Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,180,388	+G	intergenic (+29/+91)	cobB → / ← ycfZ	protein‑lysine deacetylase/desuccinylase/putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,180,384	1	.	G	100.0%	116.0 / NA	34	intergenic (+25/+95)	cobB/ycfZ	protein‑lysine deacetylase/desuccinylase/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (20/14);  total (20/14)

AGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAA‑GGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCATCCGGAATAATACACGCGCACTTTTCCGGCCTCTTTTATCGTTTACTCGTCAATAACAATGCGGCAATAAACGTA  >  NC_000913/1180249‑1180518                                                                                                                                          |                                                                                                                                       aGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAA‑gggg                                                                                                                                    >  1:1213289/1‑140 (MQ=255) aGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAA‑gggg                                                                                                                                    >  1:60346/1‑140 (MQ=255)  gTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAA‑ggggg                                                                                                                                   <  1:1073695/140‑2 (MQ=255)           gTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAAGGGGGACGCaatta                                                                                                                           >  2:105809/1‑140 (MQ=255)            tAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAA‑g                                                                                                                                       >  2:772211/1‑126 (MQ=255)            tAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAA‑g                                                                                                                                       <  1:772211/126‑1 (MQ=255)            tAATGAATTCGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAAGGGGGACGCaattaa                                                                                                                          >  1:421325/1‑140 (MQ=255)               tGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTg                                                                                                                       <  1:978218/140‑1 (MQ=255)                     tGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTg                                                                                                                 <  1:105809/140‑1 (MQ=255)                             aaTATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGtttt                                                                                                         >  1:1342233/1‑140 (MQ=255)                               tatTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTtgt                                                                                                       <  2:421325/140‑1 (MQ=255)                                 ttACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAAGGGGGACGCGATTAATTGAATTTGCGGGTTTTGTgg                                                                                                     >  1:44861/1‑140 (MQ=255)                                  tACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTggg                                                                                                    <  2:1342233/140‑1 (MQ=255)                                   aCGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGc                                                                                                   <  2:60346/140‑1 (MQ=255)                                   aCGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGc                                                                                                   <  1:387689/140‑1 (MQ=255)                                      gCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCgg                                                                                                >  1:668562/1‑140 (MQ=255)                                       cccGGCAAGCCAGGTGGTGCCTGAGGTTGTTGGAAAGTTGCTGGAGGGATTAAAAACGGGAAGCATTGCCTGATGCACTACGCTTATCCGGCCTGCCAG‑GGGGGCGCAATTAATTGAAATTGCGGGGTTTGTGGGGCggg                                                                                               >  2:1064563/1‑139 (MQ=255)                                         cGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATa                                                                                             >  1:112492/1‑140 (MQ=255)                                                          ccTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCAt                                                                            >  2:258643/1‑140 (MQ=255)                                                           cTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCATc                                                                           <  1:1074582/140‑1 (MQ=255)                                                                        aaaGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCATCCGGAATAATacac                                                              <  1:240861/140‑1 (MQ=255)                                                                                     ggATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCATCCGGAATAATACACGCGCACTTTTCCg                                                 <  2:1213289/140‑1 (MQ=255)                                                                                      gATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCATCCGGAATAATACACGCGCACTTTTCCgg                                                >  1:821549/1‑140 (MQ=255)                                                                                            aaGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCATCCGGAATAATACACGCGCACTTTTCCGGCCTCtt                                          <  1:1064563/140‑1 (MQ=255)                                                                                                 ggAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCATCCGGAATAATACACGCGCACTTTTCCGGCCTCTTTTATc                                     >  1:264440/1‑140 (MQ=255)                                                                                                        ttGCCTGATGCACTACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCATCCGGAATAATACACGCGCACTTTTCCGGCCTCTTTTATCGTTTACt                              >  2:784865/1‑140 (MQ=255)                                                                                                                tGCACTACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCATCCGGAATAATACACGCGCACTTTTCCGGCCTCTTTTATCGTTTAc                               <  1:1397147/131‑1 (MQ=255)                                                                                                                tGCACTACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCATCCGGAATAATACACGCGCACTTTTCCGGCCTCTTTTATCGTTTAc                               >  2:1397147/1‑131 (MQ=255)                                                                                                                tGCACTACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCATCCGGAATAATACACGCGCACTTTTCCGGCCTCTTTTATCGTTTACTCGTCAATa                      >  1:1025008/1‑140 (MQ=255)                                                                                                                    cTACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCATCCGGAATAATACACGCGCACTTTTCCGGCCTCTTTTATCGTTTACTCGTCAATAACaa                  >  2:817381/1‑140 (MQ=255)                                                                                                                     tACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCATCCGGAATAATACACGCGCACTTTTCCGGCCTCTTTTATCGTTTACTc                             <  1:1240948/128‑1 (MQ=255)                                                                                                                     tACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCATCCGGAATAATACACGCGCACTTTTCCGGCCTCTTTTATCGTTTACTc                             >  2:1240948/1‑128 (MQ=255)                                                                                                                     tACGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCATCCGGAATAATACACGCGCACTTTTCCGGCCTCTTTTATCGTTTACTCGTCAATAACAAt                 >  2:117444/1‑140 (MQ=255)                                                                                                                       cGCTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCATCCGGAATAATACACGCGCACTTTTCCGGCCTCTTTTATCGTTTACTCGTCAATAACAATGc               >  1:372246/1‑140 (MQ=255)                                                                                                                         cTTATCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCATCCGGAATAATACACGCGCACTTTTCCGGCCTCTTTTATCGTTTACTCGTCAATAACAATGCgg             >  2:814623/1‑140 (MQ=255)                                                                                                                            aTCAGGCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCATCCGGAATAATACACGCGCACTTTTCCGGCCTCTTTTATCGTTTACTCGTCAATAACAATGCGGCaa          <  1:817381/140‑1 (MQ=255)                                                                                                                                 gCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCATCCGGAATAATACACGCGCACTTTTCCGGCCTCTTTTATCGTTTACTCGTCAATAACAATGCGGCAATAAAc     >  1:351266/1‑140 (MQ=255)                                                                                                                                 gCCTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCATCCGGAATAATACACGCGCACTTTTCCGGCCTCTTTTATCGTTTACTCGTCAATAACAATGCGGCAATAAAc     >  2:667260/1‑140 (MQ=255)                                                                                                                                  ccTG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCATCCGGAATAATACACGCGCACTTTTCCGGCCTCTTTTATCGTTTACTCGTCAATAACAATGCGGCAATAAACg    >  2:245205/1‑140 (MQ=255)                                                                                                                                    tG‑CAAGGGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCATCCGGAATAATACACGCGCACTTTTCCGGCCTCTTTTATCGTTTACTCGTCAATAACAATGCGGCAATAAACGTa  <  1:258643/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       AGTCAGGTTGGTAATGAATTTGCCGAGAAATATTACGGCCCGGCAAGCCAGGTGGTGCCTGAGTTTGTTGAAAAGTTGCTGAAGGGATTAAAAGCGGGAAGCATTGCCTGATGCACTACGCTTATCAGGCCTG‑CAA‑GGGGACGCAATTAATTGAATTTGCGGGTTTTGTGGGCCGGATAAGGCGTTCGCGCCCCATCCGGAATAATACACGCGCACTTTTCCGGCCTCTTTTATCGTTTACTCGTCAATAACAATGCGGCAATAAACGTA  >  NC_000913/1180249‑1180518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑