc                                   <  2:165908/140‑1 (MQ=255)                                                                                                      aTGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAgcg                                  <  2:1292244/140‑1 (MQ=255)                                                                                                      aTGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAgcg                                  <  2:1228282/140‑1 (MQ=255)                                                                                                       tGTTCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAgcgc                                 >  2:162452/1‑140 (MQ=255)                                                                                                         ttCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAGCGCGt                               <  2:1311627/140‑1 (MQ=255)                                                                                                         ttCGTGCGCAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAGCGCGt                               <  2:1310368/140‑1 (MQ=255)                                                                                                               cgcAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAGCGCGTCATCAg                         >  1:1463363/1‑140 (MQ=255)                                                                                                               cgcAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAGCGCGTCATCAg                         >  1:1229319/1‑140 (MQ=255)                                                                                                               cgcAATTACTAGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAGCGCGTCATCAg                         >  1:376043/1‑140 (MQ=255)                                                                                                                         aGTCGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCaa               <  2:1016752/140‑1 (MQ=255)                                                                                                                            cGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCaaaaa            >  1:1552972/1‑140 (MQ=255)                                                                                                                            cGATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCaaaaa            >  2:1046589/1‑140 (MQ=255)                                                                                                                             gATGAAATGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAAt           >  1:1603538/1‑140 (MQ=255)                                                                                                                                   aTGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCa     <  2:796796/140‑1 (MQ=255)                                                                                                                                    tGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCaa    <  2:1603538/140‑1 (MQ=255)                                                                                                                                    tGAGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCaa    <  1:768040/140‑1 (MQ=255)                                                                                                                                      aGCGCTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATg  <  1:162452/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CCGTAAAGAGCCGGATCTGGACTGTGCTGATTTCCCTCAAATGTTAATTGAAACTATGTGGGGTATGAAATACATCGCTATGGACAGTATTCTTGAAGAGGATGTTCGTGCGCAATTACTAGTCGATGAAATGAGCACTATCCAGAGCAATATGATTACCTACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATG  >  NC_000913/2538138‑2538410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑