Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,947,343	(A)8→7	intergenic (‑165/‑44)	mltA ← / → metZ	membrane‑bound lytic murein transglycosylase A/tRNA‑Met

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,947,336	0	A	.	100.0%	220.8 / NA	52	intergenic (‑158/‑51)	mltA/metZ	membrane‑bound lytic murein transglycosylase A/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (17/35);  total (17/35)

AGGCGCAACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCA  >  NC_000913/2947205‑2947459                                                                                                                                    |                                                                                                                            aGGCGCAACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTaaaaaaagg                                                                                                                     <  2:947870/140‑3 (MQ=255) aGGCGCAACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTaaaaaaagg                                                                                                                     <  1:452324/140‑3 (MQ=255) aGGCGCAACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTaaaaaaagg                                                                                                                     <  2:19766/140‑3 (MQ=255)       aaCGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCAt                                                                                                              >  1:1254655/1‑140 (MQ=255)          gAAGATAACAATCCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGaa                                                                                                           <  1:673867/140‑1 (MQ=255)             gATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAAc                                                                                                        >  2:712339/1‑140 (MQ=255)              aTAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCTCAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAAcg                                                                                                       >  1:408421/1‑140 (MQ=255)               tAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCTCAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAAcgc                                                                                                      <  2:408421/140‑1 (MQ=255)               tAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAAcgc                                                                                                      <  2:736794/140‑1 (MQ=255)               tAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAAcgc                                                                                                      <  2:1372512/140‑1 (MQ=255)               tAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAAcgc                                                                                                      <  1:684709/140‑1 (MQ=255)                 aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                    <  1:1152849/140‑1 (MQ=255)                 aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                    >  2:628650/1‑140 (MQ=255)                 aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                    <  2:481465/140‑1 (MQ=255)                 aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                    <  2:208496/140‑1 (MQ=255)                 aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                    <  2:1228521/140‑1 (MQ=255)                 aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                    <  1:280661/140‑1 (MQ=255)                 aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                    <  2:785505/140‑1 (MQ=255)                 aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                    <  1:490117/140‑1 (MQ=255)                 aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTG‑‑‑GAAAACGCGAGCg                                                                                                 <  1:836301/140‑1 (MQ=255)                  cAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAg                                                                                                   <  1:1094991/140‑1 (MQ=255)                    aaCCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCg                                                                                                 <  2:801367/140‑1 (MQ=255)                    aaCCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCg                                                                                                 <  1:159917/140‑1 (MQ=255)                        gccgcGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGt                                                                                             >  1:248017/1‑140 (MQ=255)                         ccgcGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGta                                                                                            <  2:137997/140‑1 (MQ=255)                         ccgcGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGta                                                                                            >  1:655676/1‑140 (MQ=255)                         ccgcGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGta                                                                                            <  2:1254655/140‑1 (MQ=255)                                      aatgaGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCa                                                                               <  1:918392/140‑1 (MQ=255)                                            cagcCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGAcg                                                                         >  1:10353/1‑140 (MQ=255)                                            cagcCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGAcg                                                                         <  2:10353/140‑1 (MQ=255)                                            cagcCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGAcg                                                                         <  1:1172845/140‑1 (MQ=255)                                              gcCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGAcgcg                                                                       <  1:1327105/140‑1 (MQ=255)                                                 tctcCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCgggg                                                                    <  1:1343323/140‑1 (MQ=255)                                                  ctcCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGt                                                                   >  1:1196056/1‑140 (MQ=255)                                                   tcCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTg                                                                  <  1:1270171/140‑1 (MQ=255)                                                    cccTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTgg                                                                 >  2:686638/1‑140 (MQ=255)                                                      cTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAATGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGag                                                               <  2:762228/140‑1 (MQ=255)                                                      cTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGag                                                               <  2:655676/140‑1 (MQ=255)                                                      cTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGag                                                               <  2:657684/140‑1 (MQ=255)                                                      cTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGag                                                               <  1:771255/140‑1 (MQ=255)                                                        gACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGagca                                                             <  2:1337490/140‑1 (MQ=255)                                                         aCGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGagcag                                                            >  2:1132125/1‑140 (MQ=255)                                                                       caAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCtcgtc                                              <  2:1367609/140‑1 (MQ=255)                                                                                  cTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCGTGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAAc                                   >  1:1076337/1‑140 (MQ=255)                                                                                  cTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAAc                                   >  2:330396/1‑140 (MQ=255)                                                                                   tttAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAAcc                                  <  1:823237/140‑1 (MQ=255)                                                                                    ttAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAAccc                                 >  1:1131286/1‑140 (MQ=255)                                                                                           tttgtttttgttCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtc                          >  2:1058791/1‑140 (MQ=255)                                                                                            ttgtttttgttCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcg                         >  1:140701/1‑140 (MQ=255)                                                                                            ttgtttttgttCAAAATCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcg                         >  1:62501/1‑140 (MQ=255)                                                                                                        aaaaTCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATc             <  1:686638/140‑1 (MQ=255)                                                                                                          aaTCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCg           <  2:1196056/140‑1 (MQ=255)                                                                                                               tGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGcccc      >  2:1302610/1‑140 (MQ=255)                                                                                                                 ccAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCg    <  1:712339/140‑1 (MQ=255)                                                                                                                   aaaTCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCa  <  1:1034793/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                            AGGCGCAACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCA  >  NC_000913/2947205‑2947459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑