| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,236,278 | (G)6→7 | coding (964/1533 nt) | ycgB ← | PF04293 family protein YcgB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,236,272 | 1 | . | G | 96.5% | 191.9 / ‑0.1 | 57 | coding (970/1533 nt) | ycgB | PF04293 family protein YcgB |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (22/33); total (23/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01 | |||||||||||
AGCCAGTCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATA‑‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTTCATCAC > NC_000913/1236136‑1236404 | agccagTCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATA‑‑ggg < 1:1393772/140‑1 (MQ=255) ggAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATA‑‑gggggggctgg < 2:735343/140‑6 (MQ=255) ggAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATA‑‑gggggggctgg < 2:344499/140‑6 (MQ=255) ggAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATA‑‑gggggggctgg < 1:299812/140‑6 (MQ=255) aaCCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGaa < 1:998093/140‑1 (MQ=255) aaCCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGaa > 1:1372859/1‑140 (MQ=255) ccGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGa < 2:572466/140‑1 (MQ=255) gCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACca > 1:1367828/1‑140 (MQ=255) cGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACcac < 1:1058682/140‑1 (MQ=255) cGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACcac < 1:539815/140‑1 (MQ=255) cGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACcac < 1:1409561/140‑1 (MQ=255) tatCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACAtt < 2:80610/140‑1 (MQ=255) cGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGgtg < 1:431122/140‑1 (MQ=255) ggAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGtgtg > 2:858737/1‑140 (MQ=255) aaCCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGc < 1:313270/140‑1 (MQ=255) gTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGc > 2:598972/1‑140 (MQ=255) tttGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCaaa < 2:747838/140‑1 (MQ=255) gTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAAc > 1:1379002/1‑140 (MQ=255) cttcttCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTc < 1:598972/140‑1 (MQ=255) cttcCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGGGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAg > 1:562483/1‑140 (MQ=255) cttcCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAg > 2:306629/1‑140 (MQ=255) cttcCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAg > 1:255252/1‑140 (MQ=255) cttcCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATA‑‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGc > 1:242828/1‑140 (MQ=255) ttcCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGc < 1:357814/140‑1 (MQ=255) ggCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACGCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAAc < 2:939498/140‑1 (MQ=255) ggCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAAc > 2:863962/1‑140 (MQ=255) gCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACg < 1:99199/140‑1 (MQ=255) gACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGtt < 2:911172/140‑1 (MQ=255) aCTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTc < 2:151922/140‑1 (MQ=255) aCTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTc < 2:1324805/140‑1 (MQ=255) cTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTcc < 2:1372859/140‑1 (MQ=255) tGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCg > 2:696465/1‑140 (MQ=255) ccGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATA‑‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTcc < 2:242828/140‑1 (MQ=255) cGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTcc < 1:858737/140‑1 (MQ=255) tttAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCt < 1:1318375/140‑1 (MQ=255) tttAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCt < 2:1379002/140‑1 (MQ=255) tttAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCt < 1:129598/140‑1 (MQ=255) tCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTA‑CACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTtcatca > 1:431866/1‑138 (MQ=255) tCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTA‑CACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTtcatca < 2:431866/138‑1 (MQ=255) tGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCt > 2:660785/1‑130 (MQ=255) tGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCt < 1:660785/130‑1 (MQ=255) tGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACa > 1:227116/1‑140 (MQ=255) tGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACa < 2:1130569/140‑1 (MQ=255) ggAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAg < 2:227116/140‑1 (MQ=255) gAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGa < 1:863962/140‑1 (MQ=255) tGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGtt > 1:1033185/1‑140 (MQ=255) tGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGtt > 2:96899/1‑140 (MQ=255) tGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGtt > 1:38298/1‑140 (MQ=255) tGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGtt < 1:696465/140‑1 (MQ=255) tGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGtt > 2:760808/1‑140 (MQ=255) tGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGtt < 2:255252/140‑1 (MQ=255) tGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGtt < 2:1184406/140‑1 (MQ=255) tGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGtt < 2:57827/140‑1 (MQ=255) tGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGtt > 1:1184406/1‑140 (MQ=255) gCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTaa > 1:619725/1‑140 (MQ=255) aaCCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGa < 2:619725/140‑1 (MQ=255) aGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGgtgt > 2:204806/1‑140 (MQ=255) cTGTACCACGGGCTGTTATAGGGGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAg > 2:1397734/1‑140 (MQ=255) gCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTtcatc > 1:808651/1‑140 (MQ=255) gCTGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTtcatc < 1:204806/140‑1 (MQ=255) tGTTATAG‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTTCATCAc < 2:616776/140‑1 (MQ=255) | AGCCAGTCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGTTATA‑‑GGGGGGCTGGAAGACCACATTGGTGTGGCTGTGCAAAAACTCCAGCATAAAACGTTCCGTTACTTTCCCTTCATCATACAGATGGTTAAGGATGGTGTAGTGCCAGAAGGTCGCCCAGCCTTCGTTCATCAC > NC_000913/1236136‑1236404 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |