Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,798,184	A→G	G104G (GGT→GGC)	stpA ←	DNA‑binding transcriptional repressor StpA with RNA chaperone activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,798,184	0	A	G	100.0%	257.1 / NA	72	G104G (GGT→GGC)	stpA	DNA‑binding transcriptional repressor StpA with RNA chaperone activity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/37);  total (35/37)

CGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATAACTCTTCCGGGTTAATTCCGTCAGCTTTCATCAGCTCCAGCCAGG  >  NC_000913/2798049‑2798317                                                                                                                                        |                                                                                                                                      cGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGt                                                                                                                                   >  1:39108/1‑140 (MQ=255) cGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGt                                                                                                                                   >  1:1514121/1‑140 (MQ=255) cGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGt                                                                                                                                   <  2:39108/140‑1 (MQ=255)      ccGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACa                                                                                                                              <  1:730302/140‑1 (MQ=255)         gACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCg                                                                                                                           <  2:1514121/140‑1 (MQ=255)          aCGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCgg                                                                                                                          <  2:255035/140‑1 (MQ=255)          aCGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCgg                                                                                                                          <  1:1349261/140‑1 (MQ=255)          aCGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCgg                                                                                                                          <  1:1442228/140‑1 (MQ=255)           cgcgCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGt                                                                                                                         <  1:696241/140‑1 (MQ=255)           cgcgCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGt                                                                                                                         <  2:605872/140‑1 (MQ=255)                         gACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCg                                                                                                           <  2:648474/140‑1 (MQ=255)                          aCATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGc                                                                                                          >  1:571365/1‑140 (MQ=255)                          aCATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGc                                                                                                          >  1:863448/1‑140 (MQ=255)                          aCATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGc                                                                                                          >  1:1528725/1‑140 (MQ=255)                          aCATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGc                                                                                                          >  1:369892/1‑140 (MQ=255)                              ccGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGa                                                                                                      <  1:1325482/140‑1 (MQ=255)                                   cTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCgg                                                                                                 <  1:1094711/140‑1 (MQ=255)                                      aGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTg                                                                                              <  1:1013432/140‑1 (MQ=255)                                      aGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTg                                                                                              >  1:528136/1‑140 (MQ=255)                                       gTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTgg                                                                                             <  2:571365/140‑1 (MQ=255)                                       gTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTgg                                                                                             <  1:641374/140‑1 (MQ=255)                                         aaTTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCg                                                                                           <  2:38523/140‑1 (MQ=255)                                               aTCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGtttttt                                                                                     >  2:1500736/1‑140 (MQ=255)                                                tCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTa                                                                                    >  2:1506641/1‑140 (MQ=255)                                                tCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTa                                                                                    >  1:177641/1‑140 (MQ=255)                                                tCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTa                                                                                    >  2:1541281/1‑140 (MQ=255)                                                 cAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTAc                                                                                   <  1:1541281/140‑1 (MQ=255)                                                  aGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTAcc                                                                                  >  2:849538/1‑140 (MQ=255)                                                  aGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTAcc                                                                                  >  1:1548431/1‑140 (MQ=255)                                                  aGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTAcc                                                                                  >  1:467146/1‑140 (MQ=255)                                                   ggAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCa                                                                                 <  1:1368960/140‑1 (MQ=255)                                                    gAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAg                                                                                >  1:286634/1‑140 (MQ=255)                                                    gAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAg                                                                                >  1:45692/1‑140 (MQ=255)                                                    gAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAg                                                                                >  1:1454556/1‑140 (MQ=255)                                                    gAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAg                                                                                >  1:1357852/1‑140 (MQ=255)                                                        tcgtcgAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGcgcg                                                                            <  1:1500736/140‑1 (MQ=255)                                                        tcgtcgAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGcgcg                                                                            <  2:1528725/140‑1 (MQ=255)                                                        tcgtcgAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGcgcg                                                                            <  2:323262/140‑1 (MQ=255)                                                        tcgtcgAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGcgcg                                                                            <  2:45692/140‑1 (MQ=255)                                                         cgtcgAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGCCCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCgt                                                                           <  2:1454556/140‑1 (MQ=255)                                                         cgtcgAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCgt                                                                           <  2:1548431/140‑1 (MQ=255)                                                         cgtcgAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCgt                                                                           <  2:931209/140‑1 (MQ=255)                                                         cgtcgAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCgt                                                                           <  2:99972/140‑1 (MQ=255)                                                         cgtcgAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCgt                                                                           <  1:1354211/140‑1 (MQ=255)                                                             gagagaTTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCgtggtg                                                                       <  1:1630857/140‑1 (MQ=255)                                                                  aTTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTgcagca                                                                  >  1:1658160/1‑140 (MQ=255)                                                                    ttACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTgcagcagc                                                                <  1:1506641/140‑1 (MQ=255)                                                                    ttACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTgcagcagc                                                                <  2:369892/140‑1 (MQ=255)                                                                      aCCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGcagcagcag                                                              <  1:1651757/140‑1 (MQ=255)                                                                          tCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCt                                                          >  2:57913/1‑140 (MQ=255)                                                                           cTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTa                                                         >  1:1152712/1‑140 (MQ=255)                                                                            tGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGcagcagcag                                                              <  2:888663/134‑1 (MQ=255)                                                                            tGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGcagcagcag                                                              >  1:888663/1‑134 (MQ=255)                                                                             gCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCG‑TTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTa                                                      <  2:1221618/140‑1 (MQ=255)                                                                                 gcgcCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTAccc                                                   <  1:849538/140‑1 (MQ=255)                                                                                    ccTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAAt                                                >  1:1519938/1‑140 (MQ=255)                                                                                     cTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATa                                               <  2:1579946/140‑1 (MQ=255)                                                                                            aTTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATAACTCTTc                                        >  1:1051961/1‑140 (MQ=255)                                                                                            aTTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATAACTCTTc                                        <  2:1357852/140‑1 (MQ=255)                                                                                             ttGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATAACTCTTcc                                       >  1:447216/1‑140 (MQ=255)                                                                                             ttGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATAACTCTTcc                                       >  1:668983/1‑140 (MQ=255)                                                                                                    cGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATAACTCTTCCGGGTTaa                                >  1:44363/1‑140 (MQ=255)                                                                                                     ggTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATAACTCTTCCGGGTTAAt                               <  2:1519938/140‑1 (MQ=255)                                                                                                                cTGACCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATAACTCTTCCGGGTTAATTCCGTCAGCtt                    >  2:958187/1‑140 (MQ=255)                                                                                                                   aCCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATAACTCTTCCGGGTTAATTCCGTCAGCTTtca                 >  1:949950/1‑140 (MQ=255)                                                                                                                   aCCGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATAACTCTTCCGGGTTAATTCCGTCAGCTTtca                 >  2:40292/1‑140 (MQ=255)                                                                                                                    ccGGTCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATAACTCTTCCGGGTTAATTCCGTCAGCTTtcat                <  1:463550/140‑1 (MQ=255)                                                                                                                        tCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATAACTCTTCCGGGTTAATTCCGTCAGCTTTCATCAGc            >  1:805892/1‑140 (MQ=255)                                                                                                                        tCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATAACTCTTCCGGGTTAATTCCGTCAGCTTTCATCAGc            >  2:1529595/1‑140 (MQ=255)                                                                                                                        tCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATAACTCTTCCGGGTTAATTCCGTCAGCTTTCATCAGc            >  1:1166816/1‑140 (MQ=255)                                                                                                                        tCCAGGTTTTAGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATAACTCTTCCGGGTTAATTCCGTCAGCTTTCATCAGc            >  2:994970/1‑140 (MQ=255)                                                                                                                                  aGTTTCGCCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATAACTCTTCCGGGTTAATTCCGTCAGCTTTCATCAGCTCCAGCCAgg  >  2:557219/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      CGACGCCGGACGCGCCCTAGCAGCGACATCCGGCCTCAGTAATTAGATCAGGAAATCGTCGAGAGATTTACCTTCTGCCAGCGCCTGAGCAATTGGCTTCGGTGTACGGCCCTGACCGGTCCAGGTTTTAGTTTCACCGTTAACATCGGTGAATTTATATTTCGCCGGACGCGGCTGGCGTTTTTTACCAGCGCGTGGTGCAGCAGCAGAGCTATTACCCAATAACTCTTCCGGGTTAATTCCGTCAGCTTTCATCAGCTCCAGCCAGG  >  NC_000913/2798049‑2798317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑