Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,240,984	(G)7→8	coding (1041/1245 nt)	sstT →	serine/threonine:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,240,977	1	.	G	100.0%	79.2 / NA	24	A345G (GCG→GGG)	sstT	serine/threonine:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (3/21);  total (3/21)

TGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑GG‑GGGGGTCT‑CTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAAC  >  NC_000913/3240842‑3241107                                                                                                                                         |                                                                                                                                      tGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑gg‑gg                                                                                                                                 <  2:650344/140‑1 (MQ=255)     gggcgCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑gg‑gggggg                                                                                                                             <  1:721565/140‑2 (MQ=255)      ggcgCAGCAATCACTATTCCCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑gg‑ggggggt                                                                                                                            <  2:186571/140‑3 (MQ=255)      ggcgCAGCAATCACTATTACCGTTTTGACGCTGGCTGCGGGTAATACGCTGGGTATTCCGGTCGATCTCCCCCCGCCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑gg‑ggggggt                                                                                                                            <  2:1260816/140‑3 (MQ=255)           agcaATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGTCT‑ctg                                                                                                                       <  1:496818/140‑1 (MQ=255)                       tcccGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGTCT‑CTGCTGCTGATCCCa                                                                                                           <  1:65265/138‑1 (MQ=255)                       tACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGTCT‑CTGCTGCTGATCCCa                                                                                                           <  2:164563/140‑1 (MQ=255)                       tACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGTCT‑CTGCTGCTGATCCCa                                                                                                           <  1:844315/140‑1 (MQ=255)                       tACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGTCT‑CTGCTGCTGATCCCa                                                                                                           <  2:3666/140‑1 (MQ=255)                       tACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGTCT‑CTGCTGCTGATCCCa                                                                                                           <  1:712246/140‑1 (MQ=255)                         ccGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGTCT‑CTGCTGCTGATCCCACt                                                                                                         <  1:1382190/140‑1 (MQ=255)                         ccGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGGCT‑CTGCTTCTGGTcccccc                                                                                                         >  2:659055/1‑137 (MQ=255)                          cGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGTCT‑CTGCTGCTGATCCCACTg                                                                                                        <  1:1323570/140‑1 (MQ=255)                            tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGGGGGT‑CTGCTGCTGATcccccccg                                                                                                       >  1:1064849/1‑135 (MQ=255)                            tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGTCT‑CTGCTGCTGATCCCACTGGc                                                                                                      <  1:564292/140‑1 (MQ=255)                            tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGTCT‑CTGCTGCTGATCCCACTGGc                                                                                                      >  2:479820/1‑140 (MQ=255)                            tgtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGTCT‑CTGCTGCTGATCCCACTGGc                                                                                                      <  1:214486/140‑1 (MQ=255)                              ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGTCT‑CTGCTGCTGATCCCACTGGCCt                                                                                                    >  1:746738/1‑140 (MQ=255)                              ttGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGGCT‑CTGCTGCTGATCCCACTGGccc                                                                                                    >  1:755171/1‑139 (MQ=255)                                 aCGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGTCT‑CTGCTGCTGATCCCACTGGCCTGTa                                                                                                 <  1:1280820/140‑1 (MQ=255)                                 aCGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGGCT‑CTGCTGCTGATCCCCCCTGCCTGTa                                                                                                 >  2:573441/1‑140 (MQ=255)                                   gctggctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGTCT‑CTGCTGCTGATCCCACTGGCCTGTAat                                                                                               <  1:659055/140‑1 (MQ=255)                                      ggctgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGGCT‑CTGCTGCTGATCCCCCCGGCCTGTAatatt                                                                                            >  2:979517/1‑139 (MQ=255)                                           cGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGGCT‑CTGCTGCTGATCCCCCCGGCCTGTAATATGTTTgg                                                                                       >  2:296242/1‑140 (MQ=255)                                                    cGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGTCT‑CTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGaa                                                                              <  1:979517/140‑1 (MQ=255)                                                      cTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGGGTCT‑CTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAAc                                                                             >  1:960617/1‑140 (MQ=255)                                                      cTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGGCT‑CTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGGATTTCGAACg                                                                            >  1:81435/1‑140 (MQ=255)                                                       tGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGGCT‑CTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGa                                                                           >  2:483883/1‑140 (MQ=255)                                                        gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGGCT‑CTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGat                                                                          >  1:962375/1‑140 (MQ=255)                                                        gggTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGGCT‑CTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGat                                                                          >  1:87128/1‑140 (MQ=255)                                                          gTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGTCT‑CTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGatat                                                                        <  1:745894/140‑1 (MQ=255)                                                                   tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGGCT‑TTGCTGCTGATCCCCCCGGGCTGTAATATGTTTGGGGTTTTGGAGGGTATCGGCATggc                                                               >  2:1395313/1‑138 (MQ=255)                                                                   tCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑GGTTGGGGGGT‑CTAGTGCGGATTCCTCTTGCCTTTGATATATTTGGTACTTCGGTCGCTATCTcccatac                                                               >  2:694897/1‑135 (MQ=255)                                                                               cGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGTCT‑CTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGt                                                   <  2:87128/140‑1 (MQ=255)                                                                                ggCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGGCT‑CTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGGATTTCGAACGATATCGCCCTGCAGGGGGGTGGCggc                                                  >  2:518007/1‑138 (MQ=255)                                                                                     tgctgTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑GG‑GGGGGGGTCCTGCTGCTTATCCCCCCGGCCCGGAATATGTTCGGGATTTCGCACGATATTGCCACGCAGGGGGGTGGCGGCGGCtt                                             >  2:1354859/1‑140 (MQ=255)                                                                                               cGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGGCT‑CTGCTGCTGATCCCACTGGCCCGTAATATGTTTGGGATTTTGAACGATATCGCCCTGCAGGGGGGTGCCGTCGGCTTTTTCATCGGc                                   >  2:678085/1‑140 (MQ=255)                                                                                                 tggtggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGGCT‑CTGCTGCTGATCCCCCCGGCCTGTAATATTTTCGGGATTTTGAACGATATCGCCCTGCAGGGGGGTGCCGGCGGCTTTTTTATCGGCgg                                 >  2:1303465/1‑139 (MQ=255)                                                                                                 tggtggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGGCT‑CTGCTGCTGATCCCCCCGGCCTGTAATATGTTCGGGATTTTGAACGATATCGCCCTGCAGGTGGTTGCCGTCGGCTTTTTCATCcgcgg                                 >  2:1157538/1‑139 (MQ=255)                                                                                                 tggtggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGGCT‑CTGCTGCTGATCCCACTGGCCTGTAATATGTTTGGTATTTCGGACGGGATCGCCACGCAGGGGGGTGCCGTCGGCTTTATCATCGGCgg                                 >  2:170338/1‑139 (MQ=255)                                                                                                        ttCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGTCT‑CTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAg                          <  2:841521/140‑1 (MQ=255)                                                                                                          ctctGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGTCT‑CTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGa                        <  1:483883/140‑1 (MQ=255)                                                                                                           tctGTGTGCCTGTGGCGCATCCGGCGTGGCG‑GG‑GGGGGTCT‑CTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGAc                       <  2:703134/140‑1 (MQ=255)                                                                                                                     tgtgGCGCATCCGGCGTGGCG‑GG‑GGGGGGCT‑CTGCTGCTGATCCCCCCGGCCCGTAAAATGTTCGGGATTTTGAACGACATCGCCCTGCCGGTGGGTGCCCTCGGCCTTATTATCGGCGGATTGCAGGACTCTTGCGaaa             >  2:175904/1‑140 (MQ=255)                                                                                                                      gtgGCGCATCCGGCGTGGCGGGG‑GGGGGTCT‑CTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGaaa             <  2:960617/140‑1 (MQ=255)                                                                                                                               ccGGCGTGGCG‑GG‑GGGGGTCT‑CTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGaa   <  1:479820/140‑1 (MQ=255)                                                                                                                                cGGCGTGGCG‑GG‑GGGGGTCT‑CTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAAc  <  1:1157538/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      TGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGC‑‑GG‑GGGGGTCT‑CTGCTGCTGATCCCACTGGCCTGTAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAAC  >  NC_000913/3240842‑3241107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑