| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,252,395 | (G)5→6 | coding (1330/1920 nt) | dhaR → | DNA‑binding transcriptional dual regulator DhaR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,252,390 | 1 | . | G | 97.8% | 153.8 / ‑3.2 | 45 | coding (1325/1920 nt) | dhaR | DNA‑binding transcriptional dual regulator DhaR |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (20/24); total (21/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01 | |||||||||||
TTTATTGGTGGCGATCGCACGGACAATGAAAATGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑A‑GGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCA > NC_000913/1252251‑1252522 | tttATTGGTGGCGATCGCACGGACAATGAAAATGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑a < 1:304952/140‑1 (MQ=255) ttATTGGTGGCGATCGCACGGACAATGAAAATGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAAGATTGAATATCTGGAGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑A‑g < 2:149641/140‑1 (MQ=255) tATTGGTGGCGATCGCACGGACAATGAAAATGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑A‑gg < 1:520354/140‑1 (MQ=255) aTTGGTGGCGATCGCACGGACAATGAAAATGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑A‑ggg < 1:1068053/140‑1 (MQ=255) gATCGCACGGACAATGAAAATGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCa < 2:1394943/140‑1 (MQ=255) cGCACGGACAATGAAAATGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCAcgc < 2:124023/140‑1 (MQ=255) aaaaTGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGcgcg < 2:518481/140‑1 (MQ=255) tGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGt > 2:1017254/1‑140 (MQ=255) tGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGt > 1:794358/1‑140 (MQ=255) ggCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGtt < 2:1019619/140‑1 (MQ=255) ggCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGtt < 2:683715/140‑1 (MQ=255) cTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATAc < 2:734773/140‑1 (MQ=255) gTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAAt < 2:794358/140‑1 (MQ=255) tCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAAtt < 1:65549/140‑1 (MQ=255) aCTGGCACACGGCGGCACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCaaa < 1:1317602/140‑1 (MQ=255) aCTGGCACACGGCGGCACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCaaa < 2:1112543/140‑1 (MQ=255) tGGCACACGGCGGCACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTCCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGt < 1:1068550/140‑1 (MQ=255) gCACACGGCGGCACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGa < 2:1188023/140‑1 (MQ=255) acacGGCGGCACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGAtt < 1:256961/140‑1 (MQ=255) gcACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCaacaac > 1:111478/1‑140 (MQ=255) gcACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCaacaac > 1:1296954/1‑140 (MQ=255) ttCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGa < 2:216036/140‑1 (MQ=255) tGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTc > 1:279225/1‑140 (MQ=255) aaaGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCa > 1:796978/1‑140 (MQ=255) aaaGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCa < 1:1274977/140‑1 (MQ=255) aTTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGc < 1:33358/140‑1 (MQ=255) aTTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGCTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGc > 2:392551/1‑140 (MQ=255) cTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAAc > 1:943106/1‑140 (MQ=255) tGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGCAA‑GGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACa > 2:1261933/1‑140 (MQ=255) ggCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACaa > 1:926425/1‑140 (MQ=255) tGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCg > 1:982697/1‑140 (MQ=255) gAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGtt > 2:1214853/1‑140 (MQ=255) gTCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGc > 1:1133068/1‑140 (MQ=255) tCTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGcc < 1:1356911/140‑1 (MQ=255) cTGCTTTGCTTCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCa > 1:371750/1‑140 (MQ=255) tgctttgcttCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAg > 2:985431/1‑140 (MQ=255) tgctttgcttCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAg > 2:905230/1‑140 (MQ=255) tgctttgcttCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAg < 2:279225/140‑1 (MQ=255) tgctttgcttCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAg < 2:1094295/140‑1 (MQ=255) tgctttgcttCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAg > 1:1378754/1‑140 (MQ=255) cttCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTAtt < 2:938578/140‑1 (MQ=255) ttCAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTa > 2:1237318/1‑140 (MQ=255) cAGGTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTAcg < 1:1017254/140‑1 (MQ=255) ggTTATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTAcgcg > 2:476355/1‑140 (MQ=255) tATCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCtg > 2:406818/1‑140 (MQ=255) tCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCtgca < 2:326825/140‑1 (MQ=255) tCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCtgca > 2:473903/1‑140 (MQ=255) tCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCtgca < 2:169459/140‑1 (MQ=255) tCAAGC‑AGGGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCtgca < 2:926425/140‑1 (MQ=255) | TTTATTGGTGGCGATCGCACGGACAATGAAAATGGCCGTCTGAGTCGGCTGGAACTGGCACACGGCGGCACGCTGTTTCTTGAAAAGATTGAATATCTGGCGGTGGAGTTACAGTCTGCTTTGCTTCAGGTTATCAAGC‑A‑GGGGGTTATCACGCGACTGGATGCGCGGCGTTTAATACCAATTGATGTCAAAGTGATTGCAACAACGACCGCGGACCTCGCAATGCTGGTGGAACAAAATCGTTTTAGTCGCCAGCTGTATTACGCGCTGCA > NC_000913/1252251‑1252522 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |