Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,131,407	G→A	I334I (ATC→ATT)	yghQ ←	putative transport protein YghQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,131,407	0	G	A	100.0%	92.7 / NA	27	I334I (ATC→ATT)	yghQ	putative transport protein YghQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/6);  total (21/6)

CGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAA  >  NC_000913/3131271‑3131539                                                                                                                                         |                                                                                                                                     cGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACAATc                                                                                                                                   >  1:844440/1‑140 (MQ=255)     tCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACAATCAaca                                                                                                                               <  1:1391017/140‑1 (MQ=255)            tGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACAATCAACACCGCGAg                                                                                                                        <  2:923783/140‑1 (MQ=255)            tGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACAATCAACACCGCGAg                                                                                                                        <  1:978359/140‑1 (MQ=255)                gaaatgaaatGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACAATCAACACCGCGAGCGcc                                                                                                                    <  1:646430/140‑1 (MQ=255)                  aatgaaatgaCTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCAACAATCAACACCGCGAGCGccac                                                                                                                  <  1:1092227/140‑1 (MQ=255)                               aGTCAGCCGATAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACAATCAACACCGCGAGCGCCACCAGAATACCGAtt                                                                                                     <  1:1316000/140‑1 (MQ=255)                                    gCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACAATCAACACCGCGAGCGCCACCAGAATACCGATTccgcc                                                                                                >  1:531913/1‑140 (MQ=255)                                          aagaaTTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACAATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAg                                                                                          >  1:1361380/1‑140 (MQ=255)                                          aagaaTTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACAATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAg                                                                                          >  1:592527/1‑140 (MQ=255)                                                       cTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACAATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTc                                                                             >  1:966519/1‑140 (MQ=255)                                                            tCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACAATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACAcc                                                                        >  2:851151/1‑140 (MQ=255)                                                                    tCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACAATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAAcc                                                                >  2:941224/1‑140 (MQ=255)                                                                     ccaaaaaaCAGCTCACACGCTTCGAAATACTCAACCCAAAACCCCAAAAAAAGGGGGGCCTTGCCGACAATCAACCCCGCGCGCCCCACAAAAACCCCTACCCCCCCCGCCAGTAAACCGGACTTCACACCTAACAACCa                                                               <  2:1167931/136‑1 (MQ=255)                                                                      ccTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACAATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCAc                                                              >  1:1167931/1‑140 (MQ=255)                                                                                gTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACAATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCtggtgg                                                    >  1:583095/1‑140 (MQ=255)                                                                                    tACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACAATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTgcg                                                >  1:640724/1‑140 (MQ=255)                                                                                    tACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACAATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTgcg                                                >  1:478050/1‑140 (MQ=255)                                                                                      cGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACAATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGcgcg                                              >  1:491100/1‑140 (MQ=255)                                                                                        cTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACAATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGa                                            >  1:1062794/1‑140 (MQ=255)                                                                                           cGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACAATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCt                                         >  1:1289755/1‑140 (MQ=255)                                                                                                   ttAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACAATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCAt                                 >  1:739090/1‑140 (MQ=255)                                                                                                          cAAACACCAGCGAAATGAGCGGCTTGCCGACAATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGGGCGCGGATCTAAACGCATCACctct                          >  2:359512/1‑140 (MQ=255)                                                                                                            aaCACCAGCGAAATGAGCGGCTTGCCGACAATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTgg                        >  1:283668/1‑140 (MQ=255)                                                                                                                 cAGCGAAATGAGCGGCTTGCCGACAATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTaaa                   >  1:883952/1‑140 (MQ=255)                                                                                                                   gCGAAATGAGCGGCTTGCCGACAATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTaaaaa                 >  1:836920/1‑140 (MQ=255)                                                                                                                      aaaTGAGCGGCTTGCCGACAATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACtt              >  2:727176/1‑140 (MQ=255)                                                                                                                         ggggCGGCTCCCCGACAACCAACCCCGCGCGCCCCACAAAAATCCCGATCCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTa           <  2:1289755/137‑1 (MQ=255)                                                                                                                                  tGCCGACAATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCaaa  >  1:42674/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     CGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATCAGGTCATACGCTTCGAGATACTTAACGCCAAACACCAGCGAAATGAGCGGCTTGCCGACGATCAACACCGCGAGCGCCACCAGAATACCGATTCCGCCCGCCAGTAAACCGGACTTCACACCTAACAACCACGGTCTGGTGGTGCGCGGATCTAAACGCATCACCTCTGGGTAAAAACTTTTACCCAGCAAA  >  NC_000913/3131271‑3131539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑