Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,770,027	T→C	S109P (TCT→CCT)	yfjR →	CP4‑57 prophage; putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,770,027	0	T	C	100.0%	299.8 / NA	85	S109P (TCT→CCT)	yfjR	CP4‑57 prophage; putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (40/45);  total (40/45)

CTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGATTCAGGCCATTACCGGGCGCAGGCGGGTGACGCTGATTGCAGAGGGGCGACGCTGTGAGCGCCTA  >  NC_000913/2769891‑2770161                                                                                                                                         |                                                                                                                                       cTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGcctc                                                                                                                                     <  2:1635899/140‑1 (MQ=255)  tGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTcc                                                                                                                                    <  2:1096055/140‑1 (MQ=255)  tGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGACCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTcc                                                                                                                                    <  2:1306156/140‑1 (MQ=255)   gCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTccc                                                                                                                                   >  1:1530864/1‑140 (MQ=255)    ccGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTTCCt                                                                                                                                  >  1:1241115/1‑140 (MQ=255)     cGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTg                                                                                                                                 >  1:516481/1‑140 (MQ=255)         tACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTg                                                                                                                             <  2:754111/140‑1 (MQ=255)          aCGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGACTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGa                                                                                                                            <  2:2970/140‑1 (MQ=255)              aCTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGc                                                                                                                        >  2:1365336/1‑140 (MQ=255)               ctgctgGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCa                                                                                                                       <  2:516481/140‑1 (MQ=255)                 gctgGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCAcc                                                                                                                     >  1:603376/1‑140 (MQ=255)                 gctgGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCAcc                                                                                                                     >  2:1458832/1‑140 (MQ=255)                  ctgGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCa                                                                                                                    >  1:346664/1‑140 (MQ=255)                  ctgGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCa                                                                                                                    >  2:1381011/1‑140 (MQ=255)                  ctgGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCa                                                                                                                    >  2:183546/1‑140 (MQ=255)                  ctgGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCa                                                                                                                    >  1:1130143/1‑140 (MQ=255)                  ctgGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGACGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCa                                                                                                                    >  2:1064044/1‑140 (MQ=255)                    ggCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGc                                                                                                                  <  2:603376/140‑1 (MQ=255)                    ggCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGc                                                                                                                  >  2:388417/1‑140 (MQ=255)                    ggCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGACGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGc                                                                                                                  <  1:1064044/140‑1 (MQ=255)                          gagaTGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAgt                                                                                                                  >  2:609666/1‑133 (MQ=255)                          gagaTGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAgt                                                                                                                  <  1:609666/134‑2 (MQ=255)                          gagaTGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGc                                                                                                                  >  2:859799/1‑134 (MQ=255)                          gagaTGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGc                                                                                                                  <  1:859799/134‑1 (MQ=255)                          gagaTGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATcc                                                                                                            >  1:835571/1‑140 (MQ=255)                          gagaTGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATcc                                                                                                            <  1:1365336/140‑1 (MQ=255)                            gaTGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCgg                                                                                                          >  2:279627/1‑140 (MQ=255)                               gCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTc                                                                                                       >  2:592737/1‑140 (MQ=255)                                 aGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCt                                                                                                     <  1:592737/140‑1 (MQ=255)                                 aGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCt                                                                                                     <  2:1130143/140‑1 (MQ=255)                                  gATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGc                                                                                                                  >  1:1058582/1‑126 (MQ=255)                                  gATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGc                                                                                                                  <  2:1058582/126‑1 (MQ=255)                                  gATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCtt                                                                                                    <  2:872326/140‑1 (MQ=255)                                     ggTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGc                                                                                                 >  1:720020/1‑140 (MQ=255)                                       tGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCt                                                                                               >  2:559013/1‑140 (MQ=255)                                       tGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCt                                                                                               >  1:1587956/1‑140 (MQ=255)                                       tGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCt                                                                                               >  2:384948/1‑140 (MQ=255)                                        gCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTc                                                                                                   <  2:1523360/135‑1 (MQ=255)                                        gCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTc                                                                                                   >  1:1523360/1‑135 (MQ=255)                                          ccGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCt                                                                                                     <  1:761102/131‑1 (MQ=255)                                          ccGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCt                                                                                                     >  2:761102/1‑131 (MQ=255)                                            gACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAgg                                                                                          <  1:279627/140‑1 (MQ=255)                                            gACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAgg                                                                                          <  1:1392195/140‑1 (MQ=255)                                             aCGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAg                                                                                           >  2:911868/1‑138 (MQ=255)                                             aCGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAg                                                                                           <  1:911868/138‑1 (MQ=255)                                             aCGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGa                                                                                         >  2:1646738/1‑140 (MQ=255)                                              cGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAg                                                                                        <  1:559013/140‑1 (MQ=255)                                              cGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAg                                                                                        <  1:384948/140‑1 (MQ=255)                                                 gCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCt                                                                                                     >  1:1564325/1‑124 (MQ=255)                                                 gCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCt                                                                                                     <  2:1564325/124‑1 (MQ=255)                                                 gCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCAt                                                                                     <  2:1219334/140‑1 (MQ=255)                                                 gCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCAt                                                                                     <  2:1587956/140‑1 (MQ=255)                                                 gCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCAt                                                                                     >  2:1368471/1‑140 (MQ=255)                                                 gCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCAt                                                                                     <  2:631195/140‑1 (MQ=255)                                                 gCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCAt                                                                                     <  2:346664/140‑1 (MQ=255)                                                   ttATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTg                                                                                   <  1:1368471/140‑1 (MQ=255)                                                     aTCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTTCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTc                                                                                 <  2:1241115/140‑1 (MQ=255)                                                          tGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCt                                                                            <  2:1530864/140‑1 (MQ=255)                                                               cccGCAACGGGCTGGCCGGGCTTTTTCCCGGCCTTGCCCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGgaga                                                                       <  1:333561/139‑1 (MQ=255)                                                               aCCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGgaga                                                                       >  2:1496086/1‑140 (MQ=255)                                                                ccGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGAc                                                                      <  1:1381011/140‑1 (MQ=255)                                                                ccGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGAc                                                                      <  1:183546/140‑1 (MQ=255)                                                                 cGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACt                                                                     >  1:330246/1‑140 (MQ=255)                                                                   cAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGa                                                                   >  2:995004/1‑140 (MQ=255)                                                                     gCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGAtt                                                                 <  1:1190970/140‑1 (MQ=255)                                                                     gCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGAtt                                                                 <  2:720020/140‑1 (MQ=255)                                                                         gCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGATTCAgg                                                             <  1:1496086/140‑1 (MQ=255)                                                                           tGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGATCCAGGcc                                                           >  2:518505/1‑140 (MQ=255)                                                                                  gCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGATTCAGGCCATTACCg                                                    >  1:653100/1‑140 (MQ=255)                                                                                    tttttCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGATTCAGGCCATTACCggg                                                  <  1:1123894/140‑1 (MQ=255)                                                                                      tttCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGATTCAGGCCATTACCGGgcg                                                <  1:1646738/140‑1 (MQ=255)                                                                                         cccGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGTTGAGCCTCCCTGCGTGATATCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGATTCAGGCCATTACCGGGCGCAg                                             <  2:1107795/140‑1 (MQ=255)                                                                                           cGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGATTCAGGCCATTAc                                                      >  2:1632996/1‑129 (MQ=255)                                                                                           cGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGATTCAGGCCATTAc                                                      <  1:1632996/129‑1 (MQ=255)                                                                                               cTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGATTCAGGCCATTACCGGGCGCAGGCGGGt                                       <  2:835571/140‑1 (MQ=255)                                                                                                  gACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGATTCAGGCCATTACCGGGCGCAGGCGGGTGAc                                    >  2:1580161/1‑140 (MQ=255)                                                                                                   aCCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGATTCAGGCCATTACCGGGCGCAGGCGGGTGACg                                   >  1:748084/1‑140 (MQ=255)                                                                                                   aCCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGATTCAGGCCATTACCGGGCGCAGGCGGGTGACg                                   <  2:653100/140‑1 (MQ=255)                                                                                                                       ctgctgATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGATTCAGGCCATTACCGGGCGCAGGCGGGTGACGCTGATTGCAGAGGGGCGACg               <  1:1580161/140‑1 (MQ=255)                                                                                                                        tgctgATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGATTCAGGCCATTACCGGGCGCAGGCGGGTGACGCTGATTGCAGAgggg                    <  1:512710/134‑1 (MQ=255)                                                                                                                        tgctgATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGATTCAGGCCATTACCGGGCGCAGGCGGGTGACGCTGATTGCAGAgggg                    >  2:512710/1‑134 (MQ=255)                                                                                                                         gctgATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGATTCAGGCCATTACCGGGCGCAGGCGGGTGACGCTGACTGCAGAGGGGc                   >  1:1350499/1‑134 (MQ=255)                                                                                                                         gctgATGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCCTTCTGGAGACTGATTCAGGCCATTACCGGGCGCAGGCGGGTGACGCTGATTGCAGAGGGGc                   <  2:1350499/134‑1 (MQ=255)                                                                                                                             aTGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGATTCAGGCCATTACCGGGCGCAGGCGGGTGACGCTGATTGCAGAGGGGCGACGCTGTGa         >  1:899224/1‑140 (MQ=255)                                                                                                                             aTGTGCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGATTCAGGCCATTACCGGGCGCAGGCGGGTGACGCTGATTGCAGAGGGGCGACGCTGTGa         <  2:330246/140‑1 (MQ=255)                                                                                                                               gtgCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGATTCAGGCCATTACCGGGCGCAGGCGGGTGACGCTGATTGCAGAGGGGCGACGCTGTGAgc       <  2:1113664/140‑1 (MQ=255)                                                                                                                               gtgCGATGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGATTCAGGCCATTACCGGGCGCAGGCGGGTGACGCTGATTGCAGAGGGGCGACGCTGTGAgc       <  1:1178653/140‑1 (MQ=255)                                                                                                                                    aTGAGCCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGATTCAGGCCATTACCGGGCGCAGGCGGGTGACGCTGATTGCAGAGGGGCGACGCTGTGAGCGCCTa  >  2:1152036/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CTGCCGCTTACGCACTGCTGGCAGTGAGATGCAGATGGTGCCCGACGTGCTTATCTTTGCCCACCGCAGCGGGCTGGCCGGGCTTTTTCCCGGCCTTGACCGCCGTCTGGTGAATGCACTGCTGATGTGCGATGAGTCTCCCTGCGTGATAGCACCAGCCAATCCGGTTCCTTCGCCTTCAGGAGCATTGTCTTTCTGGAGACTGATTCAGGCCATTACCGGGCGCAGGCGGGTGACGCTGATTGCAGAGGGGCGACGCTGTGAGCGCCTA  >  NC_000913/2769891‑2770161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑